Mouse Monoclonal COX5A antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, Cow samples. Cited in 54 publications.
Images
![Western blot - Anti-COX5A antibody [6E9B12D5] (AB110262), expandable thumbnail](https://content.abcam.com/products/images/cox5a-antibody-6e9b12d5-ab110262--western-blot-img122822.gif "Western blot - Anti-COX5A antibody [6E9B12D5] (AB110262)")
![Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (AB110262), expandable thumbnail](https://content.abcam.com/products/images/cox5a-antibody-6e9b12d5-ab110262--flow-cytometry-img75075.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (AB110262)")
Publications
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- PloS one 18:e02914422023Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPablo Serrano-Lorenzo,Dino Gobelli,Rocío Garrido-Moraga,María J Esteban-Amo,José R López-López,Antonio Orduña,Miguel A de la Fuente,Miguel A Martín,María SimarroPubMed 37725617
- Archives of toxicology 97:1927-19412023Metabolic impact of genetic and chemical ADP/ATP carrier inhibition in renal proximal tubule epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte A Hoogstraten,Maaike M E Jacobs,Guido de Boer,Melissa A E van de Wal,Werner J H Koopman,Jan A M Smeitink,Frans G M Russel,Tom J J SchirrisPubMed 37154957
- Foods (Basel, Switzerland) 11:2022The Quality Changes and Proteomic Analysis of Cattle Muscle Postmortem during Rigor Mortis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenjiang Ding,Qichao Wei,Chunmei Liu,Hong Zhang,Feng HuangPubMed 35053949
- Disease models & mechanisms 14:2021TFAM-deficient mouse skin fibroblasts - an ex vivo model of mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManuel J Del Rey,Carolina Meroño,Cristina Municio,Alicia Usategui,María Mittelbrunn,Inés García-Consuegra,Gabriel Criado,José L PablosPubMed 34312668
- Nucleic acids research 48:12727-127452020Serine-70 phosphorylated Bcl-2 prevents oxidative stress-induced DNA damage by modulating the mitochondrial redox metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephen Jun Fei Chong,Kartini Iskandar,Jolin Xiao Hui Lai,Jianhua Qu,Deepika Raman,Rebecca Valentin,Charles Herbaux,Mary Collins,Ivan Cherh Chiet Low,Thomas Loh,Matthew Davids,Shazib PervaizPubMed 33245769
- The EMBO journal 39:e1039122020Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Lobo-Jarne et. al.PubMed 32511785
- Cells 9:2020Cytochrome Oxidase Subunit 4 Isoform Exchange Results in Modulation of Oxygen Affinity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Pajuelo Reguera et. al.PubMed 32075102
- Journal of diabetes research 2020:20571872020Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNA Gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Li et. al.PubMed 32083134
- The EMBO journal 39:e1028172020Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargherita Protasoni et. al.PubMed 31912925
- Disease models & mechanisms 12:2019Deterioration of mitochondrial bioenergetics and ultrastructure impairment in skeletal muscle of a transgenic minipig model in the early stages of Huntington's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie Rodinova et. al.PubMed 31278192
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Expected | Tested | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Cow | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Alternative names
Cytochrome c oxidase polypeptide Va, COX5A
Recommended products
Mouse Monoclonal COX5A antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, Cow samples. Cited in 54 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 6E9B12D5
- Light chain type
- kappa
- Concentration
- Purification notes
ab110262 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
COX5A also known as Cytochrome c oxidase subunit 5A plays a role in the electron transport chain which is essential in cellular respiration. This protein has a mass of about 17 kDa and is part of the mitochondrial inner membrane. COX5A is expressed in most tissues given its importance in energy production. It is homologous across species indicating evolutionary conservation and a fundamental biological role.
- Biological function summary
COX5A functions as a component of the cytochrome c oxidase complex also known as Complex IV in the mitochondrial electron transport chain. This complex catalyzes the reduction of oxygen to water facilitating ATP synthesis by oxidative phosphorylation. The assembly of cytochrome c oxidase involves several subunits and COX5A contributes to the structural integrity and regulation of this complex. It helps maintain efficient electron flow and energy conversion supporting high-energy demand processes within cells.
- Pathways
COX5A is an important player in the oxidative phosphorylation pathway. This pathway is critical for ATP production linking electron transfer with proton transport and chemiosmotic coupling. COX5A interacts with proteins like cytochrome c and other subunits of Complex IV to ensure proper function and energy conversion. The protein is also involved in cellular responses to hypoxia where it helps modulate oxygen utilization.
- Associated diseases and disorders
COX5A has connections to conditions related to impaired energy metabolism such as mitochondrial disorders like MELAS syndrome. Defects or insufficiencies in COX5A contribute to decreased mitochondrial function impacting tissues with high metabolic rates. The protein also relates to neurodegenerative diseases through its effect on mitochondrial stability and function. Its activity may influence disease progression by interacting with proteins that regulate mitochondrial dynamics such as others in the electron transport chain.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-COX5A antibody [6E9B12D5] (ab110262)
All lanes: Western blot - Anti-COX5A antibody [6E9B12D5] (ab110262) at 1 µg/mL
Lane 1: Human heart mitochondria at 5 µg
Lane 2: Bovine heart mitochondria at 1 µg
Lane 3: Rat heart mitochondria at 10 µg
Lane 4: Mouse heart mitochondria at 10 µg
Lane 5: HepG2 mitochondria at 20 µg
Predicted band size: 17 kDa
![Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262), expandable thumbnail](https://content.abcam.com/products/images/cox5a-antibody-6e9b12d5-ab110262--flow-cytometry-img75075.jpg "Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262)")
Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262)
HeLa cells were stained with 1 µg/mL ab110262 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
![Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262), expandable thumbnail](https://content.abcam.com/products/images/cox5a-antibody-6e9b12d5-ab110262--immunocytochemistry-immunofluorescence-img6430.gif "Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262)")
Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262)
Mitochondrial localization of COX5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110262 (10 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (1 µg/ml).
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