AB99338

Anti-CP110 antibody

Name: Anti-CP110 antibody (ab99338) | Abcam
Brand: Abcam Limited
SKU: AB99338
Price: 535 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal CP110 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CCP110 aa 950 to C-terminus.

View Alternative Names

CEP110, CP110, KIAA0419, CCP110, Centriolar coiled-coil protein of 110 kDa, Centrosomal protein of 110 kDa, Cep110

2 Images

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Immunoprecipitation - Anti-CP110 antibody (AB99338)

Detection of Human CP110 by Immunoprecipitation (IP) from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
1. IP with a CP110 antibody which recognizes an upstream epitope
2. IP with ab99338 at 3 μg/mg lysate
3. Control IgG IP
Subsequent WB detection was performed using ab99338 at 1 μg/ml.

All lanes:

Immunoprecipitation - Anti-CP110 antibody (ab99338)

Predicted band size: 113 kDa

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Unknown

Western blot - Anti-CP110 antibody (AB99338)

All lanes:

Western blot - Anti-CP110 antibody (ab99338) at 0.04 µg/mL

Lane 1:

Hela whole cell lysate at 50 µg

Lane 2:

Hela whole cell lysate at 15 µg

Lane 3:

Hela whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 113 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CCP110 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43303

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CP110 also known as centrosomal protein 110 is a critical component involved in centriole duplication spindle pole organization and ciliogenesis. It has a molecular weight of approximately 110 kDa. This protein is expressed in various tissues but shows higher expression in those with active cell division cycles such as embryonic tissues and cancer cell lines. As a centrosomal protein CP110 localizes to the centrosome an important cellular structure that manages microtubule organization and cell cycle progression.

Biological function summary

CP110 interacts with several other proteins to regulate centrosome function and cell division. It is a part of the centriole duplication complex which is essential for proper ciliogenesis and cell cycle control. CP110 caps the mother centriole and prevents the formation of cilia when the cell is in a dividing state therefore playing a role in the balance between cell division and the formation of cilia which contribute to cellular signaling and sensory functions.

Pathways

The centriole duplication and ciliogenesis processes involve CP110 as it interacts with pathways such as the cell cycle and the Sonic Hedgehog signaling pathway. CP110 partners with proteins like CEP97 and CEP290 to manage the length of centrioles and assist in the timely progression of the cell cycle. The regulation by CP110 ensures that centrosome duplication happens correctly preventing errors that might lead to cell division abnormalities.

CP110 has connections to ciliopathies and cancer. In ciliopathies where cilia formation is compromised impaired CP110 regulation can lead to disorders like Bardet-Biedl syndrome. In cancer overexpression or misregulation of CP110 may result in aberrant cell division and tumorigenesis. Another protein Cyclin-dependent kinase 2 (CDK2) plays a role in cell cycle regulation and works closely with CP110 indicating that disturbances in this interaction could also contribute to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation (PubMed : 17681131, PubMed : 17719545, PubMed : 23486064, PubMed : 30375385, PubMed : 35301795). Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (By similarity). Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2 (PubMed : 16760425).

See full target information CCP110

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70919 PubMed40772309

2025

Prostaglandin E2 and Progesterone Receptor Coordinately Regulate Primary Cilia for Proper Decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Jhen Lee,Yung-Chieh Tsai,Ting-Yu Chen,Yu-Ying Chao,Ruei-Ci Lin,Hui-Ling Tsai,Jen-Yu Wen,Tang K Tang,Pei-Yin Tsai,Pao-Lin Kuo,Chia-Yih Wang

Cell death & disease 16:22 PubMed39820281

2025

Melanophilin-induced primary cilia promote pancreatic cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ying Chao,Ruei-Ci Lin,Ping-Jui Su,Chu-An Wang,Ting-Yuan Tu,Ya-Chin Hou,Yi-Tzui Tsai,I-Chen Peng,Shaw-Jenq Tsai,Yan-Shen Shan,Chia-Yih Wang

Bio-protocol 13:e4812 PubMed37727868

2023

Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Uzbas,Adam C O'Neill

Journal of cellular physiology 237:4487-4503 PubMed36251015

2022

ATM- and ATR-induced primary ciliogenesis promotes cisplatin resistance in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ying Chao,Bu-Miin Huang,I-Chen Peng,Pei-Rong Lee,Yi-Shyun Lai,Wen-Tai Chiu,Yi-Syuan Lin,Shih-Chieh Lin,Jung-Hsuan Chang,Pai-Sheng Chen,Shaw-Jenq Tsai,Chia-Yih Wang

Cell death and differentiation : PubMed33462409

2021

Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yu Chen,Bu-Miin Huang,Tang K Tang,Yu-Ying Chao,Xiao-Yi Xiao,Pei-Rong Lee,Li-Yun Yang,Chia-Yih Wang

Journal of cellular physiology 236:2706-2724 PubMed32869310

2020

Septin 7 is a centrosomal protein that ensures S phase entry and microtubule nucleation by maintaining the abundance of p150.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yu Chen,Tzu-Chien Lin,Pao-Lin Kuo,Zi-Rong Chen,Hui-Ling Cheng,Yu-Ying Chao,Jhih-Siang Syu,Fu-I Lu,Chia-Yih Wang

The Journal of allergy and clinical immunology 128:1207-1215.e1 PubMed21982113

2011

Inflammation-mediated upregulation of centrosomal protein 110, a negative modulator of ciliogenesis, in patients with chronic rhinosinusitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yinyan Lai,Bei Chen,Jianbo Shi,James N Palmer,David W Kennedy,Noam A Cohen

View all publications

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Product promise

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Anti-CP110 antibody

Immunoprecipitation - Anti-CP110 antibody (AB99338)

Western blot - Anti-CP110 antibody (AB99338)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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