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AB131277

Anti-COX6B1 antibody [EPR7647]

4

(1 Review)

|

(4 Publications)

Rabbit Recombinant Monoclonal COX6B1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

COX6B, COX6B1, Cytochrome c oxidase subunit 6B1, Cytochrome c oxidase subunit VIb isoform 1, COX VIb-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] (AB131277)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] (AB131277)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling COX6B1 with ab131277 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [EPR7647] (AB131277)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [EPR7647] (AB131277)

Immunofluorescent analysis of HepG2 cells labelling COX6B1 with ab131277 at 1/50 dilution.

Western blot - Anti-COX6B1 antibody [EPR7647] (AB131277)
  • WB

Unknown

Western blot - Anti-COX6B1 antibody [EPR7647] (AB131277)

All lanes:

Western blot - Anti-COX6B1 antibody [EPR7647] (ab131277) at 1/1000 dilution

Lane 1:

HL60 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa

false

OI-RD Scanning - Anti-COX6B1 antibody [EPR7647] (AB131277)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-COX6B1 antibody [EPR7647] (AB131277)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-COX6B1 antibody [EPR7647] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7647

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COX6B1 also called Cytochrome c oxidase subunit 6B1 is an integral part of the cytochrome c oxidase (complex IV) of the mitochondrial electron transport chain. This protein weighs approximately 11 kDa. It is expressed in various tissues with higher expression in heart and muscle tissues. COX6B1 acts as a connecting unit between two core components of the cytochrome c oxidase secure the assembly and stability of the complex.
Biological function summary

The COX6B1 protein plays an important role in the electron transport chain by facilitating the transfer of electrons from cytochrome c to oxygen. It helps in efficient energy production by contributing to the proton gradient used in ATP synthesis. As part of the cytochrome c oxidase complex COX6B1 works with other subunits to enable cellular respiration. Its stability is important for maintaining the proper function and formation of the cytochrome c oxidase complex.

Pathways

The electron transport chain is the primary pathway where COX6B1 operates. It is involved in oxidative phosphorylation a critical process for energy production in cells. Through this pathway COX6B1 interacts with other proteins such as cytochrome c and other subunits of cytochrome c oxidase like COX2 and COX4 which are part of the same complex. These interactions ensure the efficiency of ATP generation from ADP.

Mutations or dysfunctions in the COX6B1 gene can lead to mitochondrial conditions such as Complex IV deficiency and Leigh syndrome. These conditions often result in energy production deficits and neurological impairment. COX6B1 is linked to other proteins like COX4 which when mutated can also contribute to similar mitochondrial disorders. Identifying changes in COX6B1 can provide insights into mitochondrial pathologies and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information COX6B1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan

Bioscience reports 44: PubMed38419527

2024

Integrative analysis of metabolism subtypes and identification of prognostic metabolism-related genes for glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Li,Yutian Wei,Jiali Liu,Shupeng Cheng,Xia Zhang,Huaide Qiu,Jianan Li,Chuan He

RNA (New York, N.Y.) 30:223-239 PubMed38164626

2024

LARP4 is an RNA-binding protein that binds nuclear-encoded mitochondrial mRNAs to promote mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin M Lewis,Chae Yun Cho,Hsuan-Lin Her,Orel Mizrahi,Tony Hunter,Gene W Yeo

International journal of oncology 57:1293-1306 PubMed33174046

2020

Glioma cells are resistant to inflammation‑induced alterations of mitochondrial dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Wange Fan,Yanan Song,Zongyao Ren,Xiaoli Cheng,Pu Li,Huiling Song,Liyun Jia
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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