AB250442

Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal COX5A antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Cytochrome c oxidase polypeptide Va, COX5A

6 Images

Flow Cytometry (Intracellular) - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

This data was developed using ab181226, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling COX5A with ab181226 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

This data was developed using ab181226, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling COX5A with ab181226 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

This data was developed using ab181226, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COX5A with ab181226 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

This data was developed using ab181226, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone-fixed HeLa cells labeling COX5A with ab181226 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

Supplier Data

Immunoprecipitation - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

This data was developed using ab181226, the same antibody clone in a different buffer formulation.

Western blot analysis of HeLa cell lysate immunoprecipitated with ab181226 at 1/50 dilution.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-COX5A antibody [EPR14207(B)] (<a href='/en-us/products/primary-antibodies/cox5a-antibody-epr14207b-ab181226'>ab181226</a>)

Predicted band size: 17 kDa

Observed band size: 13 kDa

false

Supplier Data

Western blot - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

This data was developed using ab181226, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-COX5A antibody [EPR14207(B)] (<a href='/en-us/products/primary-antibodies/cox5a-antibody-epr14207b-ab181226'>ab181226</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 13 kDa

false

Unconjugated

Anti-COX5A antibody [EPR14207(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14207(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250442 is the carrier-free version of ab181226.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COX5A also known as Cytochrome c oxidase subunit 5A plays a role in the electron transport chain which is essential in cellular respiration. This protein has a mass of about 17 kDa and is part of the mitochondrial inner membrane. COX5A is expressed in most tissues given its importance in energy production. It is homologous across species indicating evolutionary conservation and a fundamental biological role.

Biological function summary

COX5A functions as a component of the cytochrome c oxidase complex also known as Complex IV in the mitochondrial electron transport chain. This complex catalyzes the reduction of oxygen to water facilitating ATP synthesis by oxidative phosphorylation. The assembly of cytochrome c oxidase involves several subunits and COX5A contributes to the structural integrity and regulation of this complex. It helps maintain efficient electron flow and energy conversion supporting high-energy demand processes within cells.

Pathways

COX5A is an important player in the oxidative phosphorylation pathway. This pathway is critical for ATP production linking electron transfer with proton transport and chemiosmotic coupling. COX5A interacts with proteins like cytochrome c and other subunits of Complex IV to ensure proper function and energy conversion. The protein is also involved in cellular responses to hypoxia where it helps modulate oxygen utilization.

COX5A has connections to conditions related to impaired energy metabolism such as mitochondrial disorders like MELAS syndrome. Defects or insufficiencies in COX5A contribute to decreased mitochondrial function impacting tissues with high metabolic rates. The protein also relates to neurodegenerative diseases through its effect on mitochondrial stability and function. Its activity may influence disease progression by interacting with proteins that regulate mitochondrial dynamics such as others in the electron transport chain.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information Cytochrome c oxidase subunit 5A, mitochondrial

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

Immunoprecipitation - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

Western blot - Anti-COX5A antibody [EPR14207(B)] - BSA and Azide free (AB250442)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise