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Rabbit Recombinant Monoclonal COX6B1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (AB248401), expandable thumbnail
  • Western blot - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (AB248401), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (AB248401), expandable thumbnail
  • OI-RD Scanning - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (AB248401), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow CytWB
Human
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

COX6B, COX6B1, Cytochrome c oxidase subunit 6B1, Cytochrome c oxidase subunit VIb isoform 1, COX VIb-1

Recommended products

Rabbit Recombinant Monoclonal COX6B1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7647
Purification technique
Affinity purification Protein A
Dissociation constant
2.49 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248401 is the carrier-free version of Anti-COX6B1 antibody [EPR7647] ab131277.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

COX6B1 also called Cytochrome c oxidase subunit 6B1 is an integral part of the cytochrome c oxidase (complex IV) of the mitochondrial electron transport chain. This protein weighs approximately 11 kDa. It is expressed in various tissues with higher expression in heart and muscle tissues. COX6B1 acts as a connecting unit between two core components of the cytochrome c oxidase secure the assembly and stability of the complex.

Biological function summary

The COX6B1 protein plays an important role in the electron transport chain by facilitating the transfer of electrons from cytochrome c to oxygen. It helps in efficient energy production by contributing to the proton gradient used in ATP synthesis. As part of the cytochrome c oxidase complex COX6B1 works with other subunits to enable cellular respiration. Its stability is important for maintaining the proper function and formation of the cytochrome c oxidase complex.

Pathways

The electron transport chain is the primary pathway where COX6B1 operates. It is involved in oxidative phosphorylation a critical process for energy production in cells. Through this pathway COX6B1 interacts with other proteins such as cytochrome c and other subunits of cytochrome c oxidase like COX2 and COX4 which are part of the same complex. These interactions ensure the efficiency of ATP generation from ADP.

Associated diseases and disorders

Mutations or dysfunctions in the COX6B1 gene can lead to mitochondrial conditions such as Complex IV deficiency and Leigh syndrome. These conditions often result in energy production deficits and neurological impairment. COX6B1 is linked to other proteins like COX4 which when mutated can also contribute to similar mitochondrial disorders. Identifying changes in COX6B1 can provide insights into mitochondrial pathologies and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (ab248401), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (ab248401)

    This data was developed using Anti-COX6B1 antibody [EPR7647] ab131277, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling COX6B1 with Anti-COX6B1 antibody [EPR7647] ab131277 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (ab248401), expandable thumbnail

    Western blot - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (ab248401)

    This data was developed using Anti-COX6B1 antibody [EPR7647] ab131277, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-COX6B1 antibody [EPR7647] (Anti-COX6B1 antibody [EPR7647] ab131277) at 1/1000 dilution

    Lane 1: HL60 cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: Caco-2 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 10 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (ab248401), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (ab248401)

    This data was developed using Anti-COX6B1 antibody [EPR7647] ab131277, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labelling COX6B1 with Anti-COX6B1 antibody [EPR7647] ab131277 at 1/50 dilution.

  • OI-RD Scanning - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (ab248401), expandable thumbnail

    OI-RD Scanning - Anti-COX6B1 antibody [EPR7647] - BSA and Azide free (ab248401)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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