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Rabbit Recombinant Monoclonal COX7B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-COX7B antibody [EPR9326(B)] (AB140629), expandable thumbnail
  • Western blot - Anti-COX7B antibody [EPR9326(B)] (AB140629), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (AB140629), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (AB140629), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9326(B)] (AB140629), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Tested
Mouse
Predicted
Not recommended
Not recommended
Predicted
Predicted
Rat
Predicted
Not recommended
Not recommended
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix (By similarity). Plays a role in proper central nervous system (CNS) development in vertebrates (PubMed:23122588).

Alternative names

Cytochrome c oxidase polypeptide VIIb, COX7B

Recommended products

Rabbit Recombinant Monoclonal COX7B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9326(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

COX7B also known as Cytochrome c oxidase subunit 7B is a small protein with a molecular mass of approximately 11 kDa. It performs a mechanical function as a component of the cytochrome c oxidase (COX) complex which is the terminal enzyme of the mitochondrial electron transport chain. COX7B is widely expressed in tissues with high energy demand such as the heart muscle and brain. The protein enables the electron transport chain to conduct its role efficiently by aiding the enzyme's proper assembly and stability.

Biological function summary

Within the architecture of aerobic respiration COX7B functions as a vital component of the protein complex cytochrome c oxidase also referred to as Complex IV. This complex is important for the last step of the electron transport chain facilitating the transfer of electrons from cytochrome c to molecular oxygen. During this process COX7B participates in promoting the creation of a proton gradient across the mitochondrial inner membrane which is an important step in ATP synthesis.

Pathways

Multiple cellular energy production pathways include COX7B's activity such as oxidative phosphorylation and the mitochondrial respiratory chain pathway. It interacts with proteins like cytochrome c and other subunits within Complex IV to ensure effective cellular respiration. By acting in the electron transport chain COX7B helps in controlling the flow of electrons and maintaining mitochondrial function which is essential for metabolic balance.

Associated diseases and disorders

Mitochondrial dysfunctions involving COX7B have associations with particular conditions like Leigh syndrome and mitochondrial complex IV deficiency. These disorders connect COX7B to the pathology where energy production becomes compromised. Other proteins such as those in the COX complex and components of the mitochondrial maze link indirectly with COX7B contributing to disease understanding due to their shared involvement in respiratory defects.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629), expandable thumbnail

    Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629)

    Lanes 1-3: Merged signal (red and green). Green - ab140629 observed at 13 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab140629 Anti-COX7B antibody [EPR9326(B)] was shown to specifically react with COX7B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265549 (knockout cell lysate ab257897) was used. Wild-type and COX7B knockout samples were subjected to SDS-PAGE. ab140629 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: COX7B knockout HeLa cell lysate at 20 µg

    Lane 3: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 9 kDa

    Observed band size: 13 kDa

  • Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629), expandable thumbnail

    Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629)

    All lanes: Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: Fetal kidney tissue lysate at 10 µg

    Lane 3: MCF7 cell lysate at 10 µg

    Lane 4: Fetal heart tissue lysate at 10 µg

    Predicted band size: 9 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629)

    Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labelling COX7B with ab140629 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling COX7B with ab140629 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9326(B)] (ab140629), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9326(B)] (ab140629)

    Immunofluorescent staining of 293 cells labelling COX7B with ab140629 at 1/250 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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