AB73287

Anti-CPEB1 antibody

Name: Anti-CPEB1 antibody (ab73287) | Abcam
Brand: Abcam Limited
SKU: AB73287
Price: 525 USD
Availability: InStock

(1 Review)

(15 Publications)

Rabbit Polyclonal CPEB1 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human CPEB1.

View Alternative Names

CPEB, CPEB1, Cytoplasmic polyadenylation element-binding protein 1, CPE-BP1, CPE-binding protein 1, h-CPEB, hCPEB-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CPEB1 antibody (AB73287)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CPEB1 antibody (AB73287)

ICC/IF image of ab73287 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-CPEB1 antibody (AB73287)

Lane 1:

Western blot - Anti-CPEB1 antibody (ab73287) at 1 µg/mL

Lane 2:

Western blot - Anti-CPEB1 antibody (ab73287) at 2 µg/mL

All lanes:

rat brain tissue lysate at 15 µg

Predicted band size: 63 kDa

Observed band size: 63 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CPEB1. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1) functions mechanically as a regulator of mRNA translation. Often referred to simply as CPEB1 this protein has a mass of approximately 62 kDa. CPEB1 is expressed in various tissues including the brain and ovary indicating its role in diverse physiological processes. It binds to the cytoplasmic polyadenylation element typically found at the 3' untranslated region of mRNAs controlling the length of the poly(A) tail and therefore influencing mRNA stability and translation.

Biological function summary

CPEB1 regulates cell cycle progression synaptic plasticity and gametogenesis. This protein sometimes part of larger ribonucleoprotein complexes acts by coordinating the temporal and spatial translation of target mRNAs. In neurons CPEB1 plays a significant role in the regulation of local protein synthesis impacting synaptic function and plasticity. In reproductive biology it influences oocyte maturation by modulating the translation of mRNAs important for meiotic progression.

Pathways

CPEB1 plays critical roles within pathways associated with long-term potentiation and the cell cycle. In the long-term potentiation pathway CPEB1 works alongside proteins such as eIF2α to enhance synaptic strength and memory formation. In cell cycle regulation CPEB1 affects the translational activation of cyclins thereby controlling the progression through the cell cycle phases. These pathways highlight its involvement in maintaining cellular homeostasis and response to external stimuli.

CPEB1 has been implicated in neurological disorders such as Alzheimer's disease and Fragile X syndrome. In Alzheimer's disease dysregulation of CPEB1 can lead to impaired synaptic function exacerbating cognitive decline. Similarly CPEB1's interaction with FMRP links it to Fragile X syndrome where it influences synaptic plasticity and neuronal development. These associations highlight the importance of CPEB1 in brain health and cognitive functionality.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed : 24990967). In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry (PubMed : 26398195).

See full target information CPEB1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 151: PubMed38785133

2024

Multiple intersecting pathways are involved in CPEB1 phosphorylation and regulation of translation during mouse oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chisato Kunitomi,Mayra Romero,Enrico Maria Daldello,Karen Schindler,Marco Conti

Nature communications 14:416 PubMed36697412

2023

CPEB1-dependent disruption of the mRNA translation program in oocytes during maternal aging.

Applications

Unspecified application

Species

Unspecified reactive species

Nozomi Takahashi,Federica Franciosi,Enrico Maria Daldello,Xuan G Luong,Peter Althoff,Xiaotian Wang,Marco Conti

Frontiers in cellular neuroscience 16:869398 PubMed35496917

2022

Cytoplasmic Polyadenylation Element-Binding Protein 1 Post-transcriptionally Regulates Fragile X Mental Retardation 1 Expression Through 3' Untranslated Region in Central Nervous System Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Souichi Oe,Shinichi Hayashi,Susumu Tanaka,Taro Koike,Yukie Hirahara,Ryohei Seki-Omura,Rio Kakizaki,Sumika Sakamoto,Yosuke Nakano,Yasuko Noda,Hisao Yamada,Masaaki Kitada

Nature communications 13:947 PubMed35177647

2022

CPEB1 directs muscle stem cell activation by reprogramming the translational landscape.

Applications

Unspecified application

Species

Unspecified reactive species

Wenshu Zeng,Lu Yue,Kim S W Lam,Wenxin Zhang,Wai-Kin So,Erin H Y Tse,Tom H Cheung

Diabetes 71:394-411 PubMed35029277

2022

Obesity-Induced miR-455 Upregulation Promotes Adaptive Pancreatic β-Cell Proliferation Through the CPEB1/CDKN1B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qianxing Hu,Jinming Mu,Yuhong Liu,Yue Yang,Yue Liu,Yi Pan,Yanfeng Zhang,Ling Li,Dechen Liu,Jianqiu Chen,Fangfang Zhang,Liang Jin

Development (Cambridge, England) 148: PubMed34636397

2021

Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Mansour Aboelenain,Karen Schindler

FEBS open bio 12:82-94 PubMed34480525

2021

Cpeb1 expression is post-transcriptionally regulated by AUF1, CPEB1, and microRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Souichi Oe,Shinichi Hayashi,Susumu Tanaka,Taro Koike,Yukie Hirahara,Rio Kakizaki,Sumika Sakamoto,Yasuko Noda,Hisao Yamada,Masaaki Kitada

Oncotarget 11:4155-4168 PubMed33227047

2020

CPEB1 orchestrates a fine-tuning of miR-145-5p tumor-suppressive activity on TWIST1 translation in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Rajabi,Win-Yan Liu-Bordes,Marina Pinskaya,Foretek Dominika,Gueorgui Kratassiouk,Guillaume Pinna,Simona Nanni,Antonella Farsetti,Christian Gespach,Arturo Londoño-Vallejo,Irina Groisman

Nucleic acids research 48:3257-3276 PubMed31970406

2020

Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan G Luong,Enrico Maria Daldello,Gabriel Rajkovic,Cai-Rong Yang,Marco Conti

Cellular and molecular life sciences : CMLS 77:3657-3670 PubMed31796991

2019

A role of cellular translation regulation associated with toxic Huntingtin protein.

Applications

Unspecified application

Species

Unspecified reactive species

Hiranmay Joag,Vighnesh Ghatpande,Meghal Desai,Maitheli Sarkar,Anshu Raina,Mrunalini Shinde,Ruta Chitale,Ankita Deo,Tania Bose,Amitabha Majumdar

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CPEB1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CPEB1 antibody (AB73287)

Western blot - Anti-CPEB1 antibody (AB73287)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise