Rabbit Polyclonal COX6A2 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human COX6A2 aa 1 to C-terminus.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human COX6A2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q02221
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
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Target data
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. Plays a role in the assembly and stabilization of complex IV (PubMed:31155743).
Alternative names
COX6A, COX6AH, COX6A2, Cytochrome c oxidase polypeptide VIa-heart, Cytochrome c oxidase subunit VIA-muscle, COXVIAH, COX VIa-M
Recommended products
Rabbit Polyclonal COX6A2 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human COX6A2 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human COX6A2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q02221
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
`COX6A2` also known as cytochrome c oxidase subunit 6A2 is a component of the cytochrome c oxidase complex which is part of the electron transport chain. This protein weighs approximately 9 kDa. It is mainly expressed in muscle tissues both cardiac and skeletal reflecting its significant role in tissues with high energy demands. The `COX6A2` protein contributes to the proper functioning of cytochrome c oxidase aiding in the efficient transfer of electrons from cytochrome c to oxygen the final electron acceptor in the chain.
- Biological function summary
`COX6A2` participates in energy production within cells by facilitating mitochondrial oxidative phosphorylation. It is part of the cytochrome c oxidase complex which consists of multiple subunits each playing a unique role in electron transport and proton pumping to generate a proton gradient across the mitochondrial membrane. This proton gradient is essential for ATP synthesis. The activity of `COX6A2` supports normal mitochondrial function and energy homeostasis especially in cells with heavy metabolic requirements.
- Pathways
The function of `COX6A2` integrates into the mitochondrial respiratory chain specifically in the oxidative phosphorylation pathway. This pathway is important for ATP generation an important process for cellular energy supplies. Additionally `COX6A2` is associated with the regulation of the apoptosis pathway as disruptions in the respiratory chain can trigger programmed cell death. The function of cytochrome c and subunit interactions underline these pathways with each having pivotal roles in maintaining cellular metabolism and survival.
- Associated diseases and disorders
Disruptions in `COX6A2` activity relate to mitochondrial diseases such as cytochrome c oxidase deficiency which can lead to severe metabolic complications. This deficiency affects energy production in muscles and other tissues by impairing the electron transport chain. Additionally mutations or malfunctions involving `COX6A2` may have links to cardiac disorders due to its strong expression in cardiac muscle. The alteration in `COX6A2` can impact the function of related proteins such as those included in the mitochondrial respiratory chain ultimately affecting energy-dependent tissues and processes.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-COX6A2 antibody (ab96096)
12% SDS-PAGE
All lanes: Western blot - Anti-COX6A2 antibody (ab96096) at 1/500 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 11 kDa
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