Anti-CPEB4 antibody [EPR27416-141] KO Tested (ab315332)

Knockout Tested Rabbit Recombinant Monoclonal CPEB4 antibody. Suitable for WB, ICC/IF, IP, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-CPEB4 antibody [EPR27416-141] (AB315332), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	IP	IHC-Fr	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Expected	Tested	Not recommended
Mouse	Tested	Tested	Expected	Tested	Expected	Not recommended
Rat	Tested	Expected	Expected	Expected	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information CPEB4

Function

Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR (PubMed:24990967). RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed:24990967). Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (By similarity). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation (PubMed:26398195). Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT (PubMed:22138752). Stimulates proliferation of melanocytes (PubMed:27857118). In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (By similarity).

Alternative names

KIAA1673, CPEB4, Cytoplasmic polyadenylation element-binding protein 4, CPE-BP4, CPE-binding protein 4, hCPEB-4

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal CPEB4 antibody. Suitable for WB, ICC/IF, IP, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR27416-141
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CPEB4 also known as Cytoplasmic Polyadenylation Element Binding Protein 4 plays an important role in post-transcriptional regulation specifically in mRNA polyadenylation. This protein with a molecular weight of about 75 kDa is part of the CPEB family. Researchers have discovered its expression in various tissues including the brain and testes where it binds mRNA to control translation during processes like oocyte maturation and synaptic plasticity.

Biological function summary

The protein influences gene expression by modulating mRNA stability and translational efficiency. CPEB4 is not isolated in function; it interacts with other proteins as a part of the cytoplasmic polyadenylation complex. Through these interactions CPEB4 affects cell cycle progression stress responses and neuronal development therefore integrating environmental signals into cellular responses.

Pathways

The function of CPEB4 fits into critical signaling routes including the MAPK and PI3K-AKT pathways. Within these pathways CPEB4 interacts with proteins like MEK and AKT impacting cell survival growth and differentiation. The protein modulates mRNA translation in response to signaling cues serving as a conduit for transmitting intracellular messages that dictate cellular adaptation and response.

Associated diseases and disorders

Researchers have linked CPEB4 to conditions such as cancer and neurodegenerative diseases. In glioblastoma for example the protein's aberrant expression affects tumorigenesis pathways often in conjunction with proteins like P53 to manipulate cell proliferation and survival. In neurodegenerative conditions alterations in CPEB4 can impact proteins involved in synaptic maintenance thereby contributing to cognitive decline and neural dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Western blot: Anti-CPEB4 antibody [EPR27416-141] (ab315332) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab315332 was shown to bind specifically to CPEB4. A band was observed at 80 kDa in wild-type A549 cell lysates with no signal observed at this size in CPEB4 knockout cell line. To generate this image, wild-type and CPEB4 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CPEB4 antibody [EPR27416-141] (ab315332) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: CPEB4 knockout A549 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 80 kDa
Immunohistochemistry (Frozen sections) - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling CPEB4 with ab315332 at 1/100 (5.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-CPEB4 (ab315332, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, red) on mouse hippocampus. Panel B: anti-CPEB4 stained on mouse hippocampus. Panel C: anti-NeuN stained in neurons of mouse hippocampus. Panel D: anti-GFAP stained in astrocytes of mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315332 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunoprecipitation - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
CPEB4 was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) whole cell lysate with ab315332 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315332 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: U266B1 (human multiple myeloma B lymphocyte) whole cell lysate10 ug

Lane 2: ab315332 IP in U266B1 (human multiple myeloma B lymphocyte) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315332 in U266B1 whole cell lysate
All lanes: Immunoprecipitation - Anti-CPEB4 antibody [EPR27416-141] (ab315332) at 1/30 dilution
All lanes: U266B1 (human multiple myeloma B lymphocyte) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 119s
Flow Cytometry (Intracellular) - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A-204 (human muscle rhabdomyosarcoma cell, Left) / U266B1 (human multiple myeloma B lymphocyte, Right) cells labelling CPEB4 with ab315332 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: A-204.
Immunohistochemistry (Frozen sections) - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh frozen) tissue labeling CPEB4 with ab315332 at 1/100 (5.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Low expression: confocal image showing no staining on mouse kidney (PMID: 17024188). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315332 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling CPEB4 with ab315332 at 1/100 (5.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Low expression: confocal image showing no staining on mouse skeletal muscle (PMID: 17024188). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315332 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling CPEB4 with ab315332 at 1/100 (5.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315332 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U266B1 (human multiple myeloma B lymphocyte) cells labelling CPEB4 with ab315332 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in U266B1 cells. Low expression: A-204. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling CPEB4 with ab315332 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in Neuro-2a cells and showing weak staining in F9 cells. Low expression: F9. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Western blot - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Low expression: kidney (PMID:17024188).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-7: 10 seconds, Lane 8-9: 48 seconds.
All lanes: Western blot - Anti-CPEB4 antibody [EPR27416-141] (ab315332) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 50 µg
Lane 2: Mouse cerebellum tissue lysate at 50 µg
Lane 3: Mouse kidney tissue lysate at 50 µg
Lane 4: Rat brain tissue lysate at 50 µg
Lane 6: Rat hippocampus tissue lysate at 50 µg
Lane 7: Rat kidney tissue lysate at 50 µg
Lane 8: Human brain tissue lysate at 50 µg
Lane 9: Human cerebellum tissue lysate at 50 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 90 kDa, 36 kDa
Western blot - Anti-CPEB4 antibody [EPR27416-141] (ab315332)
Low expression:A-204, A-673, F9.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-CPEB4 antibody [EPR27416-141] (ab315332) at 1/1000 dilution
Lane 1: U266B1 (human mtiple myeloma B lymphocyte) whole cell lysate at 50 µg
Lane 2: A-204 (rat muscle rhabdomyosarcoma) whole cell lysate at 50 µg
Lane 3: A-673 (human muscle ewis sarcoma) whole cell lysate at 50 µg
Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg
Lane 5: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 90 kDa, 36 kDa
Exposure time: 8s