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AB52174

Anti-CPI-17 (phospho T38) antibody

3

(2 Reviews)

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(5 Publications)

Rabbit Polyclonal CPI-17 phospho T38 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R14A phospho T38 aa 1-100.

View Alternative Names

CPI17, PPP1INL, PPP1R14A, Protein phosphatase 1 regulatory subunit 14A, 17 kDa PKC-potentiated inhibitory protein of PP1, Protein kinase C-potentiated inhibitor protein of 17 kDa, CPI-17

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPI-17 (phospho T38) antibody (AB52174)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPI-17 (phospho T38) antibody (AB52174)

ab52174 at 1/50 dilution staining CPI-17 in human breast carcinoma by Immunohistochemistry, paraffin embedded tissue, in the presence (right image) and absence (left image) of the immunising peptide.

Western blot - Anti-CPI-17 (phospho T38) antibody (AB52174)
  • WB

Unknown

Western blot - Anti-CPI-17 (phospho T38) antibody (AB52174)

Western blot analysis of extracts from RAW264.7 cells, using CPI-17 (Phospho-Thr38) (ab52174)Antibody. The lane on the right is treated with the synthesized peptide.

All lanes:

Western blot - Anti-CPI-17 (phospho T38) antibody (ab52174)

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PPP1R14A phospho T38 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q96A00

Specificity

ab52174 detects endogenous levels of CPI17a only when phosphorylated at threonine 38.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab52174 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPI-17 also known as Protein Kinase C-Potentiated Inhibitor Protein-17 is a protein that acts as a myosin phosphatase inhibitor. It has a molecular mass of approximately 17 kDa. Expressed in various tissues including smooth muscle heart and brain CPI-17 plays an essential role in regulating smooth muscle contractility. The protein modulates the phosphorylation state of myosin light chains by inhibiting myosin phosphatase activity impacting muscle contraction dynamics.
Biological function summary

CPI-17 influences smooth muscle function by modulating the phosphorylation status of myosin light chains. It is not a component of larger protein complexes but interacts directly with myosin phosphatase. The precise regulation of myosin light chain phosphorylation by CPI-17 is critical for maintaining smooth muscle contractile responses affecting functions such as vasoconstriction and peristalsis.

Pathways

CPI-17 plays an important role in the RhoA/ROCK signaling pathway which is key to controlling smooth muscle tension and motility. Through this pathway CPI-17 interacts closely with proteins like ROCK (Rho-associated coiled-coil containing protein kinase). Additionally CPI-17 is associated with the PKC (Protein Kinase C) pathway where its phosphorylation status and inhibitory activity are regulated by PKC enzymes emphasizing its role in cellular signal transduction.

CPI-17 is implicated in conditions such as hypertension and asthma where dysregulated smooth muscle contractility is a factor. In hypertension CPI-17's interaction with the PKC pathway suggests a substantial influence on blood vessel tension while in asthma altered CPI-17 activity affects airway smooth muscle contraction. The link between CPI-17 and PKC proteins under these conditions provides insight into potential therapeutic targets for managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
See full target information PPP1R14A phospho T38
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medic 2020:9189457 PubMed32104199

2020

Improving Small Intestinal Motility in Experimental Acute Necrotising Pancreatitis by Modulating the CPI-17/MLCP Pathway Using Chaiqin Chengqi Decoction.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqi Lin,Chenlong Zhang,Xiaoxin Zhang,Na Shi,Yongjian Wen,Chengxia Han,Dan Du,Tingting Liu,Tao Jin,Lihui Deng,Kun Jiang,Xiaonan Yang,Jia Guo,Anthony Philips,Robert Sutton,John A Windsor,Wei Huang,Ping Xue,Qing Xia

Experimental and therapeutic medicine 16:103-112 PubMed29896230

2018

S-adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient receptor protein 3/protein kinase Cβ/C-kinase-activated protein phosphatase-1 inhibitor of 17 kDa signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ge,Tao Han,Xiaoqiu Li,Lili Shan,Jinhuan Zhang,Yan Hong,Yanqiu Xia,Jun Wang,Mingxiao Hou

Molecular medicine reports 17:898-910 PubMed29115500

2017

Roles and mechanisms of TRPC3 and the PLCγ/PKC/CPI-17 signaling pathway in regulating parturition.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Dongming Zheng,Hong Cui,Sishi Liu,Lijuan Zhang,Caixia Liu

Experimental and therapeutic medicine 14:2221-2227 PubMed28962146

2017

Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance.

Applications

Unspecified application

Species

Unspecified reactive species

Yinyu Yin,Yiping Li,Jian Pan,Ruze Tang,Jie Zhu,Zhenfang Qin,Xiaobing Xu,Jian Wang

Toxicological sciences : an official journal of th 138:446-556 PubMed24449418

2014

Dysfunction of vascular smooth muscle and vascular remodeling by simvastatin.

Applications

Unspecified application

Species

Rat

Seojin Kang,Hyang-Hwa Woo,Keunyoung Kim,Kyung-Min Lim,Ji-Yoon Noh,Moo-Yeol Lee,Young Min Bae,Ok-Nam Bae,Jin-Ho Chung
View all publications
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