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AB317183

Anti-COX4 antibody [HL2264]

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Rabbit Recombinant Monoclonal COX IV antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human, Rat, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human COX4I1.

View Alternative Names

COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-COX4 antibody [HL2264] (AB317183)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX4 antibody [HL2264] (AB317183)

ab317183 detects COX4 protein at mitochondria by immunofluorescent analysis. Sample : HeLa cells were fixed in ice-cold MeOH for 5 min. Green : COX4 stained by ab317183 diluted at 1 : 500. Red : alpha Tubulin a cytoskeleton marker stained by alpha Tubulin antibody [GT114] diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX4 antibody [HL2264] (AB317183)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX4 antibody [HL2264] (AB317183)

ab317183 detects COX4 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. COX4 stained by ab317183 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-COX4 antibody [HL2264] (AB317183)
  • WB

Supplier Data

Western blot - Anti-COX4 antibody [HL2264] (AB317183)

Whole cell extract (30 ug) was separated by 15% SDS-PAGE and the membrane was blotted with ab317183 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-COX4 antibody [HL2264] (ab317183) at 1/1000 dilution

All lanes:

Raw264.7 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-COX4 antibody [HL2264] (AB317183)
  • WB

Supplier Data

Western blot - Anti-COX4 antibody [HL2264] (AB317183)

Rat tissue extract (50 ug) was separated by 15% SDS-PAGE and the membrane was blotted with ab317183 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-COX4 antibody [HL2264] (ab317183) at 1/1000 dilution

All lanes:

Rat stomach tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-COX4 antibody [HL2264] (AB317183)
  • WB

Supplier Data

Western blot - Anti-COX4 antibody [HL2264] (AB317183)

Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE and the membrane was blotted with ab317183 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-COX4 antibody [HL2264] (ab317183) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2264

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat, Zebrafish

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human COX4I1.

P13073

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.
Biological function summary

COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.

Pathways

COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.

COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information COX4I1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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