Rabbit Polyclonal CP110 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCP110 aa 250-450.
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- Life science alliance 6:2022GPER1 links estrogens to centrosome amplification and chromosomal instability in human colon cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiriam Bühler,Jeanine Fahrländer,Alexander Sauter,Markus Becker,Elisa Wistorf,Matthias Steinfath,Ailine StolzPubMed 36384894
- Cells 10:2021The SZT2 Interactome Unravels New Functions of the KICSTOR Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCecilia Cattelani,Dominik Lesiak,Gudrun Liebscher,Isabel I Singer,Taras Stasyk,Moritz H Wallnöfer,Alexander M Heberle,Corrado Corti,Michael W Hess,Kristian Pfaller,Marcel Kwiatkowski,Peter P Pramstaller,Andrew A Hicks,Kathrin Thedieck,Thomas Müller,Lukas A Huber,Mariana Eca Guimaraes de AraujoPubMed 34685691
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CCP110 aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link O43303
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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2 products for Alternative Product
Target data
Function
Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation (PubMed:17681131, PubMed:17719545, PubMed:23486064, PubMed:30375385, PubMed:35301795). Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (By similarity). Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2 (PubMed:16760425).
Alternative names
CEP110, CP110, KIAA0419, CCP110, Centriolar coiled-coil protein of 110 kDa, Centrosomal protein of 110 kDa, Cep110
Recommended products
Rabbit Polyclonal CP110 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCP110 aa 250-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CCP110 aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link O43303
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CP110 also known as centrosomal protein 110 is a critical component involved in centriole duplication spindle pole organization and ciliogenesis. It has a molecular weight of approximately 110 kDa. This protein is expressed in various tissues but shows higher expression in those with active cell division cycles such as embryonic tissues and cancer cell lines. As a centrosomal protein CP110 localizes to the centrosome an important cellular structure that manages microtubule organization and cell cycle progression.
- Biological function summary
CP110 interacts with several other proteins to regulate centrosome function and cell division. It is a part of the centriole duplication complex which is essential for proper ciliogenesis and cell cycle control. CP110 caps the mother centriole and prevents the formation of cilia when the cell is in a dividing state therefore playing a role in the balance between cell division and the formation of cilia which contribute to cellular signaling and sensory functions.
- Pathways
The centriole duplication and ciliogenesis processes involve CP110 as it interacts with pathways such as the cell cycle and the Sonic Hedgehog signaling pathway. CP110 partners with proteins like CEP97 and CEP290 to manage the length of centrioles and assist in the timely progression of the cell cycle. The regulation by CP110 ensures that centrosome duplication happens correctly preventing errors that might lead to cell division abnormalities.
- Associated diseases and disorders
CP110 has connections to ciliopathies and cancer. In ciliopathies where cilia formation is compromised impaired CP110 regulation can lead to disorders like Bardet-Biedl syndrome. In cancer overexpression or misregulation of CP110 may result in aberrant cell division and tumorigenesis. Another protein Cyclin-dependent kinase 2 (CDK2) plays a role in cell cycle regulation and works closely with CP110 indicating that disturbances in this interaction could also contribute to these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-CP110 antibody (ab243696)
Paraformaldehyde-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling CP110 (Green) with ab243696 at 4 μg/mL in ICC/IF analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CP110 antibody (ab243696)
Formalin-fixed, paraffin-embedded human kidney tissue stained for CP110 using ab243696 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CP110 antibody (ab243696)
Formalin-fixed, paraffin-embedded human placenta tissue stained for CP110 using ab243696 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CP110 antibody (ab243696)
Negative control:Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for CP110 using ab243696 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CP110 antibody (ab243696)
Formalin-fixed, paraffin-embedded human testis tissue stained for CP110 using ab243696 at 1/200 dilution in immunohistochemical analysis.
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