Mouse Monoclonal COX IV antibody. Suitable for WB, Flow Cyt, ICC/IF, IHC-Fr and reacts with Cow, Human samples. Cited in 51 publications.
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
WB | Flow Cyt | ICC/IF | IHC-Fr | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Cow | Tested | Expected | Expected | Expected |
Zebrafish | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes (heat-induced antigen-retrieval improves signal) |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/800 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Select an associated product type
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
COX4I2
COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1
Mouse Monoclonal COX IV antibody. Suitable for WB, Flow Cyt, ICC/IF, IHC-Fr and reacts with Cow, Human samples. Cited in 51 publications.
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
ab110261 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110261 was judged as near homogeneity by SDS PAGE.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
The COX4 protein also known as Cytochrome c oxidase subunit 4 along with its isoform COX4L2 forms part of the electron transport chain in mitochondria. COX4 has a molecular mass of approximately 20.8 kDa and is found in various tissues especially in high-energy-demand organs like the heart and liver. Both COX4 and COX4L2 belong to the inner mitochondrial membrane and play a role in the enzyme complex Cytochrome c oxidase essential for efficient cellular respiration.
COX4 and COX4L2 contribute to the terminal stage of the mitochondrial electron transport chain. They form core components of the Cytochrome c oxidase complex also called complex IV which facilitates the transfer of electrons from reduced cytochrome c to oxygen. This process not only aids in the production of water but also helps create the proton gradient used by ATP synthase to generate ATP the primary energy currency in cells.
Both COX4 and COX4L2 integrate into key metabolic pathways such as oxidative phosphorylation and the respiratory chain. Their role in the electron transport chain connects them to other mitochondrial proteins like cytochrome c and ATP synthase. This collaboration is important for the efficient conversion of nutrients into usable cellular energy supporting essential metabolic functions.
Disruptions in the function of COX4 and COX4L2 relate to conditions such as mitochondrial myopathy and Leigh syndrome. Mutations or deficiencies in these proteins can impair electron transport and reduce ATP generation leading to muscle weakness or neurodegenerative symptoms. Cytochrome c and other components of the electron transport chain often exhibit related dysfunctions in these mitochondrial diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).
COX4 + COX4L2 Western blot staining using mouse Anti-COX4 + COX4L2 antibody
All lanes: Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (ab110261) at 1 µg/mL
Lane 1: Isolated mitochondria from Human heart at 5 µg
Lane 2: Isolated mitochondria from Bovine heart at 1 µg
Lane 3: Isolated mitochondria from HepG2 at 20 µg
Predicted band size: 20 kDa
ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.
ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com