Rabbit Polyclonal COX20 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COX20 aa 50 to C-terminus.
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- Frontiers in neurology 13:8739432022Compound Heterozygous Variants Impair the Function of Mitochondrial Complex IV to Cause a Syndrome Involving Ophthalmoplegia and Visual Failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeizheng Li,Dandan Guo,Xiufang Zhang,Kunqian Ji,Hongbo Lv,Yanli Zhang,Zhichao Chen,Jun Ma,Yaofeng Fang,Yiming LiuPubMed 35651336
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human COX20 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q5RI15
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase (PubMed:23125284). Acts as a chaperone in the early steps of cytochrome c oxidase subunit II (MT-CO2/COX2) maturation, stabilizing the newly synthesized protein and presenting it to metallochaperones SCO1/2 which in turn facilitates the incorporation of the mature MT-CO2/COX2 into the assembling CIV holoenzyme (PubMed:24403053).
Alternative names
COX20, FAM36A
Recommended products
Rabbit Polyclonal COX20 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COX20 aa 50 to C-terminus.
Alternative names
COX20, FAM36A
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human COX20 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q5RI15
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
COX20 also known as FAM36A is a mitochondrial protein with a mass of around 11 kDa. COX20 helps the assembly and stability of cytochrome c oxidase (COX) the terminal enzyme of the mitochondrial respiratory chain. Researchers found COX20 widely expressed in tissues with high metabolic rates including the heart skeletal muscle and brain. It functions by acting as a chaperone specifically aiding the insertion of COX2 into the mitochondrial inner membrane.
- Biological function summary
COX20 facilitates complex IV's assembly by ensuring proper incorporation of subunits into the holoenzyme. This process is important for efficient mitochondrial oxidative phosphorylation which produces ATP. COX20 is part of a mitochondrial protein complex that incorporates the COX2 subunit into the cytochrome c oxidase complex. Disturbances in COX20's function can lead to defects in energy production highlighting its role in cellular metabolism.
- Pathways
Scientists have positioned COX20 in the electron transport chain where it assists cytochrome c oxidase integration an important step in cellular respiration. COX20 interacts with other proteins like COX15 and SURF1 which are also essential components in the electron transport pathway coordinating the assembly of the cytochrome oxidase complex. Efficient pathway function ensures energy metabolism in cells affecting nutrient utilization and energy balance.
- Associated diseases and disorders
Abnormalities in COX20 have associations with mitochondrial disorders particularly COX deficiency which can lead to various neuromuscular diseases. Research shows that defective COX20 impacts neurological and muscular functions revealing links to conditions such as Leigh syndrome and encephalomyopathy. Alongside proteins like SCO1 impaired COX20 can alter copper transportation and incorporation within cytochrome c oxidase pointing to its role in disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-COX20 antibody - C-terminal (ab224570)
All lanes: Western blot - Anti-COX20 antibody - C-terminal (ab224570) at 0.4 µg/mL
Lane 1: A-431 cell line
Lane 2: HEK 293 cell line
Predicted band size: 13 kDa
Immunocytochemistry/ Immunofluorescence - Anti-COX20 antibody - C-terminal (ab224570)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for COX20 (green) using ab224570 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX20 antibody - C-terminal (ab224570)
Paraffin-embedded human kidney tissue stained for COX20 with ab224570 at 1/50 dilution in immunohistochemical analysis.
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