Mouse Monoclonal COX6B1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Cow, Human samples. Cited in 12 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (AB110266), expandable thumbnail](https://content.abcam.com/products/images/cox6b1-antibody-3f9d3d11af6-ab110266--immunocytochemistry-immunofluorescence-img111288.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (AB110266)")
![Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (AB110266), expandable thumbnail](https://content.abcam.com/products/images/cox6b1-antibody-3f9d3d11af6-ab110266--western-blot-img7111.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (AB110266)")
Publications
Filter by
- Development (Cambridge, England) 147:2020Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnes Cheong et. al.PubMed 32376682
- The EMBO journal 39:e1028172020Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargherita Protasoni et. al.PubMed 31912925
- EMBO molecular medicine 11:2019Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarthik Mohanraj,Michal Wasilewski,Cristiane Benincá,Dominik Cysewski,Jaroslaw Poznanski,Paulina Sakowska,Zaneta Bugajska,Markus Deckers,Sven Dennerlein,Erika Fernandez-Vizarra,Peter Rehling,Michal Dadlez,Massimo Zeviani,Agnieszka ChacinskaPubMed 30885959
- EMBO molecular medicine 11:2018APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlba Signes,Raffaele Cerutti,Anna S Dickson,Cristiane Benincá,Elizabeth C Hinchy,Daniele Ghezzi,Rosalba Carrozzo,Enrico Bertini,Michael P Murphy,James A Nathan,Carlo Viscomi,Erika Fernandez-Vizarra,Massimo ZevianiPubMed 30552096
- Age (Dordrecht, Netherlands) 37:97872015Upregulation of cytochrome c oxidase subunit 6b1 (Cox6b1) and formation of mitochondrial supercomplexes: implication of Cox6b1 in the effect of calorie restriction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSang-Eun Kim et. al.PubMed 25929654
- PloS one 9:e901372014Dual mode action of mangiferin in mouse liver under high fat diet.
- Human molecular genetics 23:2901-132014Human COX20 cooperates with SCO1 and SCO2 to mature COX2 and promote the assembly of cytochrome c oxidase.
- Nature 483:608-122012Deregulated MYC expression induces dependence upon AMPK-related kinase 5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLidan Liu et. al.PubMed 22460906
- American journal of physiology. Heart and circulatory physiology 298:H75-912009Cardioprotection leads to novel changes in the mitochondrial proteome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenee Wong,Angel M Aponte,Charles Steenbergen,Elizabeth MurphyPubMed 19855063
- American journal of physiology. Heart and circulatory physiology 294:H2637-452008Differential loss of cytochrome-c oxidase subunits in ischemia-reperfusion injury: exacerbation of COI subunit loss by PKC-epsilon inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQilin Yu,Tiffany Nguyen,Mourad Ogbi,Robert W Caldwell,John A JohnsonPubMed 18408135
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Cow | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Cow | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Alternative names
COX6B, COX6B1, Cytochrome c oxidase subunit 6B1, Cytochrome c oxidase subunit VIb isoform 1, COX VIb-1
Recommended products
Mouse Monoclonal COX6B1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Cow, Human samples. Cited in 12 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 3F9D3D11AF6
- Light chain type
- kappa
- Concentration
- Purification notes
Near homogeneity as judged by SDS-PAGE. ab110266 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
COX6B1 also called Cytochrome c oxidase subunit 6B1 is an integral part of the cytochrome c oxidase (complex IV) of the mitochondrial electron transport chain. This protein weighs approximately 11 kDa. It is expressed in various tissues with higher expression in heart and muscle tissues. COX6B1 acts as a connecting unit between two core components of the cytochrome c oxidase secure the assembly and stability of the complex.
- Biological function summary
The COX6B1 protein plays an important role in the electron transport chain by facilitating the transfer of electrons from cytochrome c to oxygen. It helps in efficient energy production by contributing to the proton gradient used in ATP synthesis. As part of the cytochrome c oxidase complex COX6B1 works with other subunits to enable cellular respiration. Its stability is important for maintaining the proper function and formation of the cytochrome c oxidase complex.
- Pathways
The electron transport chain is the primary pathway where COX6B1 operates. It is involved in oxidative phosphorylation a critical process for energy production in cells. Through this pathway COX6B1 interacts with other proteins such as cytochrome c and other subunits of cytochrome c oxidase like COX2 and COX4 which are part of the same complex. These interactions ensure the efficiency of ATP generation from ADP.
- Associated diseases and disorders
Mutations or dysfunctions in the COX6B1 gene can lead to mitochondrial conditions such as Complex IV deficiency and Leigh syndrome. These conditions often result in energy production deficits and neurological impairment. COX6B1 is linked to other proteins like COX4 which when mutated can also contribute to similar mitochondrial disorders. Identifying changes in COX6B1 can provide insights into mitochondrial pathologies and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266), expandable thumbnail](https://content.abcam.com/products/images/cox6b1-antibody-3f9d3d11af6-ab110266--immunocytochemistry-immunofluorescence-img111288.jpg "Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)")
Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
![Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266), expandable thumbnail](https://content.abcam.com/products/images/cox6b1-antibody-3f9d3d11af6-ab110266--western-blot-img7111.jpg "Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)")
Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
All lanes: Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266) at 1 µg/mL
Lane 1: Isolated mitochondria from Human Heart at 5 µg
Lane 2: Isolated mitochondria from Bovine Heart at 4 µg
Lane 3: Isolated mitochondria from Rat Heart at 10 µg
Lane 4: Isolated mitochondria from Mouse Heart at 10 µg
Predicted band size: 10 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com