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AB185449

Anti-COX8A antibody

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(1 Publication)

Rabbit Polyclonal COX8A antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COX8A aa 1-50.

View Alternative Names

COX8, COX8L, COX8A, Cytochrome c oxidase polypeptide VIII-liver/heart, Cytochrome c oxidase subunit 8-2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX8A antibody (AB185449)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX8A antibody (AB185449)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling COX8A with ab185449 at 1/50 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human COX8A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P10176

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COX8A also known as Cytochrome c oxidase subunit 8A is a component of the cytochrome c oxidase complex which plays a vital role in the electron transport chain within mitochondria. This protein has a molecular mass of approximately 8.7 kDa. COX8A is highly expressed in tissues with high energy demands such as muscle and brain where efficient ATP production is needed.
Biological function summary

COX8A is an integral part of the large cytochrome c oxidase complex also known as Complex IV in the mitochondrial inner membrane. This complex is involved in oxidative phosphorylation where it catalyzes the reduction of oxygen to water a final step of the electron transport chain. COX8A contributes to the correct assembly and stability of the cytochrome c oxidase complex ensuring proper mitochondrial function and energy production.

Pathways

COX8A participates in the oxidative phosphorylation pathway and is an important player in the process that produces ATP from ADP and inorganic phosphate. It functions together with other subunits in Complex IV such as COX2 and COX3 to facilitate efficient electron transfer and proton pumping. Through its activity in these processes COX8A directly influences cellular respiration and energy metabolism which are critical for cellular homeostasis.

COX8A has connections to mitochondrial disorders such as Leigh syndrome and cytochrome c oxidase deficiency. These conditions arise from defects in mitochondrial oxidative phosphorylation affecting tissues with high metabolic demands. Alterations in COX8A along with other complex IV components like COX2 can contribute to the pathogenesis of these diseases as they disrupt ATP production and lead to energy deficits in affected cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information COX8A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37762032

2023

Loss of Delays Mammary Tumor Development and Impairs Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlyn E Simpson,Christina A Staikos,Katrina L Watson,Roger A Moorehead
View all publications
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