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Mouse Monoclonal COX6A1 antibody. Suitable for Flow Cyt, WB and reacts with Human, Rat, Mouse samples. Cited in 3 publications.


Images

Flow Cytometry - Anti-COX6A1 antibody [14A3AD2BH4] (AB110265), expandable thumbnail
  • Western blot - Anti-COX6A1 antibody [14A3AD2BH4] (AB110265), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
Cow
Predicted
Predicted
Dog
Predicted
Predicted
Rabbit
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rabbit, Cow, Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Species
Rat
Dilution info
5 µg/mL
Notes

-

Species
Mouse
Dilution info
5 µg/mL
Notes

-

Predicted
Predicted

Species
Rabbit, Cow, Dog
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit SSG

Recommended products

Mouse Monoclonal COX6A1 antibody. Suitable for Flow Cyt, WB and reacts with Human, Rat, Mouse samples. Cited in 3 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
14A3AD2BH4
Specificity

ab110265 recognises the Liver isoform in human, rat, mouse.

Light chain type
kappa
Concentration
Loading...
Purification notes

ab110265 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

COX6A1 also known as Cytochrome c oxidase subunit 6A1 plays an important role in the electron transport chain by being part of the cytochrome c oxidase (complex IV) in mitochondria. This subunit has a mass of approximately 14 kDa. COX6A1 expresses in various human tissues but shows higher expression in tissues with significant energy demands such as the heart and skeletal muscle. This evidence suggests its critical involvement in cellular energy production.

Biological function summary

COX6A1 contributes to the formation of the cytochrome c oxidase complex which is the terminal enzyme in the mitochondrial respiratory chain. This complex facilitates the transfer of electrons from cytochrome c to oxygen leading to ATP production through oxidative phosphorylation. COX6A1 is an integral component of this complex which exists as part of the inner mitochondrial membrane. It interacts with other subunits to ensure efficient electron transfer and energy conversion.

Pathways

COX6A1 heavily influences the mitochondrial respiratory pathway particularly in oxidative phosphorylation. This pathway includes important components like NADH: ubiquinone oxidoreductase and cytochrome c. COX6A1 bridges interactions within the complex IV with proteins such as COX1 enabling proper electron transport and proton pumping necessary for ATP synthesis. Dysfunction in these interactions may affect overall cellular respiration efficiency.

Associated diseases and disorders

Mutations and dysregulation of COX6A1 relate to various mitochondrial disorders and neurodegenerative conditions such as Leigh syndrome. Its interactions with other mitochondrial proteins like COX2 and COX3 are vital for maintaining mitochondrial function. Deficiencies in COX6A1 can disrupt proper electron transport leading to decreased ATP production and contributing to pathology in energy-demanding tissues like the brain and muscles.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Anti-COX6A1 antibody [14A3AD2BH4] (ab110265), expandable thumbnail

    Flow Cytometry - Anti-COX6A1 antibody [14A3AD2BH4] (ab110265)

    Overlay histogram showing HepG2 cells stained with ab110265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110265, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-COX6A1 antibody [14A3AD2BH4] (ab110265), expandable thumbnail

    Western blot - Anti-COX6A1 antibody [14A3AD2BH4] (ab110265)

    Extra bands in the mouse sample (lane 3) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

    All lanes: Western blot - Anti-COX6A1 antibody [14A3AD2BH4] (ab110265) at 5 µg/mL

    Lane 1: Human Heart Lysate

    Lane 2: Rat Heart Lysate

    Lane 3: Mouse Heart Lysate

    Lane 4: HepG2 cell lysate

    Predicted band size: 12 kDa

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Product protocols

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