AB224570

Anti-COX20 antibody - C-terminal

Name: Anti-COX20 antibody - C-terminal (ab224570) | Abcam
Brand: Abcam Limited
SKU: AB224570
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal COX20 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COX20 aa 50 to C-terminus.

View Alternative Names

FAM36A, COX20

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-COX20 antibody - C-terminal (AB224570)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX20 antibody - C-terminal (AB224570)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for COX20 (green) using ab224570 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX20 antibody - C-terminal (AB224570)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX20 antibody - C-terminal (AB224570)

Paraffin-embedded human kidney tissue stained for COX20 with ab224570 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-COX20 antibody - C-terminal (AB224570)

All lanes:

Western blot - Anti-COX20 antibody - C-terminal (ab224570) at 0.4 µg/mL

Lane 1:

A-431 cell line

Lane 2:

HEK 293 cell line

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human COX20 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5RI15

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COX20 also known as FAM36A is a mitochondrial protein with a mass of around 11 kDa. COX20 helps the assembly and stability of cytochrome c oxidase (COX) the terminal enzyme of the mitochondrial respiratory chain. Researchers found COX20 widely expressed in tissues with high metabolic rates including the heart skeletal muscle and brain. It functions by acting as a chaperone specifically aiding the insertion of COX2 into the mitochondrial inner membrane.

Biological function summary

COX20 facilitates complex IV's assembly by ensuring proper incorporation of subunits into the holoenzyme. This process is important for efficient mitochondrial oxidative phosphorylation which produces ATP. COX20 is part of a mitochondrial protein complex that incorporates the COX2 subunit into the cytochrome c oxidase complex. Disturbances in COX20's function can lead to defects in energy production highlighting its role in cellular metabolism.

Pathways

Scientists have positioned COX20 in the electron transport chain where it assists cytochrome c oxidase integration an important step in cellular respiration. COX20 interacts with other proteins like COX15 and SURF1 which are also essential components in the electron transport pathway coordinating the assembly of the cytochrome oxidase complex. Efficient pathway function ensures energy metabolism in cells affecting nutrient utilization and energy balance.

Abnormalities in COX20 have associations with mitochondrial disorders particularly COX deficiency which can lead to various neuromuscular diseases. Research shows that defective COX20 impacts neurological and muscular functions revealing links to conditions such as Leigh syndrome and encephalomyopathy. Alongside proteins like SCO1 impaired COX20 can alter copper transportation and incorporation within cytochrome c oxidase pointing to its role in disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase (PubMed : 23125284). Acts as a chaperone in the early steps of cytochrome c oxidase subunit II (MT-CO2/COX2) maturation, stabilizing the newly synthesized protein and presenting it to metallochaperones SCO1/2 which in turn facilitates the incorporation of the mature MT-CO2/COX2 into the assembling CIV holoenzyme (PubMed : 24403053).

See full target information COX20

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neurology 13:873943 PubMed35651336

2022

Compound Heterozygous Variants Impair the Function of Mitochondrial Complex IV to Cause a Syndrome Involving Ophthalmoplegia and Visual Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Peizheng Li,Dandan Guo,Xiufang Zhang,Kunqian Ji,Hongbo Lv,Yanli Zhang,Zhichao Chen,Jun Ma,Yaofeng Fang,Yiming Liu

Journal of virology 92: PubMed30068640

2018

Epstein-Barr Virus-Induced Nodules on Viral Replication Compartments Contain RNA Processing Proteins and a Viral Long Noncoding RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Richard Park,George Miller

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COX20 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-COX20 antibody - C-terminal (AB224570)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX20 antibody - C-terminal (AB224570)

Western blot - Anti-COX20 antibody - C-terminal (AB224570)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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