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AB190251

Anti-COX16 antibody

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(1 Publication)

Rabbit Polyclonal COX16 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COX16 aa 50 to C-terminus.

View Alternative Names

C14orf112, HSPC203, PTD019, COX16, hCOX16

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX16 antibody (AB190251)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX16 antibody (AB190251)

Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue labeling COX16 in the cytoplasm and membrane of smooth muscle cells with ab190251 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-COX16 antibody (AB190251)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX16 antibody (AB190251)

Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling COX16 in the mitochondria with ab190251 at 2 μg/ml.

Western blot - Anti-COX16 antibody (AB190251)
  • WB

Supplier Data

Western blot - Anti-COX16 antibody (AB190251)

All lanes:

Western blot - Anti-COX16 antibody (ab190251) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate

Predicted band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human COX16 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P0S2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COX16 also known as cytochrome c oxidase assembly factor COX16 is a mitochondrial membrane protein with a molecular mass of approximately 16 kDa. This protein is expressed primarily in tissues with high energy demands such as skeletal muscles heart and brain. It resides in the inner mitochondrial membrane where it assists in the stabilization and assembly of cytochrome c oxidase an important component of the mitochondrial respiratory chain.
Biological function summary

COX16 plays a role in facilitating the assembly of cytochrome c oxidase which is a multi-subunit complex. This enzyme complex is responsible for the final step in the electron transport chain a process critical for ATP production in cells. COX16 contributes to the incorporation of subunit Cox1 into the growing enzyme complex ensuring proper enzyme functionality and cellular energy production.

Pathways

COX16 is integral to mitochondrial oxidative phosphorylation and respiratory chain complexes formation. It interacts closely with other proteins such as SURF1 and LRPPRC which are also involved in cytochrome c oxidase assembly. Dysfunction in these pathways can lead to impaired energy production and metabolic disturbances at the cellular level.

COX16 mutations have been linked to disorders like mitochondrial complex IV deficiency and Leigh syndrome. These diseases manifest with neurological and muscular symptoms due to impaired energy metabolism. COX16's dysfunction in these conditions often involves interactions with proteins like COX10 and COX2 further implicating the cytochrome oxidase complex's role in disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase (PubMed : 29355485, PubMed : 29381136, PubMed : 33169484). Promotes the insertion of copper into the active site of cytochrome c oxidase subunit II (MT-CO2/COX2) (PubMed : 29355485, PubMed : 29381136). Interacts specifically with newly synthesized MT-CO2/COX and its copper center-forming metallochaperones SCO1, SCO2 and COA6 (PubMed : 29381136). Probably facilitates MT-CO2/COX2 association with the MITRAC assembly intermediate containing MT-CO1/COX1, thereby participating in merging the MT-CO1/COX1 and MT-CO2/COX2 assembly lines (PubMed : 29381136).
See full target information COX16
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 161:27 PubMed39192374

2024

The novel circFKBP8/miR-432-5p/E2F7 cascade functions as a regulatory network in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongkui Jin,Wang Xu,Kunlin Yu,Cailu Luo,Xiaodan Luo,Tao Lian,Changshan Liu
View all publications
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