AB232895

Anti-CPLX2 antibody

Name: Anti-CPLX2 antibody (ab232895) | Abcam
Brand: Abcam Limited
SKU: AB232895
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal CPLX2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CPLX2.

View Alternative Names

Complexin-2, Complexin II, Synaphin-1, CPX II, CPLX2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX2 antibody (AB232895)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX2 antibody (AB232895)

Formalin-fixed, paraffin-embedded human brain tissue stained for CPLX2 using ab232895 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-CPLX2 antibody (AB232895)

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Western blot - Anti-CPLX2 antibody (ab232895) at 2 µg/mL

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Rat spinal cord lysate

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-CPLX2 antibody (AB232895)

All lanes:

Western blot - Anti-CPLX2 antibody (ab232895) at 2 µg/mL

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Mouse cerebrum lysate

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-CPLX2 antibody (AB232895)

All lanes:

Western blot - Anti-CPLX2 antibody (ab232895) at 2 µg/mL

All lanes:

Rat cerebrum lysate

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-CPLX2 antibody (AB232895)

All lanes:

Western blot - Anti-CPLX2 antibody (ab232895) at 2 µg/mL

All lanes:

Recombinant human CPLX2 protein

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CPLX2.

Q6PUV4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab232895 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPLX2 also known as Complexin-2 is a synaptic protein involved in neurotransmitter release mechanism. It has a molecular mass of approximately 15 kDa. CPLX2 is highly expressed in the brain and is particularly abundant in synaptic vesicles of neuronal cells. It interacts with SNARE proteins which play a significant role in the vesicle fusion process. CPLX2 acts as a regulator of neurotransmitter release influencing the balance between excitation and inhibition in synaptic transmission.

Biological function summary

CPLX2 functions by interacting with other synaptic proteins to facilitate neurotransmitter release at synapses. It forms a complex with SNARE proteins which are essential for synaptic vesicle fusion with the presynaptic membrane. This interaction ensures precise timing of neurotransmitter release which is critical for neural communication. CPLX2’s role in synaptic function highlights its importance in maintaining the efficiency of synaptic signaling.

Pathways

The role of CPLX2 is integral in synaptic vesicle cycle and neurotransmission pathways. It closely interacts with SNARE proteins such as Syntaxin and Synaptobrevin in facilitating vesicle docking and fusion. These interactions are essential for the neurotransmitter release process which is part of the larger synaptic signaling pathway. CPLX2 modulates synaptic plasticity and exocytosis linking it to the regulation of synaptic strength and neural network activity.

Alterations in CPLX2 expression or function have been associated with neurological conditions like epilepsy and schizophrenia. Abnormal CPLX2 activity may lead to disrupted synaptic signaling contributing to the pathophysiology of these disorders. Additionally its connection with SNARE proteins such as SNAP-25 serves as a potential point of intersection where synaptic dysfunction can contribute to disease development. Understanding CPLX2’s influence on neurotransmission provides insights into therapeutic targets for synaptic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles. Also involved in mast cell exocytosis (By similarity).

See full target information CPLX2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 13:23739-23756 PubMed34689137

2021

Loganin substantially ameliorates molecular deficits, pathologies and cognitive impairment in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lulin Nie,Kaiwu He,Fengzhu Xie,Shifeng Xiao,Shupeng Li,Jia Xu,Kaiqin Zhang,Chen Yang,Li Zhou,Jianjun Liu,Liangyu Zou,Xifei Yang

Biology open 10: PubMed34100896

2021

RNA-Seq reveals changes in human placental metabolism, transport and endocrinology across the first-second trimester transition.

Applications

Unspecified application

Species

Unspecified reactive species

Malwina Prater,Russell S Hamilton,Hong Wa Yung,Andrew M Sharkey,Paul Robson,N Erlyani Abd Hamid,Eric Jauniaux,D Stephen Charnock-Jones,Graham J Burton,Tereza Cindrova-Davies

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CPLX2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX2 antibody (AB232895)

Western blot - Anti-CPLX2 antibody (AB232895)

Western blot - Anti-CPLX2 antibody (AB232895)

Western blot - Anti-CPLX2 antibody (AB232895)

Western blot - Anti-CPLX2 antibody (AB232895)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

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