AB110266

Anti-COX6B1 antibody [3F9D3D11AF6]

Name: Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266) | Abcam
Brand: Abcam Limited
SKU: AB110266

Be the first to review this product! Submit a review

(15 Publications)

Mouse Monoclonal COX6B1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Cow, Human samples. Cited in 15 publications.

View Alternative Names

COX6B, COX6B1, Cytochrome c oxidase subunit 6B1, Cytochrome c oxidase subunit VIb isoform 1, COX VIb-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (AB110266)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (AB110266)

ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Unknown

Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (AB110266)

All lanes:

Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266) at 1 µg/mL

Lane 1:

Isolated mitochondria from Human Heart at 5 µg

Lane 2:

Isolated mitochondria from Bovine Heart at 4 µg

Lane 3:

Isolated mitochondria from Rat Heart at 10 µg

Lane 4:

Isolated mitochondria from Mouse Heart at 10 µg

Predicted band size: 10 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3F9D3D11AF6

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification notes

Near homogeneity as judged by SDS-PAGE. ab110266 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COX6B1 also called Cytochrome c oxidase subunit 6B1 is an integral part of the cytochrome c oxidase (complex IV) of the mitochondrial electron transport chain. This protein weighs approximately 11 kDa. It is expressed in various tissues with higher expression in heart and muscle tissues. COX6B1 acts as a connecting unit between two core components of the cytochrome c oxidase secure the assembly and stability of the complex.

Biological function summary

The COX6B1 protein plays an important role in the electron transport chain by facilitating the transfer of electrons from cytochrome c to oxygen. It helps in efficient energy production by contributing to the proton gradient used in ATP synthesis. As part of the cytochrome c oxidase complex COX6B1 works with other subunits to enable cellular respiration. Its stability is important for maintaining the proper function and formation of the cytochrome c oxidase complex.

Pathways

The electron transport chain is the primary pathway where COX6B1 operates. It is involved in oxidative phosphorylation a critical process for energy production in cells. Through this pathway COX6B1 interacts with other proteins such as cytochrome c and other subunits of cytochrome c oxidase like COX2 and COX4 which are part of the same complex. These interactions ensure the efficiency of ATP generation from ADP.

Mutations or dysfunctions in the COX6B1 gene can lead to mitochondrial conditions such as Complex IV deficiency and Leigh syndrome. These conditions often result in energy production deficits and neurological impairment. COX6B1 is linked to other proteins like COX4 which when mutated can also contribute to similar mitochondrial disorders. Identifying changes in COX6B1 can provide insights into mitochondrial pathologies and potential therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information COX6B1

style

left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2404620 PubMed39716856

2024

Nuclear Control of Mitochondrial Homeostasis and Venetoclax Efficacy in AML via COX4I1.

Applications

Unspecified application

Species

Unspecified reactive species

Leisi Zhang,Honghai Zhang,Ting-Yu Wang,Mingli Li,Anthony K N Chan,Hyunjun Kang,Lai C Foong,Qiao Liu,Sheela Pangeni Pokharel,Nicole M Mattson,Priyanka Singh,Zeinab Elsayed,Benjamin Kuang,Xueer Wang,Steven T Rosen,Jianjun Chen,Lu Yang,Tsui-Fen Chou,Rui Su,Chun-Wei David Chen

Metabolism: clinical and experimental 114:154416 PubMed33137378

2020

Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Vanessa Fiorentino,Adriana Monroy,Subash Kamath,Rosa Sotero,Michele Dei Cas,Giuseppe Daniele,Alberto O Chavez,Muhammad Abdul-Ghani,Marta Letizia Hribal,Giorgio Sesti,Devjit Tripathy,Ralph A DeFronzo,Franco Folli

Development (Cambridge, England) 147: PubMed32376682

2020

Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes Cheong,Danielle Archambault,Rinat Degani,Elizabeth Iverson,Kimberly D Tremblay,Jesse Mager

The EMBO journal 39:e102817 PubMed31912925

2020

Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.

Applications

Unspecified application

Species

Unspecified reactive species

Margherita Protasoni,Rafael Pérez-Pérez,Teresa Lobo-Jarne,Michael E Harbour,Shujing Ding,Ana Peñas,Francisca Diaz,Carlos T Moraes,Ian M Fearnley,Massimo Zeviani,Cristina Ugalde,Erika Fernández-Vizarra

EMBO molecular medicine 11: PubMed30885959

2019

Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7.

Applications

Unspecified application

Species

Unspecified reactive species

Karthik Mohanraj,Michal Wasilewski,Cristiane Benincá,Dominik Cysewski,Jaroslaw Poznanski,Paulina Sakowska,Zaneta Bugajska,Markus Deckers,Sven Dennerlein,Erika Fernandez-Vizarra,Peter Rehling,Michal Dadlez,Massimo Zeviani,Agnieszka Chacinska

EMBO molecular medicine 11: PubMed30552096

2018

APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Signes,Raffaele Cerutti,Anna S Dickson,Cristiane Benincá,Elizabeth C Hinchy,Daniele Ghezzi,Rosalba Carrozzo,Enrico Bertini,Michael P Murphy,James A Nathan,Carlo Viscomi,Erika Fernandez-Vizarra,Massimo Zeviani

EMBO reports 18:477-494 PubMed28082314

2017

A -knockout reveals translation-independent control of human mitochondrial complex IV biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Myriam Bourens,Antoni Barrientos

Age (Dordrecht, Netherlands) 37:9787 PubMed25929654

2015

Upregulation of cytochrome c oxidase subunit 6b1 (Cox6b1) and formation of mitochondrial supercomplexes: implication of Cox6b1 in the effect of calorie restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Eun Kim,Ryoichi Mori,Toshimitsu Komatsu,Takuya Chiba,Hiroko Hayashi,Seongjoon Park,Michiru D Sugawa,Norbert A Dencher,Isao Shimokawa

PloS one 9:e90137 PubMed24598864

2014

Dual mode action of mangiferin in mouse liver under high fat diet.

Applications

Species

Mouse

Jihyeon Lim,Zhongbo Liu,Pasha Apontes,Daorong Feng,Jeffrey E Pessin,Anthony A Sauve,Ruth H Angeletti,Yuling Chi

Human molecular genetics 23:2901-13 PubMed24403053

2014

Human COX20 cooperates with SCO1 and SCO2 to mature COX2 and promote the assembly of cytochrome c oxidase.

Applications

Unspecified application

Species

Human

Myriam Bourens,Aren Boulet,Scot C Leary,Antoni Barrientos

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COX6B1 antibody [3F9D3D11AF6]

Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (AB110266)

Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (AB110266)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise