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AB201082

Anti-COX15 antibody

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(1 Publication)

Rabbit Polyclonal COX15 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human COX15 aa 200-250.

View Alternative Names

Heme A synthase COX15, HAS, Cytochrome c oxidase assembly protein COX15 homolog, COX15

1 Images
Western blot - Anti-COX15 antibody (AB201082)
  • WB

Supplier Data

Western blot - Anti-COX15 antibody (AB201082)

All lanes:

Western blot - Anti-COX15 antibody (ab201082)

Lane 1:

HEK293T whole cell lysate

Lane 2:

Mouse Raw 264.7 whole cell lysate

Lane 3:

Rat PC12 whole cell lysate

Predicted band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human COX15 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q7KZN9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab201082 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The COX15 protein also known as heme A:farnesyltransferase (protein of mass approximately 46 kDa) plays an important role in the mitochondrial respiratory chain. COX15 contributes to the biosynthesis of heme A an essential cofactor for cytochrome c oxidase also called complex IV which is the last enzyme in the electron transport chain of mitochondria. COX15 is highly expressed in tissues with high energy demands like heart and muscles due to their dependence on mitochondrial oxidative phosphorylation.
Biological function summary

COX15 is integral for the assembly and function of cytochrome c oxidase as it facilitates the conversion of heme O to heme A making it a critical component in the terminal step of electron transport in mitochondria. It forms part of a complex enzymatic machinery that ensures the generation of cellular ATP through oxidative phosphorylation. The presence of COX15 is necessary for the efficient assembly of cytochrome c oxidase which is important for cellular energy production.

Pathways

COX15 significantly impacts the electron transport chain and plays a pivotal role in the oxidative phosphorylation pathway. It maintains a close association with other subunits of cytochrome c oxidase enabling efficient electron transfer and proton pumping across the inner mitochondrial membrane. Proteins like COX10 and SURF1 collaborate within these pathways to ensure proper formation and activity of the cytochrome c oxidase complex influencing both energetic metabolism and apoptosis regulation.

COX15 mutations have been linked to mitochondrial disorders such as Leigh syndrome and hypertrophic cardiomyopathy. These conditions result from compromised electron transport chain functionality leading to impaired energy generation and increased oxidative stress. The connection between COX15 and SURF1 another protein involved in cytochrome c oxidase assembly is significant as defects in these proteins often result in overlapping clinical features in mitochondrial diseases. Researchers actively study COX15 to understand its role in these pathologies better and investigate potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the second reaction in the biosynthesis of heme A, a prosthetic group of mitochondrial cytochrome c oxidase (CcO) (PubMed : 12474143). Heme A is synthesized from heme B by two sequential enzymatic reactions catalyzed by heme O synthase (HOS) and heme A synthase (HAS). HAS catalyzes the conversion of heme O to heme A by two successive hydroxylations of the methyl group at C8, in a reaction that involves matrix ferredoxin and ferredoxin reductase. The first hydroxylation forms heme I, the second hydroxylation results in an unstable dihydroxymethyl group, which spontaneously dehydrates, resulting in the formyl group of heme A (By similarity).
See full target information COX15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology 11: PubMed35336822

2022

AMPK Activation Is Indispensable for the Protective Effects of Caloric Restriction on Left Ventricular Function in Postinfarct Myocardium.

Applications

Unspecified application

Species

Unspecified reactive species

Bernd Niemann,Ruping Pan,Hassan Issa,Andreas Simm,Rainer Schulz,Susanne Rohrbach
View all publications
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