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Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) is a rabbit monoclonal antibody that is used to detect COX1 / Cyclooxygenase 1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with COX1 / Cyclooxygenase 1 knockout cell line validation


Images

Immunoprecipitation - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (AB109025), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (AB109025), expandable thumbnail
  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (AB109025), expandable thumbnail
  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (AB109025), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (AB109025), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Tested
Expected
Tested
Mouse
Tested
Tested
Tested
Tested
Tested
Rat
Expected
Expected
Expected
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/10 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10 - 1/100
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/150
Notes

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/150
Notes

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/150
Notes

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Dual cyclooxygenase and peroxidase that plays an important role in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed:7947975). Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells (Probable). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products (By similarity).

Alternative names

Recommended products

Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) is a rabbit monoclonal antibody that is used to detect COX1 / Cyclooxygenase 1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with COX1 / Cyclooxygenase 1 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5866
Purification technique
Affinity purification Protein A
Dissociation constant
5.5 x 10-12 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of COX1 / Cyclooxygenase 1?


Anti-COX1 / Cyclooxygenase 1 [EPR5866] (ab109025) specifically detects a band for COX1 / Cyclooxygenase 1 (UniProt: P23219) at a molecular weight of 69kDa.

Recommended positive controls


WB: NIH/3T3, HaCaT, Neuro -2a, C2C12, A431, and L6 cell lysates.IHC-P: Human skin, human cerebrum, mouse kidney, and rat kidney tissues.ICC/IF: HeLa and Neuro-2a cells.Flow Cyt (intra): NIH/3T3 cells.IP: C2C12 cell lysate.

Trusted by the scientific community


Anti-COX1 / Cyclooxygenase 1 [EPR5866] (ab109025) was first used in a scientific publication in 2011 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed


The specificity of Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) has been confirmed by Western blot testing in PTGS1 Knockout A431 cell line, Human PTGS1 knockout A-431 cell line ab270477.



Other related products


We have a range of other formats of antibody clone [EPR5866] also available for your convenience:
ab109025, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] ab199027, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] ab199202, HRP - HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] ab199203, PE - PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] ab209581, Carrier free - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free ab219375



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cyclooxygenase 1 (COX-1) also known as COX1 cyclooxygenase-1 COX one and the COX1 protein is an important enzyme in the conversion of arachidonic acid to prostaglandins. It is a heme-containing dimer enzyme with a molecular weight of approximately 70 kDa. COX-1 is widely expressed in most tissues and cell types where it plays a significant role in maintaining homeostatic functions. This protein is constitutively active meaning it is often active under normal physiological conditions.

Biological function summary

COX-1 is involved in producing prostaglandins that regulate a variety of normal physiological processes including gastric mucosal protection renal blood flow and platelet aggregation. Unlike COX-2 COX-1 is not induced by inflammatory stimuli and is not part of an inducible complex. It serves to maintain essential physiological functions in various organs and systems making its activity critical for cellular maintenance.

Pathways

COX-1 is primarily involved in the prostaglandin biosynthesis pathway. It converts arachidonic acid into prostaglandin H2 a precursor for other prostaglandins and thromboxanes. Thromboxane A2 produced from this pathway plays an important role in platelet aggregation and vasoconstriction linking COX-1's functions with hemostatic processes. Another protein involved in this pathway is thromboxane synthase which further processes the products of COX-1 activity.

Associated diseases and disorders

COX-1’s role connects it closely to conditions like peptic ulcers and cardiovascular diseases. Inhibition of COX-1 by nonsteroidal anti-inflammatory drugs (NSAIDs) can lead to gastric mucosal damage contributing to the development of peptic ulcers. Additionally due to its involvement in platelet aggregation COX-1 affects thrombotic diseases. COX-1's interactions with proteins such as COX-2 become relevant in inflammation and pain management where selective inhibition of COX-2 is sought to reduce adverse effects related to COX-1.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Immunoprecipitation - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Immunoprecipitation - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    ab109025 (purified) at 1:20 dilution (0.8μg) immunoprecipitating COX1 / Cyclooxygenase 1 in C2C12 whole cell lysate.

    Lane 1 (input): C2C12 (Mouse myoblasts myoblast) whole cell lysate,10μg
    Lane 2 (+): ab109025 & C2C12 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109025 in C2C12 whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Predicted band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution.

    PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/10000 dilution

    Lane 1: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: L6 (Rat skeletal muscle myoblast) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa

  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/2000 dilution

    Lane 1: HaCaT (Human skin keratinocyte) whole cell lysates at 20 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa

  • Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling COX1 / Cyclooxygenase 1 with purified ab109025 at 1/100 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution.

    PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution.

    PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling COX1 with purified ab109025 at 1/50. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only.

  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    All lanes: Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/1000 dilution

    Lane 1: NIH/3T3 cell lysate at 10 µg

    Lane 2: HaCaT cell lysate at 10 µg

    Lane 3: Neuro 2a cell lysate at 10 µg

    Lane 4: C2C12 cell lysate at 10 µg

    Lane 5: A431 cell lysate at 10 µg

    Lane 6: L6 cell lysate at 10 µg

    Predicted band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Unpurified ab109025 at 1/250 dilution staining COX1 / Cyclooxygenase 1 in human skin by immunohistochemistry, paraffin-embedded tissue.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Unpurified ab109025 at 1/100 dilution staining COX1 / Cyclooxygenase 1 in HeLa cells by Immunofluorescence.

  • Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Overlay histogram showing NIH/3T3 cells stained with unpurified ab109025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109025, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • OI-RD Scanning - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    OI-RD Scanning - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025), expandable thumbnail

    Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    False colour image of Western blot: Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.

    In Western blot, ab109025 was shown to bind specifically to COX1 / Cyclooxygenase 1. A band was observed at 70 kDa in wild-type A431 cell lysates with no signal observed at this size in PTGS1 knockout cell line Human PTGS1 knockout A-431 cell line ab270477 (knockout cell lysate ab270500).

    To generate this image, wild-type and PTGS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    Lanes 1 - 4: Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free (Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free ab219375) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/1000 dilution

    Lane 1: Wild-type A431 cell lysate at 20 µg

    Lane 2: Western blot - Human PTGS1 knockout A-431 cell lysate (ab270500)

    Lane 2: PTGS1 knockout A431 cell lysate at 20 µg

    Lane 2: Western blot - Human PTGS1 knockout A-431 cell line (Human PTGS1 knockout A-431 cell line ab270477)

    Lane 3: C2C12 cell lysate at 20 µg

    Lane 4: MOLT-4 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 70 kDa

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Product protocols

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