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AB251696

Anti-CPA3 antibody

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(9 Publications)

Rabbit Polyclonal CPA3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Mast cell carboxypeptidase A aa 200-400.

View Alternative Names

Mast cell carboxypeptidase A, MC-CPA, Carboxypeptidase A3, CPA3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPA3 antibody (AB251696)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPA3 antibody (AB251696)

Paraffin-embedded human cervix, uterine tissue stained for CPA3 using ab251696 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPA3 antibody (AB251696)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPA3 antibody (AB251696)

Paraffin-embedded human small intestine tissue stained for CPA3 using ab251696 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPA3 antibody (AB251696)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPA3 antibody (AB251696)

Paraffin-embedded human skeletal muscle tissue stained for CPA3 using ab251696 at 1/200 dilution in immunohistochemical analysis. No positivity in myocytes as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPA3 antibody (AB251696)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPA3 antibody (AB251696)

Paraffin-embedded human skin tissue stained for CPA3 using ab251696 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Mast cell carboxypeptidase A aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

P15088

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carboxypeptidase A3 (CPA3) also known as mast cell carboxypeptidase A is an enzyme with a molecular mass of approximately 50 kDa. It belongs to the metallo-carboxypeptidase family and acts as a proenzyme. CPA3 is primarily expressed in mast cells which predominantly reside in connective tissues such as skin respiratory tract and gastrointestinal tract. This protein contributes to the degradation of certain peptides by cleaving carboxy-terminal amino acids particularly aromatic and branched-chain amino acids.
Biological function summary

CPA3 plays an important role in the defense mechanisms of the body. It is involved in the process of mast cell degranulation releasing various mediators that participate in immune responses. CPA3 functions in combination with other enzymes including tryptase and chymase creating a complex interplay for regulating inflammation. It influences vascular permeability and recruitment of other immune cells to sites of infection or injury. Additionally CPA3 can modulate activities of other proteins by processing their precursors or inactivating them.

Pathways

CPA3 integrates into immune response pathways. It contributes significantly to the inflammatory response cascade where it acts alongside proteins like tryptase and chymase to manage the activity of inflammatory peptides. Moreover CPA3 participates in the renin-angiotensin system which affects blood pressure regulation and fluid balance interacting indirectly with angiotensin-converting enzyme (ACE) and others in this critical pathway.

CPA3 relates prominently to allergic conditions such as asthma and anaphylaxis. The enzyme's participation in the degradation of peptide substrates influences the intensity and duration of allergic responses. CPA3 activity also correlates with remodeling of airway tissues in chronic respiratory conditions. Its connection with mast cell tryptase highlights its role in the pathology of systemic mastocytosis as increased expression of CPA3 occurs in this disorder affecting clinical manifestations in patients.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Medical sciences (Basel, Switzerland) 13: PubMed40981193

2025

Mast Cell Association with the Microenvironment of a Phosphaturic Mesenchymal Tumour Secreting Fibroblast Growth Factor 23.

Applications

Unspecified application

Species

Unspecified reactive species

Andrey Kostin,Alexei Lyundup,Alexander Alekhnovich,Aleksandra Prikhodko,Olga Patsap,Sofia Gronskaia,Zhanna Belaya,Olga Lesnyak,Galina Melnichenko,Natalia Mokrysheva,Igor Buchwalow,Markus Tiemann,Dmitrii Atiakshin

International journal of molecular sciences 25: PubMed39596322

2024

Mast Cell Carboxypeptidase A3 Is Associated with Pulmonary Fibrosis Secondary to COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Yatsiri G Meneses-Preza,Ricardo Martínez-Martínez,Claudia Meixueiro-Calderón,Ulises Manuel Hernández,Elizabeth Angelica Retana,María Dolores Ponce-Regalado,Armando Gamboa-Domínguez,Juan Carlos León-Contreras,Samira Muñoz-Cruz,Rogelio Hernández-Pando,Sonia M Pérez-Tapia,Alma D Chávez-Blanco,Enrique Becerril-Villanueva,Rommel Chacón-Salinas

International journal of molecular sciences 25: PubMed39273142

2024

The Contribution of Mast Cells to the Regulation of Elastic Fiber Tensometry in the Skin Dermis of Children with Marfan Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitrii Atiakshin,Ekaterina Nikolaeva,Alla Semyachkina,Andrey Kostin,Artem Volodkin,Sergey Morozov,Michael Ignatyuk,Liudmila Mikhaleva,Grigory Demyashkin,Daniel Elieh-Ali-Komi,Igor Buchwalow,Markus Tiemann

Cells 13: PubMed38786055

2024

Cyto- and Histopographic Assessment of CPA3-Positive Testicular Mast Cells in Obstructive and Non-Obstructive Azoospermia.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitrii Atiakshin,Nina Kulchenko,Andrey Kostin,Michael Ignatyuk,Andrey Protasov,Ilya Klabukov,Denis Baranovskii,Mikhail Faniev,Elina Korovyakova,Irina Chekmareva,Igor Buchwalow,Markus Tiemann

International journal of molecular sciences 24: PubMed37047472

2023

Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitri Atiakshin,Olga Patsap,Andrey Kostin,Lyudmila Mikhalyova,Igor Buchwalow,Markus Tiemann

Histochemistry and cell biology 159:353-361 PubMed36598563

2023

Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitri Atiakshin,Andrey Kostin,Igor Buchwalow,Denis Morozow,Vera Samoilova,Markus Tiemann

International journal of molecular sciences 23: PubMed36012196

2022

Protease Profile of Tumor-Associated Mast Cells in Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitri Atiakshin,Andrey Kostin,Igor Buchwalow,Vera Samoilova,Markus Tiemann

Cells 11: PubMed35159379

2022

Carboxypeptidase A3-A Key Component of the Protease Phenotype of Mast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitri Atiakshin,Andrey Kostin,Ivan Trotsenko,Vera Samoilova,Igor Buchwalow,Markus Tiemann

Frontiers in cell and developmental biology 9:718638 PubMed34540835

2021

Identification of ARHGEF38, NETO2, GOLM1, and SAPCD2 Associated With Prostate Cancer Progression by Bioinformatic Analysis and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuolun Sun,Yunhua Mao,Xu Zhang,Shuo Lu,Hua Wang,Chi Zhang,Chutian Xiao,Yinghao Cao,Yunhao Qing,Yu Wang,Ke Li
View all publications

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