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AB131451

Anti-CPI-17 antibody

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(2 Publications)

Rabbit Polyclonal CPI-17 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R14A aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CPI17, PPP1INL, PPP1R14A, Protein phosphatase 1 regulatory subunit 14A, 17 kDa PKC-potentiated inhibitory protein of PP1, Protein kinase C-potentiated inhibitor protein of 17 kDa, CPI-17

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-CPI-17 antibody (AB131451)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CPI-17 antibody (AB131451)

Immunofluorescent analysis of methanol fixed HeLa cells labelling CKI-17 with ab131451 antibody at a dilution of 1/100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human PPP1R14A aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96A00

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab131451 was purified by affinity chromatography using epitope specific peptide.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPI-17 also known as Protein Kinase C-Potentiated Inhibitor Protein-17 is a protein that acts as a myosin phosphatase inhibitor. It has a molecular mass of approximately 17 kDa. Expressed in various tissues including smooth muscle heart and brain CPI-17 plays an essential role in regulating smooth muscle contractility. The protein modulates the phosphorylation state of myosin light chains by inhibiting myosin phosphatase activity impacting muscle contraction dynamics.
Biological function summary

CPI-17 influences smooth muscle function by modulating the phosphorylation status of myosin light chains. It is not a component of larger protein complexes but interacts directly with myosin phosphatase. The precise regulation of myosin light chain phosphorylation by CPI-17 is critical for maintaining smooth muscle contractile responses affecting functions such as vasoconstriction and peristalsis.

Pathways

CPI-17 plays an important role in the RhoA/ROCK signaling pathway which is key to controlling smooth muscle tension and motility. Through this pathway CPI-17 interacts closely with proteins like ROCK (Rho-associated coiled-coil containing protein kinase). Additionally CPI-17 is associated with the PKC (Protein Kinase C) pathway where its phosphorylation status and inhibitory activity are regulated by PKC enzymes emphasizing its role in cellular signal transduction.

CPI-17 is implicated in conditions such as hypertension and asthma where dysregulated smooth muscle contractility is a factor. In hypertension CPI-17's interaction with the PKC pathway suggests a substantial influence on blood vessel tension while in asthma altered CPI-17 activity affects airway smooth muscle contraction. The link between CPI-17 and PKC proteins under these conditions provides insight into potential therapeutic targets for managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
See full target information PPP1R14A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:357-366 PubMed37755144

2023

MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Tong Liu,Hong Cui,Quan Na,Sishi Liu

Molecular medicine reports 17:898-910 PubMed29115500

2017

Roles and mechanisms of TRPC3 and the PLCγ/PKC/CPI-17 signaling pathway in regulating parturition.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Dongming Zheng,Hong Cui,Sishi Liu,Lijuan Zhang,Caixia Liu
View all publications
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