Rabbit Recombinant Monoclonal CPLX2 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CPLX2 aa 1 to C-terminus.
Images
![Immunocytochemistry - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059), expandable thumbnail](https://content.abcam.com/products/images/cplx2-antibody-021-bsa-and-azide-free-ab276059--immunocytochemistry-img72834.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)")
![Flow Cytometry (Intracellular) - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059), expandable thumbnail](https://content.abcam.com/products/images/cplx2-antibody-021-bsa-and-azide-free-ab276059--flow-cytometry-intracellular-img52468.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CPLX2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PUV4
Reactivity data
Select an application| ICC | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/100.00000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25.00000 - 1/100.00000 | Notes - |
Target data
Function
Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles. Also involved in mast cell exocytosis (By similarity).
Alternative names
Complexin-2, Complexin II, Synaphin-1, CPX II, CPLX2
Recommended products
Rabbit Recombinant Monoclonal CPLX2 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CPLX2 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CPLX2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PUV4
- Carrier free
- Yes
- Clone number
- 021
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CPLX2 also known as Complexin-2 is a synaptic protein involved in neurotransmitter release mechanism. It has a molecular mass of approximately 15 kDa. CPLX2 is highly expressed in the brain and is particularly abundant in synaptic vesicles of neuronal cells. It interacts with SNARE proteins which play a significant role in the vesicle fusion process. CPLX2 acts as a regulator of neurotransmitter release influencing the balance between excitation and inhibition in synaptic transmission.
- Biological function summary
CPLX2 functions by interacting with other synaptic proteins to facilitate neurotransmitter release at synapses. It forms a complex with SNARE proteins which are essential for synaptic vesicle fusion with the presynaptic membrane. This interaction ensures precise timing of neurotransmitter release which is critical for neural communication. CPLX2’s role in synaptic function highlights its importance in maintaining the efficiency of synaptic signaling.
- Pathways
The role of CPLX2 is integral in synaptic vesicle cycle and neurotransmission pathways. It closely interacts with SNARE proteins such as Syntaxin and Synaptobrevin in facilitating vesicle docking and fusion. These interactions are essential for the neurotransmitter release process which is part of the larger synaptic signaling pathway. CPLX2 modulates synaptic plasticity and exocytosis linking it to the regulation of synaptic strength and neural network activity.
- Associated diseases and disorders
Alterations in CPLX2 expression or function have been associated with neurological conditions like epilepsy and schizophrenia. Abnormal CPLX2 activity may lead to disrupted synaptic signaling contributing to the pathophysiology of these disorders. Additionally its connection with SNARE proteins such as SNAP-25 serves as a potential point of intersection where synaptic dysfunction can contribute to disease development. Understanding CPLX2’s influence on neurotransmission provides insights into therapeutic targets for synaptic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunocytochemistry - Anti-CPLX2 antibody [021] - BSA and Azide free (ab276059), expandable thumbnail](https://content.abcam.com/products/images/cplx2-antibody-021-bsa-and-azide-free-ab276059--immunocytochemistry-img72834.jpg "Immunocytochemistry - Anti-CPLX2 antibody [021] - BSA and Azide free (ab276059)")
Immunocytochemistry - Anti-CPLX2 antibody [021] - BSA and Azide free (ab276059)
Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilzed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling CPLX2 with ab276059 at 1/60 dilution, 4°C overnight, followed by Goat Anti-rabbit IgG Alexa Fluor 488-conjugated secondary antibody (green). Cells were blocked with 10% serum prior incubation.
![Flow Cytometry (Intracellular) - Anti-CPLX2 antibody [021] - BSA and Azide free (ab276059), expandable thumbnail](https://content.abcam.com/products/images/cplx2-antibody-021-bsa-and-azide-free-ab276059--flow-cytometry-intracellular-img52468.jpg "Flow Cytometry (Intracellular) - Anti-CPLX2 antibody [021] - BSA and Azide free (ab276059)")
Flow Cytometry (Intracellular) - Anti-CPLX2 antibody [021] - BSA and Azide free (ab276059)
Intracellular flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling CPLX2 with ab276059, followed byFITC-conjugated secondary antibody (black line) compared with a negative control (grey line).The cells were treated according to manufacturer's manual. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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