AB227513

Anti-COX1 / Cyclooxygenase 1 antibody

Name: Anti-COX1 / Cyclooxygenase 1 antibody (ab227513) | Abcam
Brand: Abcam Limited
SKU: AB227513
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal COX1 / Cyclooxygenase 1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTGS1.

View Alternative Names

COX1, PTGS1, Prostaglandin G/H synthase 1, Cyclooxygenase-1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1, COX-1, PGH synthase 1, PGHS-1, PHS 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody (AB227513)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody (AB227513)

Paraffin-embedded H1299 (human) xenograft tissue stained for COX1 / Cyclooxygenase 1 with ab227513 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (AB227513)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (ab227513) at 1/1000 dilution

All lanes:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (AB227513)

7.5 % SDS-PAGE gel.

All lanes:

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (ab227513) at 1/1000 dilution

All lanes:

Rat brain extract at 50 µg

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (AB227513)

7.5 % SDS-PAGE gel.

All lanes:

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (ab227513) at 1/1000 dilution

All lanes:

Mouse brain extract at 50 µg

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PTGS1. The exact immunogen used to generate this antibody is proprietary information.

P23219

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cyclooxygenase 1 (COX-1) also known as COX1 cyclooxygenase-1 COX one and the COX1 protein is an important enzyme in the conversion of arachidonic acid to prostaglandins. It is a heme-containing dimer enzyme with a molecular weight of approximately 70 kDa. COX-1 is widely expressed in most tissues and cell types where it plays a significant role in maintaining homeostatic functions. This protein is constitutively active meaning it is often active under normal physiological conditions.

Biological function summary

COX-1 is involved in producing prostaglandins that regulate a variety of normal physiological processes including gastric mucosal protection renal blood flow and platelet aggregation. Unlike COX-2 COX-1 is not induced by inflammatory stimuli and is not part of an inducible complex. It serves to maintain essential physiological functions in various organs and systems making its activity critical for cellular maintenance.

Pathways

COX-1 is primarily involved in the prostaglandin biosynthesis pathway. It converts arachidonic acid into prostaglandin H2 a precursor for other prostaglandins and thromboxanes. Thromboxane A2 produced from this pathway plays an important role in platelet aggregation and vasoconstriction linking COX-1's functions with hemostatic processes. Another protein involved in this pathway is thromboxane synthase which further processes the products of COX-1 activity.

COX-1’s role connects it closely to conditions like peptic ulcers and cardiovascular diseases. Inhibition of COX-1 by nonsteroidal anti-inflammatory drugs (NSAIDs) can lead to gastric mucosal damage contributing to the development of peptic ulcers. Additionally due to its involvement in platelet aggregation COX-1 affects thrombotic diseases. COX-1's interactions with proteins such as COX-2 become relevant in inflammation and pain management where selective inhibition of COX-2 is sought to reduce adverse effects related to COX-1.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dual cyclooxygenase and peroxidase that plays an important role in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20 : 4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed : 7947975). Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells (Probable). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18 : 2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products (By similarity).

See full target information PTGS1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40649978

2025

Exploring the Role of Peripheral Macrophages in Glioma Progression: The Metabolic Significance of Cyclooxygenase-2 (COX-2).

Applications

Unspecified application

Species

Unspecified reactive species

Jens Pietzsch,Magali Toussaint,Cornelius Kurt Donat,Alina Doctor,Sebastian Meister,Johanna Wodtke,Markus Laube,Frank Hofheinz,Jan Rix,Winnie Deuther-Conrad,Cathleen Haase-Kohn

Nature communications 15:9464 PubMed39487111

2024

Prostaglandin E-EP2/EP4 signaling induces immunosuppression in human cancer by impairing bioenergetics and ribosome biogenesis in immune cells.

Applications

Unspecified application

Species

Unspecified reactive species

Siwakorn Punyawatthananukool,Ryuma Matsuura,Thamrong Wongchang,Nao Katsurada,Tatsuaki Tsuruyama,Masaki Tajima,Yutaka Enomoto,Toshio Kitamura,Masahiro Kawashima,Masakazu Toi,Koji Yamanoi,Junzo Hamanishi,Shigeo Hisamori,Kazutaka Obama,Varodom Charoensawan,Dean Thumkeo,Shuh Narumiya

Scientific reports 13:14471 PubMed37660155

2023

Stress-mediated aggregation of disease-associated proteins in amyloid bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Sahil Chandhok,Lionel Pereira,Evgenia A Momchilova,Dane Marijan,Richard Zapf,Emma Lacroix,Avneet Kaur,Shayan Keymanesh,Charles Krieger,Timothy E Audas

Toxicology research 11:683-695 PubMed36051659

2022

Endoplasmic-reticulum-stress-induced lipotoxicity in human kidney epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tuğçe Çeker,Çağatay Yılmaz,Esma Kırımlıoglu,Mutay Aslan

Neoplasma 67:1193-1203 PubMed32305051

2020

Effect of GATA4 gene methylation on proliferation and apoptosis of SGC-7901 gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

X Ma,L Ji

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-COX1 / Cyclooxygenase 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody (AB227513)

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (AB227513)

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (AB227513)

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (AB227513)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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