Mouse Monoclonal COX IV antibody. Suitable for WB, Flow Cyt, ICC/IF, IHC-Fr and reacts with Cow, Human samples. Cited in 51 publications.
Images
![Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (AB110261), expandable thumbnail](https://content.abcam.com/products/images/cox4-cox4l2-antibody-10g8d12c12-mitochondrial-marker-ab110261--flow-cytometry-img122598.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (AB110261)")
Publications
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- Frontiers in cell and developmental biology 10:7862682022Applying Sodium Carbonate Extraction Mass Spectrometry to Investigate Defects in the Mitochondrial Respiratory Chain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid R L Robinson,Daniella H Hock,Linden Muellner-Wong,Roopasingam Kugapreethan,Boris Reljic,Elliot E Surgenor,Carlos H M Rodrigues,Nikeisha J Caruana,David A StroudPubMed 35300415
- Biomedicines 10:2022Genetic Complementation of ATP Synthase Deficiency Due to Dysfunction of TMEM70 Assembly Factor in Rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Marković,Kateřina Tauchmannová,Miroslava Šimáková,Petr Mlejnek,Vilma Kaplanová,Petr Pecina,Alena Pecinová,František Papoušek,František Liška,Jan Šilhavý,Jana Mikešová,Jan Neckář,Josef Houštěk,Michal Pravenec,Tomáš MráčekPubMed 35203486
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Optic atrophy-associated TMEM126A is an assembly factor for the ND4-module of mitochondrial complex I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuke E Formosa,Boris Reljic,Alice J Sharpe,Daniella H Hock,Linden Muellner-Wong,David A Stroud,Michael T RyanPubMed 33879611
- Med (New York, N.Y.) 2:49-732021Fatal perinatal mitochondrial cardiac failure caused by recurrent duplications in the locus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnn E Frazier,Alison G Compton,Yoshihito Kishita,Daniella H Hock,AnneMarie E Welch,Sumudu S C Amarasekera,Rocio Rius,Luke E Formosa,Atsuko Imai-Okazaki,David Francis,Min Wang,Nicole J Lake,Simone Tregoning,Jafar S Jabbari,Alexis Lucattini,Kazuhiro R Nitta,Akira Ohtake,Kei Murayama,David J Amor,George McGillivray,Flora Y Wong,Marjo S van der Knaap,R Jeroen Vermeulen,Esko J Wiltshire,Janice M Fletcher,Barry Lewis,Gareth Baynam,Carolyn Ellaway,Shanti Balasubramaniam,Kaustuv Bhattacharya,Mary-Louise Freckmann,Susan Arbuckle,Michael Rodriguez,Ryan J Taft,Simon Sadedin,Mark J Cowley,André E Minoche,Sarah E Calvo,Vamsi K Mootha,Michael T Ryan,Yasushi Okazaki,David A Stroud,Cas Simons,John Christodoulou,David R ThorburnPubMed 33575671
- Acta neuropathologica 141:511-5262021Lipids, lysosomes and mitochondria: insights into Lewy body formation from rare monogenic disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Erskine,David Koss,Viktor I Korolchuk,Tiago F Outeiro,Johannes Attems,Ian McKeithPubMed 33515275
- Scientific reports 10:153362020Decoding mitochondrial heterogeneity in single muscle fibres by imaging mass cytometry.
- Acta neuropathologica communications 8:1032020Complex I reductions in the nucleus basalis of Meynert in Lewy body dementia: the role of Lewy bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher Hatton et. al.PubMed 32646480
- Neurology. Genetics 6:e4642020COX deficiency and leukoencephalopathy due to a novel homozygous mutation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarola Hedberg-Oldfors et. al.PubMed 32637636
- Cell reports 31:1075412020Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuke E Formosa,Linden Muellner-Wong,Boris Reljic,Alice J Sharpe,Thomas D Jackson,Traude H Beilharz,Diana Stojanovski,Michael Lazarou,David A Stroud,Michael T RyanPubMed 32320651
- Molecular & cellular proteomics : MCP 19:1145-11602020HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniella H Hock et. al.PubMed 32317297
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | Flow Cyt | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Cow | Tested | Expected | Expected | Expected |
| Zebrafish | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes (heat-induced antigen-retrieval improves signal) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/800 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Additional Targets
COX4I2
Alternative names
COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1
Recommended products
Mouse Monoclonal COX IV antibody. Suitable for WB, Flow Cyt, ICC/IF, IHC-Fr and reacts with Cow, Human samples. Cited in 51 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 10G8D12C12
- Purity
- IgG fraction
- Light chain type
- kappa
- Concentration
- Purification notes
ab110261 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110261 was judged as near homogeneity by SDS PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The COX4 protein also known as Cytochrome c oxidase subunit 4 along with its isoform COX4L2 forms part of the electron transport chain in mitochondria. COX4 has a molecular mass of approximately 20.8 kDa and is found in various tissues especially in high-energy-demand organs like the heart and liver. Both COX4 and COX4L2 belong to the inner mitochondrial membrane and play a role in the enzyme complex Cytochrome c oxidase essential for efficient cellular respiration.
- Biological function summary
COX4 and COX4L2 contribute to the terminal stage of the mitochondrial electron transport chain. They form core components of the Cytochrome c oxidase complex also called complex IV which facilitates the transfer of electrons from reduced cytochrome c to oxygen. This process not only aids in the production of water but also helps create the proton gradient used by ATP synthase to generate ATP the primary energy currency in cells.
- Pathways
Both COX4 and COX4L2 integrate into key metabolic pathways such as oxidative phosphorylation and the respiratory chain. Their role in the electron transport chain connects them to other mitochondrial proteins like cytochrome c and ATP synthase. This collaboration is important for the efficient conversion of nutrients into usable cellular energy supporting essential metabolic functions.
- Associated diseases and disorders
Disruptions in the function of COX4 and COX4L2 relate to conditions such as mitochondrial myopathy and Leigh syndrome. Mutations or deficiencies in these proteins can impair electron transport and reduce ATP generation leading to muscle weakness or neurodegenerative symptoms. Cytochrome c and other components of the electron transport chain often exhibit related dysfunctions in these mitochondrial diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
![Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261), expandable thumbnail](https://content.abcam.com/products/images/cox4-cox4l2-antibody-10g8d12c12-mitochondrial-marker-ab110261--flow-cytometry-img122598.jpg "Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)")
Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).
![Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (ab110261), expandable thumbnail](https://content.abcam.com/products/images/cox4-cox4l2-antibody-10g8d12c12-mitochondrial-marker-ab110261--western-blot-img70759.gif "Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (ab110261)")
Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (ab110261)
COX4 + COX4L2 Western blot staining using mouse Anti-COX4 + COX4L2 antibody
All lanes: Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (ab110261) at 1 µg/mL
Lane 1: Isolated mitochondria from Human heart at 5 µg
Lane 2: Isolated mitochondria from Bovine heart at 1 µg
Lane 3: Isolated mitochondria from HepG2 at 20 µg
Predicted band size: 20 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261), expandable thumbnail](https://content.abcam.com/products/images/cox4-cox4l2-antibody-10g8d12c12-mitochondrial-marker-ab110261--immunocytochemistry-immunofluorescence-img94622.gif "Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)")
Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.
![Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261), expandable thumbnail](https://content.abcam.com/products/images/cox4-cox4l2-antibody-10g8d12c12-mitochondrial-marker-ab110261--immunohistochemistry-frozen-sections-img123472.gif "Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)")
Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
![Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261), expandable thumbnail](https://content.abcam.com/products/images/cox4-cox4l2-antibody-10g8d12c12-mitochondrial-marker-ab110261--immunohistochemistry-frozen-sections-img49421.gif "Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)")
Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
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