AB110262

Anti-COX5A antibody [6E9B12D5]

Name: Anti-COX5A antibody [6E9B12D5] (ab110262) | Abcam
Brand: Abcam Limited
SKU: AB110262
Price: 480 USD
Availability: InStock

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(61 Publications)

Mouse Monoclonal COX5A antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, Cow samples. Cited in 61 publications.

View Alternative Names

Cytochrome c oxidase polypeptide Va, COX5A

3 Images

Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (AB110262)

Flow Cyt

Unknown

Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (AB110262)

HeLa cells were stained with 1 µg/mL ab110262 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (AB110262)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (AB110262)

Mitochondrial localization of COX5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110262 (10 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (1 µg/ml).

Unknown

Western blot - Anti-COX5A antibody [6E9B12D5] (AB110262)

All lanes:

Western blot - Anti-COX5A antibody [6E9B12D5] (ab110262) at 1 µg/mL

Lane 1:

Human heart mitochondria at 5 µg

Lane 2:

Bovine heart mitochondria at 1 µg

Lane 3:

Rat heart mitochondria at 10 µg

Lane 4:

Mouse heart mitochondria at 10 µg

Lane 5:

HepG2 mitochondria at 20 µg

Predicted band size: 17 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6E9B12D5

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification notes

ab110262 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COX5A also known as Cytochrome c oxidase subunit 5A plays a role in the electron transport chain which is essential in cellular respiration. This protein has a mass of about 17 kDa and is part of the mitochondrial inner membrane. COX5A is expressed in most tissues given its importance in energy production. It is homologous across species indicating evolutionary conservation and a fundamental biological role.

Biological function summary

COX5A functions as a component of the cytochrome c oxidase complex also known as Complex IV in the mitochondrial electron transport chain. This complex catalyzes the reduction of oxygen to water facilitating ATP synthesis by oxidative phosphorylation. The assembly of cytochrome c oxidase involves several subunits and COX5A contributes to the structural integrity and regulation of this complex. It helps maintain efficient electron flow and energy conversion supporting high-energy demand processes within cells.

Pathways

COX5A is an important player in the oxidative phosphorylation pathway. This pathway is critical for ATP production linking electron transfer with proton transport and chemiosmotic coupling. COX5A interacts with proteins like cytochrome c and other subunits of Complex IV to ensure proper function and energy conversion. The protein is also involved in cellular responses to hypoxia where it helps modulate oxygen utilization.

COX5A has connections to conditions related to impaired energy metabolism such as mitochondrial disorders like MELAS syndrome. Defects or insufficiencies in COX5A contribute to decreased mitochondrial function impacting tissues with high metabolic rates. The protein also relates to neurodegenerative diseases through its effect on mitochondrial stability and function. Its activity may influence disease progression by interacting with proteins that regulate mitochondrial dynamics such as others in the electron transport chain.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information Cytochrome c oxidase subunit 5A, mitochondrial

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Publications (61)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1422693 PubMed39611170

2024

Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Weifen Li,Shengnan Mou,Tahir Ali,Tianxiang Li,Yan Liu,Shupeng Li,Xiaoming Yu,Zhi-Jian Yu

Nature communications 15:7730 PubMed39231983

2024

Cannabidiol ameliorates mitochondrial disease via PPARγ activation in preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Puighermanal,Marta Luna-Sánchez,Alejandro Gella,Gunter van der Walt,Andrea Urpi,María Royo,Paula Tena-Morraja,Isabella Appiah,Maria Helena de Donato,Fabien Menardy,Patrizia Bianchi,Anna Esteve-Codina,Laura Rodríguez-Pascau,Cristina Vergara,Mercè Gómez-Pallarès,Giovanni Marsicano,Luigi Bellocchio,Marc Martinell,Elisenda Sanz,Sandra Jurado,Francesc Xavier Soriano,Pilar Pizcueta,Albert Quintana

iScience 27:110560 PubMed39184436

2024

Mitochondrial translation is the primary determinant of secondary mitochondrial complex I deficiencies.

Applications

Unspecified application

Species

Unspecified reactive species

Kristýna Čunátová,Marek Vrbacký,Guillermo Puertas-Frias,Lukáš Alán,Marie Vanišová,María José Saucedo-Rodríguez,Josef Houštěk,Erika Fernández-Vizarra,Jiří Neužil,Alena Pecinová,Petr Pecina,Tomáš Mráček

PloS one 18:e0291442 PubMed37725617

2023

Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Serrano-Lorenzo,Dino Gobelli,Rocío Garrido-Moraga,María J Esteban-Amo,José R López-López,Antonio Orduña,Miguel A de la Fuente,Miguel A Martín,María Simarro

Archives of toxicology 97:1927-1941 PubMed37154957

2023

Metabolic impact of genetic and chemical ADP/ATP carrier inhibition in renal proximal tubule epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte A Hoogstraten,Maaike M E Jacobs,Guido de Boer,Melissa A E van de Wal,Werner J H Koopman,Jan A M Smeitink,Frans G M Russel,Tom J J Schirris

Foods (Basel, Switzerland) 11: PubMed35053949

2022

The Quality Changes and Proteomic Analysis of Cattle Muscle Postmortem during Rigor Mortis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenjiang Ding,Qichao Wei,Chunmei Liu,Hong Zhang,Feng Huang

Disease models & mechanisms 14: PubMed34312668

2021

TFAM-deficient mouse skin fibroblasts - an ex vivo model of mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel J Del Rey,Carolina Meroño,Cristina Municio,Alicia Usategui,María Mittelbrunn,Inés García-Consuegra,Gabriel Criado,José L Pablos

The Journal of biological chemistry 297:101101 PubMed34419449

2021

Retinoic acid exerts sexually dimorphic effects on muscle energy metabolism and function.

Applications

Unspecified application

Species

Unspecified reactive species

Yaxin Zhao,Marta Vuckovic,Hong Sik Yoo,Nina Fox,Adrienne Rodriguez,Kyler McKessy,Joseph L Napoli

Nucleic acids research 48:12727-12745 PubMed33245769

2020

Serine-70 phosphorylated Bcl-2 prevents oxidative stress-induced DNA damage by modulating the mitochondrial redox metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen Jun Fei Chong,Kartini Iskandar,Jolin Xiao Hui Lai,Jianhua Qu,Deepika Raman,Rebecca Valentin,Charles Herbaux,Mary Collins,Ivan Cherh Chiet Low,Thomas Loh,Matthew Davids,Shazib Pervaiz

The EMBO journal 39:e103912 PubMed32511785

2020

Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Lobo-Jarne,Rafael Pérez-Pérez,Flavia Fontanesi,Alba Timón-Gómez,Ilka Wittig,Ana Peñas,Pablo Serrano-Lorenzo,Inés García-Consuegra,Joaquín Arenas,Miguel A Martín,Antoni Barrientos,Cristina Ugalde

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COX5A antibody [6E9B12D5]

Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (AB110262)

Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (AB110262)

Western blot - Anti-COX5A antibody [6E9B12D5] (AB110262)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (61)

Product promise