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AB228734

Anti-COX10 antibody

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(2 Publications )

Rabbit Polyclonal COX10 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Protoheme IX farnesyltransferase, mitochondrial.
1 Images
Western blot - Anti-COX10 antibody (AB228734)
  • WB

Supplier Data

Western blot - Anti-COX10 antibody (AB228734)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-COX10 antibody (ab228734) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg

Lane 3:

IMR32 whole cell extract at 30 µg

Lane 4:

SK-N-AS whole cell extract at 30 µg

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Protoheme IX farnesyltransferase, mitochondrial. The exact immunogen used to generate this antibody is proprietary information.

Q12887

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COX10 also known as Protoheme IX farnesyltransferase plays a significant role in the heme A biosynthesis pathway which is essential for the maturation of cytochrome c oxidase (Complex IV). The molecular mass of COX10 is approximately 48 kDa. It is an inner mitochondrial membrane protein primarily expressed in tissues with high metabolic rates such as muscles and the liver. COX10 facilitates the conversion of protoheme IX into heme O by transferring a farnesyl group a critical step in heme A production.
Biological function summary

COX10 functions as part of the cytochrome c oxidase complex serving an important role in cellular respiration. This enzyme complex regulates the final step of the mitochondrial electron transport chain which is vital for ATP production. Heme A synthesized with the help of COX10 is necessary for the proper function of cytochrome c oxidase which catalyzes the reduction of oxygen to water. Therefore COX10 indirectly contributes to the maintenance of cellular energy homeostasis.

Pathways

COX10 is integral to the oxidative phosphorylation pathway a central route in cellular metabolism. Through its involvement in this pathway COX10 affects the electron transport chain and directly impacts ATP synthesis. COX10 interacts with other cytochrome c oxidase subunits such as COX15 which also participates in heme A biosynthesis. These interactions emphasize the role of COX10 in coordination with other proteins to support efficient energy production.

Dysfunction in COX10 can contribute to disorders like mitochondrial complex IV deficiency and Leigh syndrome a severe neurological disorder. Mutations in the COX10 gene can disrupt cytochrome c oxidase assembly or activity leading to reduced ATP production and ensuing energy deficits in cells. This dysfunctional energy metabolism impacts tissues with high energy demands linking COX10 to disease pathology. Additionally COX15 and its role in heme A biosynthesis may further elucidate the disease mechanisms associated with COX10-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts protoheme IX and farnesyl diphosphate to heme O.
See full target information Protoheme IX farnesyltransferase, mitochondrial

Alternative Names

Heme O synthase, COX10
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Scientific reports 13:8034 PubMed37198225

2023

Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina M Pusec,Vladimir Ilievski,Adam De Jesus,Zeenat Farooq,Joseph L Zapater,Nadia Sweis,Hagar Ismail,Md Wasim Khan,Hossein Ardehali,Jose Cordoba-Chacon,Brian T Layden

Journal of biological engineering 16:2 PubMed34986861

2022

Knockdown of NCK1-AS1 inhibits the development of atherosclerosis by targeting miR-1197/COX10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhang,Juncheng Wang,Lei Du,Lufei Shao,Yourui Zou,Haibo Liu,Jinfang Liu
View all publications
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Product promise

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