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AB110264

Anti-COX6A2 antibody [4H2A5]

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(2 Publications)

Mouse Monoclonal COX6A2 antibody. Suitable for WB and reacts with Cow samples. Cited in 2 publications.

View Alternative Names

COX6A, COX6A2, Cytochrome c oxidase polypeptide VIa-heart, Cytochrome c oxidase polypeptide VIb, COXVIAH

1 Images
Western blot - Anti-COX6A2 antibody [4H2A5] (AB110264)
  • WB

Unknown

Western blot - Anti-COX6A2 antibody [4H2A5] (AB110264)

All lanes:

Western blot - Anti-COX6A2 antibody [4H2A5] (ab110264) at 25 µg/mL

All lanes:

Isolated mitochondria from bovine heart at 1 µg

Predicted band size: 11 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4H2A5

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Cow

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "25 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110264 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

`COX6A2` also known as cytochrome c oxidase subunit 6A2 is a component of the cytochrome c oxidase complex which is part of the electron transport chain. This protein weighs approximately 9 kDa. It is mainly expressed in muscle tissues both cardiac and skeletal reflecting its significant role in tissues with high energy demands. The `COX6A2` protein contributes to the proper functioning of cytochrome c oxidase aiding in the efficient transfer of electrons from cytochrome c to oxygen the final electron acceptor in the chain.
Biological function summary

`COX6A2` participates in energy production within cells by facilitating mitochondrial oxidative phosphorylation. It is part of the cytochrome c oxidase complex which consists of multiple subunits each playing a unique role in electron transport and proton pumping to generate a proton gradient across the mitochondrial membrane. This proton gradient is essential for ATP synthesis. The activity of `COX6A2` supports normal mitochondrial function and energy homeostasis especially in cells with heavy metabolic requirements.

Pathways

The function of `COX6A2` integrates into the mitochondrial respiratory chain specifically in the oxidative phosphorylation pathway. This pathway is important for ATP generation an important process for cellular energy supplies. Additionally `COX6A2` is associated with the regulation of the apoptosis pathway as disruptions in the respiratory chain can trigger programmed cell death. The function of cytochrome c and subunit interactions underline these pathways with each having pivotal roles in maintaining cellular metabolism and survival.

Disruptions in `COX6A2` activity relate to mitochondrial diseases such as cytochrome c oxidase deficiency which can lead to severe metabolic complications. This deficiency affects energy production in muscles and other tissues by impairing the electron transport chain. Additionally mutations or malfunctions involving `COX6A2` may have links to cardiac disorders due to its strong expression in cardiac muscle. The alteration in `COX6A2` can impact the function of related proteins such as those included in the mitochondrial respiratory chain ultimately affecting energy-dependent tissues and processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. Plays a role in the assembly and stabilization of complex IV (By similarity).
See full target information COX6A2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 32:1294-1302 PubMed39962244

2025

Distinct developmental outcomes in DNA repair-deficient FANCC c.67delG mutant and FANCC Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Swarna Beesetti,Cliff Guy,Shyam Sirasanagandla,Mao Yang,Rhea Jr Sumpter,Heather Sheppard,Stephane Pelletier,Marcin W Wlodarski,Douglas R Green

Meat science 199:109126 PubMed36736126

2023

Integrating identification and targeted proteomics to discover the potential indicators of postmortem lamb meat quality.

Applications

Unspecified application

Species

Unspecified reactive species

Caiyan Huang,Christophe Blecker,Li Chen,Can Xiang,Xiaochun Zheng,Zhenyu Wang,Dequan Zhang
View all publications
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