AB137094

Anti-COX7B antibody [EPR9327(B)]

RabMAb
Recombinant
KO Validated
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(5 Publications)

Rabbit Recombinant Monoclonal COX7B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Cytochrome c oxidase polypeptide VIIb, COX7B

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

Immunohistochemical analysis using ab137094 showing positive staining in Urinary bladder transitional carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

Immunohistochemical analysis using ab137094 showing positive staining in Lung adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

Immunohistochemical analysis using ab137094 showing positive staining in Thyroid gland papillary carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

Immunohistochemical analysis using ab137094 showing positive staining in Heart tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling COX7B with ab137094 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9327(B)] (AB137094)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling COX7B with ab137094 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9327(B)] (AB137094)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9327(B)] (AB137094)

Immunofluorescent staining of HepG2 cells labelling COX7B with ab137094 at 1/100 dilution.

Western blot - Anti-COX7B antibody [EPR9327(B)] (AB137094)

WB

Unknown

Western blot - Anti-COX7B antibody [EPR9327(B)] (AB137094)

All lanes:

Western blot - Anti-COX7B antibody [EPR9327(B)] (ab137094) at 1/1000 dilution

All lanes:

Fetal heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 9 kDa

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Western blot - Anti-COX7B antibody [EPR9327(B)] (AB137094)

WB

Lab

Western blot - Anti-COX7B antibody [EPR9327(B)] (AB137094)

Lanes 1-3 : Merged signal (red and green). Green - ab137094 observed at 13 kDa. Red - loading control ab8245 observed at 36 kDa.

ab137094 Anti-COX7B antibody [EPR9327(B)] was shown to specifically react with COX7B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265549 (knockout cell lysate ab257897) was used. Wild-type and COX7B knockout samples were subjected to SDS-PAGE. ab137094 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-COX7B antibody [EPR9327(B)] (ab137094) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

COX7B knockout HeLa cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 9 kDa

Observed band size: 13 kDa

false

Carrier free

Anti-COX7B antibody [EPR9327(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9327(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COX7B also known as Cytochrome c oxidase subunit 7B is a small protein with a molecular mass of approximately 11 kDa. It performs a mechanical function as a component of the cytochrome c oxidase (COX) complex which is the terminal enzyme of the mitochondrial electron transport chain. COX7B is widely expressed in tissues with high energy demand such as the heart muscle and brain. The protein enables the electron transport chain to conduct its role efficiently by aiding the enzyme's proper assembly and stability.

Biological function summary

Within the architecture of aerobic respiration COX7B functions as a vital component of the protein complex cytochrome c oxidase also referred to as Complex IV. This complex is important for the last step of the electron transport chain facilitating the transfer of electrons from cytochrome c to molecular oxygen. During this process COX7B participates in promoting the creation of a proton gradient across the mitochondrial inner membrane which is an important step in ATP synthesis.

Pathways

Multiple cellular energy production pathways include COX7B's activity such as oxidative phosphorylation and the mitochondrial respiratory chain pathway. It interacts with proteins like cytochrome c and other subunits within Complex IV to ensure effective cellular respiration. By acting in the electron transport chain COX7B helps in controlling the flow of electrons and maintaining mitochondrial function which is essential for metabolic balance.

Mitochondrial dysfunctions involving COX7B have associations with particular conditions like Leigh syndrome and mitochondrial complex IV deficiency. These disorders connect COX7B to the pathology where energy production becomes compromised. Other proteins such as those in the COX complex and components of the mitochondrial maze link indirectly with COX7B contributing to disease understanding due to their shared involvement in respiratory defects.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix (By similarity). Plays a role in proper central nervous system (CNS) development in vertebrates (PubMed : 23122588).

See full target information Cytochrome c oxidase subunit 7B, mitochondrial

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:6324 PubMed36344503

2022

Specialist multidisciplinary input maximises rare disease diagnoses from whole genome sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

William L Macken,Micol Falabella,Caroline McKittrick,Chiara Pizzamiglio,Rebecca Ellmers,Kelly Eggleton,Cathy E Woodward,Yogen Patel,Robyn Labrum,Rahul Phadke,Mary M Reilly,Catherine DeVile,Anna Sarkozy,Emma Footitt,James Davison,Shamima Rahman,Henry Houlden,Enrico Bugiardini,Rosaline Quinlivan,Michael G Hanna,Jana Vandrovcova,Robert D S Pitceathly

Cancers 14: PubMed36139533

2022

Mitochondrial Protein Cox7b Is a Metabolic Sensor Driving Brain-Specific Metastasis of Human Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marine C N M Blackman,Tania Capeloa,Justin D Rondeau,Luca X Zampieri,Zohra Benyahia,Justine A Van de Velde,Maude Fransolet,Evangelos P Daskalopoulos,Carine Michiels,Christophe Beauloye,Pierre Sonveaux

Science advances 7: PubMed34362731

2021

Longitudinal tracking of neuronal mitochondria delineates PINK1/Parkin-dependent mechanisms of mitochondrial recycling and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Li,Zak Doric,Amandine Berthet,Danielle M Jorgens,Mai K Nguyen,Ivy Hsieh,Julia Margulis,Rebecca Fang,Jayanta Debnath,Hiromi Sesaki,Steve Finkbeiner,Eric Huang,Ken Nakamura

Cell transplantation 26:1286-1300 PubMed28933220

2017

Elucidation of Gene Expression Patterns in the Brain after Spinal Cord Injury.

Applications

WB

Species

Mouse

Ahreum Baek,Sung-Rae Cho,Sung Hoon Kim

Scientific reports 6:27315 PubMed27265476

2016

Synthetic long non-coding RNAs [SINEUPs] rescue defective gene expression in vivo.

Applications

WB

Species

Unspecified reactive species

Alessia Indrieri,Claudia Grimaldi,Silvia Zucchelli,Roberta Tammaro,Stefano Gustincich,Brunella Franco

View all publications

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