AB224162

Anti-CPEB4 antibody - N-terminal

Name: Anti-CPEB4 antibody - N-terminal (ab224162) | Abcam
Brand: Abcam Limited
SKU: AB224162

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(3 Publications)

Rabbit Polyclonal CPEB4 antibody. N-terminal. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CPEB4 aa 1-100.

View Alternative Names

KIAA1673, CPEB4, Cytoplasmic polyadenylation element-binding protein 4, CPE-BP4, CPE-binding protein 4, hCPEB-4

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-CPEB4 antibody - N-terminal (AB224162)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CPEB4 antibody - N-terminal (AB224162)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CPEB4 (green) using ab224162 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CPEB4 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q17RY0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPEB4 also known as Cytoplasmic Polyadenylation Element Binding Protein 4 plays an important role in post-transcriptional regulation specifically in mRNA polyadenylation. This protein with a molecular weight of about 75 kDa is part of the CPEB family. Researchers have discovered its expression in various tissues including the brain and testes where it binds mRNA to control translation during processes like oocyte maturation and synaptic plasticity.

Biological function summary

The protein influences gene expression by modulating mRNA stability and translational efficiency. CPEB4 is not isolated in function; it interacts with other proteins as a part of the cytoplasmic polyadenylation complex. Through these interactions CPEB4 affects cell cycle progression stress responses and neuronal development therefore integrating environmental signals into cellular responses.

Pathways

The function of CPEB4 fits into critical signaling routes including the MAPK and PI3K-AKT pathways. Within these pathways CPEB4 interacts with proteins like MEK and AKT impacting cell survival growth and differentiation. The protein modulates mRNA translation in response to signaling cues serving as a conduit for transmitting intracellular messages that dictate cellular adaptation and response.

Researchers have linked CPEB4 to conditions such as cancer and neurodegenerative diseases. In glioblastoma for example the protein's aberrant expression affects tumorigenesis pathways often in conjunction with proteins like P53 to manipulate cell proliferation and survival. In neurodegenerative conditions alterations in CPEB4 can impact proteins involved in synaptic maintenance thereby contributing to cognitive decline and neural dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR (PubMed : 24990967). RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed : 24990967). Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (By similarity). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation (PubMed : 26398195). Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT (PubMed : 22138752). Stimulates proliferation of melanocytes (PubMed : 27857118). In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (By similarity).

See full target information CPEB4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 637:496-503 PubMed39633052

2024

Mis-splicing of a neuronal microexon promotes CPEB4 aggregation in ASD.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Garcia-Cabau,Anna Bartomeu,Giulio Tesei,Kai Chit Cheung,Julia Pose-Utrilla,Sara Picó,Andreea Balaceanu,Berta Duran-Arqué,Marcos Fernández-Alfara,Judit Martín,Cesare De Pace,Lorena Ruiz-Pérez,Jesús García,Giuseppe Battaglia,José J Lucas,Rubén Hervás,Kresten Lindorff-Larsen,Raúl Méndez,Xavier Salvatella

The EMBO journal 43:5972-6000 PubMed39448884

2024

Cyclophilin D plays a critical role in the survival of senescent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Margherita Protasoni,Vanessa López-Polo,Camille Stephan-Otto Attolini,Julian Brandariz,Nicolas Herranz,Joaquin Mateo,Sergio Ruiz,Oscar Fernandez-Capetillo,Marta Kovatcheva,Manuel Serrano

Cell 186:4386-4403.e29 PubMed37774678

2023

Human microglial state dynamics in Alzheimer's disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Na Sun,Matheus B Victor,Yongjin P Park,Xushen Xiong,Aine Ni Scannail,Noelle Leary,Shaniah Prosper,Soujanya Viswanathan,Xochitl Luna,Carles A Boix,Benjamin T James,Yosuke Tanigawa,Kyriaki Galani,Hansruedi Mathys,Xueqiao Jiang,Ayesha P Ng,David A Bennett,Li-Huei Tsai,Manolis Kellis

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CPEB4 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-CPEB4 antibody - N-terminal (AB224162)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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