Anti-COX5B antibody

• IP

Supplier Data

Immunoprecipitation - Anti-COX5B antibody (AB264401)

COX5B was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264401 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab264401 at 0.4 μg/ml.

Lane 1 : ab264401 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-COX5B antibody (ab264401)

Predicted band size: 14 kDa

false

• WB

Supplier Data

Western blot - Anti-COX5B antibody (AB264401)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-COX5B antibody (ab264401) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 14 kDa

true

Exposure time: 3min