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Mouse Monoclonal COX IV antibody. Suitable for WB, Flow Cyt, ICC/IF, IHC-Fr and reacts with Cow, Human samples. Cited in 51 publications.


Images

Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (AB110261), expandable thumbnail
  • Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (AB110261), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (AB110261), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (AB110261), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (AB110261), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow CytICC/IFIHC-Fr
Human
Tested
Tested
Tested
Tested
Cow
Tested
Expected
Expected
Expected
Zebrafish
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Cow
Dilution info
1 µg/mL
Notes

-

Species
Human
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Zebrafish
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Zebrafish
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

(heat-induced antigen-retrieval improves signal)

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Zebrafish
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/800
Notes

-

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Zebrafish
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Additional Targets

COX4I2

Alternative names

Recommended products

Mouse Monoclonal COX IV antibody. Suitable for WB, Flow Cyt, ICC/IF, IHC-Fr and reacts with Cow, Human samples. Cited in 51 publications.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
10G8D12C12
Purity
IgG fraction
Light chain type
kappa
Concentration
Loading...
Purification notes

ab110261 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110261 was judged as near homogeneity by SDS PAGE.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The COX4 protein also known as Cytochrome c oxidase subunit 4 along with its isoform COX4L2 forms part of the electron transport chain in mitochondria. COX4 has a molecular mass of approximately 20.8 kDa and is found in various tissues especially in high-energy-demand organs like the heart and liver. Both COX4 and COX4L2 belong to the inner mitochondrial membrane and play a role in the enzyme complex Cytochrome c oxidase essential for efficient cellular respiration.

Biological function summary

COX4 and COX4L2 contribute to the terminal stage of the mitochondrial electron transport chain. They form core components of the Cytochrome c oxidase complex also called complex IV which facilitates the transfer of electrons from reduced cytochrome c to oxygen. This process not only aids in the production of water but also helps create the proton gradient used by ATP synthase to generate ATP the primary energy currency in cells.

Pathways

Both COX4 and COX4L2 integrate into key metabolic pathways such as oxidative phosphorylation and the respiratory chain. Their role in the electron transport chain connects them to other mitochondrial proteins like cytochrome c and ATP synthase. This collaboration is important for the efficient conversion of nutrients into usable cellular energy supporting essential metabolic functions.

Associated diseases and disorders

Disruptions in the function of COX4 and COX4L2 relate to conditions such as mitochondrial myopathy and Leigh syndrome. Mutations or deficiencies in these proteins can impair electron transport and reduce ATP generation leading to muscle weakness or neurodegenerative symptoms. Cytochrome c and other components of the electron transport chain often exhibit related dysfunctions in these mitochondrial diseases.

Product promise

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5 product images

  • Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261), expandable thumbnail

    Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)

    ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).

  • Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (ab110261), expandable thumbnail

    Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (ab110261)

    COX4 + COX4L2 Western blot staining using mouse Anti-COX4 + COX4L2 antibody

    All lanes: Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (ab110261) at 1 µg/mL

    Lane 1: Isolated mitochondria from Human heart at 5 µg

    Lane 2: Isolated mitochondria from Bovine heart at 1 µg

    Lane 3: Isolated mitochondria from HepG2 at 20 µg

    Predicted band size: 20 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)

    ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.

  • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)

    ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.

  • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)

    ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.

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Product protocols

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