Skip to main content

Mouse Monoclonal COX4, mitochondrial antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 21 publications.


Images

Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (AB110272), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Saccharomyces cerevisiae
Tested

Tested
Tested

Species
Saccharomyces cerevisiae
Dilution info
1 µg/mL
Notes

-

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of COX2 and heme A of COX1 to the active site in COX1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

YGL187C, G1362, COX4, Cytochrome c oxidase polypeptide IV

Recommended products

Mouse Monoclonal COX4, mitochondrial antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 21 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
1A12A12
Purity
IgG fraction
Light chain type
kappa
Concentration
Loading...
Purification notes

Near homogeneity as judged by SDS-PAGE. ab110272 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

COX4 also known as cytochrome c oxidase subunit IV is a component of the mitochondrial respiratory chain. It has an approximate molecular weight of 17 kDa. COX4 is expressed in high levels in tissues with abundant energy requirements such as heart muscle and brain. This protein is part of the cytochrome c oxidase (complex IV) the last enzyme in the mitochondrial electron transport chain which plays an important role in cellular respiration.

Biological function summary

The cytochrome c oxidase complex facilitates the transfer of electrons from cytochrome c to oxygen ultimately generating water. This process contributes to the proton gradient across the mitochondrial membrane necessary for ATP synthesis. COX4 serves as a regulatory subunit that can influence the efficiency of the electron transfer process. This complex is vital for maintaining the balance of energy production in cells and has a role in adaptive responses to varying energy demands.

Pathways

COX4 functions in the oxidative phosphorylation pathway which is critical for ATP production in eukaryotic cells. This pathway includes several protein complexes with COX4 being a part of the terminal complex IV. It interacts with other proteins such as cytochrome c and ATP synthase linking electron transport to ATP production. In turn this pathway supports various biological functions and maintains cellular energy homeostasis.

Associated diseases and disorders

Dysfunctional COX4 is associated with mitochondrial diseases that often lead to compromised energy production. Conditions such as mitochondrial myopathy and cytochrome c oxidase deficiency have been linked to functional anomalies in COX4. These disorders can affect muscles and neural tissues leading to symptoms such as muscle weakness and neurological impairments. COX4 acts with proteins like cytochrome c and other subunits of the cytochrome c oxidase complex in these disease mechanisms influencing the severity and progression of these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (ab110272), expandable thumbnail

    Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (ab110272)

    All lanes: Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (ab110272) at 1 µg/mL

    All lanes: Mitochondria from yeast membrane extract at 20 µg

    Predicted band size: 17 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com