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AB276059

Anti-CPLX2 antibody [021] - BSA and Azide free

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Rabbit Recombinant Monoclonal CPLX2 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CPLX2 aa 1 to C-terminus.

View Alternative Names

Complexin-2, Complexin II, Synaphin-1, CPX II, CPLX2

2 Images
Flow Cytometry (Intracellular) - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)

Intracellular flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling CPLX2 with ab276059, followed byFITC-conjugated secondary antibody (black line) compared with a negative control (grey line).The cells were treated according to manufacturer's manual. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)
  • ICC

Unknown

Immunocytochemistry - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)

Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilzed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling CPLX2 with ab276059 at 1/60 dilution, 4°C overnight, followed by Goat Anti-rabbit IgG Alexa Fluor 488-conjugated secondary antibody (green). Cells were blocked with 10% serum prior incubation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

021

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC, Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human CPLX2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6PUV4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPLX2 also known as Complexin-2 is a synaptic protein involved in neurotransmitter release mechanism. It has a molecular mass of approximately 15 kDa. CPLX2 is highly expressed in the brain and is particularly abundant in synaptic vesicles of neuronal cells. It interacts with SNARE proteins which play a significant role in the vesicle fusion process. CPLX2 acts as a regulator of neurotransmitter release influencing the balance between excitation and inhibition in synaptic transmission.
Biological function summary

CPLX2 functions by interacting with other synaptic proteins to facilitate neurotransmitter release at synapses. It forms a complex with SNARE proteins which are essential for synaptic vesicle fusion with the presynaptic membrane. This interaction ensures precise timing of neurotransmitter release which is critical for neural communication. CPLX2’s role in synaptic function highlights its importance in maintaining the efficiency of synaptic signaling.

Pathways

The role of CPLX2 is integral in synaptic vesicle cycle and neurotransmission pathways. It closely interacts with SNARE proteins such as Syntaxin and Synaptobrevin in facilitating vesicle docking and fusion. These interactions are essential for the neurotransmitter release process which is part of the larger synaptic signaling pathway. CPLX2 modulates synaptic plasticity and exocytosis linking it to the regulation of synaptic strength and neural network activity.

Alterations in CPLX2 expression or function have been associated with neurological conditions like epilepsy and schizophrenia. Abnormal CPLX2 activity may lead to disrupted synaptic signaling contributing to the pathophysiology of these disorders. Additionally its connection with SNARE proteins such as SNAP-25 serves as a potential point of intersection where synaptic dysfunction can contribute to disease development. Understanding CPLX2’s influence on neurotransmission provides insights into therapeutic targets for synaptic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles. Also involved in mast cell exocytosis (By similarity).
See full target information CPLX2
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Product promise

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