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AB281223

Anti-CPE antibody [EPR23306-39] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal CPE antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Carboxypeptidase E, CPE, Carboxypeptidase H, Enkephalin convertase, Prohormone-processing carboxypeptidase, CPH

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Sandwich ELISA - Anti-CPE antibody [EPR23306-39] - BSA and Azide free (Capture) (AB281223)
  • sELISA

Lab

Sandwich ELISA - Anti-CPE antibody [EPR23306-39] - BSA and Azide free (Capture) (AB281223)

Sandwich ELISA of ab253663 with the capture antibody ab281223 dilution at 2 µg/mL and detector antibody ab281073 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23306-39

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab281223 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CPE. The recommended pair for sandwich ELISA is:
Capture: ab281223, Human CPE Capture Antibody (unconjugated)
Detector: ab281073, Human CPE Detector Antibody (unconjugated)
The reference range value is 0.08-10 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carboxypeptidase E (CPE) also known as carboxypeptidase H is an enzyme with a mass of about 53 kDa. It facilitates the removal of C-terminal amino acid residues from proteins and peptides. CPE is primarily expressed in endocrine and neuroendocrine tissues including the brain pituitary and pancreas. Its expression in these areas suggests significant involvement in the processing and regulation of peptide hormones and neurotransmitters.
Biological function summary

CPE influences the maturation of peptide hormones and neurotransmitters converting inactive precursors into active forms. CPE does not function as part of a larger complex but acts independently to cleave the C-terminal residues. Its activity plays a role in the biosynthesis of hormones like insulin and proinsulin regulating energy homeostasis and metabolic processes.

Pathways

The activities of enzymes like CPE are integral to the prohormone and neuropeptide processing pathways. Within these pathways CPE works alongside other processing enzymes such as prohormone convertases to ensure proper hormone maturation and secretion. In these pathways CPE connects with proteins like chromogranin A which assists in the storage and trafficking of secretory granules in neuroendocrine cells.

Dysregulation of CPE has links to obesity and type 2 diabetes due to its role in peptide hormone processing. Altered CPE function can impair the maturation of insulin and other hormones contributing to these metabolic conditions. In addition relationships between CPE and other proteins such as proinsulin and insulin further highlight its significance in the pathology of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sorting receptor that directs prohormones to the regulated secretory pathway. Also acts as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.
See full target information Carboxypeptidase E
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com