JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB96096

Anti-COX6A2 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal COX6A2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human COX6A2 aa 1 to C-terminus.

View Alternative Names

COX6A, COX6AH, COX6A2, Cytochrome c oxidase polypeptide VIa-heart, Cytochrome c oxidase subunit VIA-muscle, COXVIAH, COX VIa-M

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-COX6A2 antibody (AB96096)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX6A2 antibody (AB96096)

Immunofluorescence analysis of SK-N-SH cells fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min labelling COX6A2 protein at mitochondria using ab96096 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6A2 antibody (AB96096)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6A2 antibody (AB96096)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue labelling COX6A2 protein at mitochondria with ab96096 at a 1/500 dilution.
Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

Western blot - Anti-COX6A2 antibody (AB96096)
  • WB

Unknown

Western blot - Anti-COX6A2 antibody (AB96096)

12% SDS-PAGE

All lanes:

Western blot - Anti-COX6A2 antibody (ab96096) at 1/500 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human COX6A2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02221

style
left-column

Alternative Products

Primary Antibodies

AB110264

Anti-COX6A2 antibody [4H2A5]

Western blot - Anti-COX6A2 antibody [4H2A5] (AB110264)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB131143

Anti-COX7A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7A2 antibody (AB131143)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB110265

Anti-COX6A1 antibody [14A3AD2BH4]

Flow Cytometry - Anti-COX6A1 antibody [14A3AD2BH4] (AB110265)

  • 4
  • 1
Sample Prep & Detection Kits

AB110168

Mitochondria Isolation Kit for Tissue

Western blot - Mitochondria Isolation Kit for Tissue (AB110168)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

`COX6A2` also known as cytochrome c oxidase subunit 6A2 is a component of the cytochrome c oxidase complex which is part of the electron transport chain. This protein weighs approximately 9 kDa. It is mainly expressed in muscle tissues both cardiac and skeletal reflecting its significant role in tissues with high energy demands. The `COX6A2` protein contributes to the proper functioning of cytochrome c oxidase aiding in the efficient transfer of electrons from cytochrome c to oxygen the final electron acceptor in the chain.
Biological function summary

`COX6A2` participates in energy production within cells by facilitating mitochondrial oxidative phosphorylation. It is part of the cytochrome c oxidase complex which consists of multiple subunits each playing a unique role in electron transport and proton pumping to generate a proton gradient across the mitochondrial membrane. This proton gradient is essential for ATP synthesis. The activity of `COX6A2` supports normal mitochondrial function and energy homeostasis especially in cells with heavy metabolic requirements.

Pathways

The function of `COX6A2` integrates into the mitochondrial respiratory chain specifically in the oxidative phosphorylation pathway. This pathway is important for ATP generation an important process for cellular energy supplies. Additionally `COX6A2` is associated with the regulation of the apoptosis pathway as disruptions in the respiratory chain can trigger programmed cell death. The function of cytochrome c and subunit interactions underline these pathways with each having pivotal roles in maintaining cellular metabolism and survival.

Disruptions in `COX6A2` activity relate to mitochondrial diseases such as cytochrome c oxidase deficiency which can lead to severe metabolic complications. This deficiency affects energy production in muscles and other tissues by impairing the electron transport chain. Additionally mutations or malfunctions involving `COX6A2` may have links to cardiac disorders due to its strong expression in cardiac muscle. The alteration in `COX6A2` can impact the function of related proteins such as those included in the mitochondrial respiratory chain ultimately affecting energy-dependent tissues and processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. Plays a role in the assembly and stabilization of complex IV (PubMed : 31155743).
See full target information COX6A2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes research 2018:9257874 PubMed30276217

2018

Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study.

Applications

Unspecified application

Species

Unspecified reactive species

Steen Larsen,Andreas Vigelsø,Sune Dandanell,Clara Prats,Flemming Dela,Jørn Wulff Helge
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com