Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)

Rabbit Recombinant Monoclonal CPLX3 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Transfected cell lysate - Mouse, Mouse, Rat, Human samples.

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Images

Western blot - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (AB308464), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IHC-Fr	IP
Human	Expected	Tested	Expected	Expected
Mouse	Tested	Tested	Tested	Tested
Rat	Tested	Tested	Not recommended	Expected
Transfected cell lysate - Mouse	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Mouse, Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Transfected cell lysate - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Mouse	Dilution info -	Notes -

Target data

See full target information Cplx3

Function

Complexin that regulates SNARE protein complex-mediated synaptic vesicle fusion (PubMed:19386896). Required for the maintenance of synaptic ultrastructure in the adult retina (PubMed:19386896). Positively regulates synaptic transmission through synaptic vesicle availability and exocytosis of neurotransmitters at photoreceptor ribbon synapses in the retina (PubMed:15911881, PubMed:19386896, PubMed:27335398). Suppresses tonic photoreceptor activity and baseline 'noise' by suppression of Ca(2+) vesicle tonic release and the facilitation of evoked synchronous and asynchronous Ca(2+) vesicle release (PubMed:22694764, PubMed:27335398).

Alternative names

Complexin-3, Complexin III, CPX III, Cplx3

Recommended products

Rabbit Recombinant Monoclonal CPLX3 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Transfected cell lysate - Mouse, Mouse, Rat, Human samples.

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

EPR28433-43

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for rat IHC-Fr.

ab308463 shows weak cross reactivity with Complexin-4.

Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CPLX3 also known as Complexin-3 is a small synaptic protein with a molecular weight of approximately 18 kDa. It is expressed abundantly in the brain specifically in sensory-related areas where it acts at the presynaptic terminal. CPLX3 belongs to the complexin family which includes Complexin-1 Complexin-2 and Complexin-4. Its expression patterns suggest a role in the central nervous system (CNS) in neurons associated with sensory processing.

Biological function summary

Complexin-3 participates in synaptic vesicle exocytosis by binding to the SNARE complex. This interaction helps regulate the release of neurotransmitters which is essential for proper synaptic function. CPLX3 plays a part in the synaptic vesicle cycle and ensures the precise timing of neurotransmitter release. It may also modulate synaptic plasticity which influences learning and memory processes in the CNS.

Pathways

Complexin-3 operates within the synaptic vesicle cycle and neurotransmitter release pathways. It collaborates closely with SNARE proteins like Syntaxin and Synaptobrevin both key for membrane fusion and neurotransmitter release. CPLX3 fine-tunes these processes impacting the efficiency and dynamics of synaptic transmission. Its function in these pathways highlights its relevance in regulating synaptic communication and plasticity.

Associated diseases and disorders

Complexin-3 has been implicated in neurological conditions such as schizophrenia and autism spectrum disorders. Altered expression levels of CPLX3 may disrupt synaptic signaling contributing to the pathophysiology of these conditions. Its interaction with SNARE proteins underlies its potential involvement in these disorders. Understanding CPLX3's role in these diseases can inform therapeutic strategies for managing synaptic dysfunctions associated with neurological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Western blot - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
This data was developed using Anti-CPLX3 antibody [EPR28433-43] ab308463, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer concentration: 5% NFDM/TBST.
Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used as a loading control at 1/5000 dilution.
Anti-CPLX3 antibody [EPR28433-43] ab308463 shows weak cross reactivity with Complexin-4.
All lanes: Western blot - Anti-CPLX3 antibody [EPR28433-43] (Anti-CPLX3 antibody [EPR28433-43] ab308463) at 1/1000 dilution
Lane 1: His-tagged mouse Complexin-3 recombinant protein (aa2-155) at 0.01 µg
Lane 2: His-tagged mouse Complexin-4 recombinant protein (aa1-160) at 0.01 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 20 kDa, 22 kDa
Exposure time: 20s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
Immunohistochemical analysis of paraffin-embedded Rat retina tissue labelling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat retina.The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum.The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labelling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse skeletal muscle.The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID: 34193558).The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
This data was produced using Anti-CPLX3 antibody [EPR28433-43] ab308463, the same antibody clone but in a different formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: skeletal muscle (PMID: 15911881).

Low expression: brain (PMID: 34193558).

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.

Exposure times: Lanes 1-3: 15 seconds; Lanes 4-6: 70 seconds.
All lanes: Western blot - Anti-CPLX3 antibody [EPR28433-43] (Anti-CPLX3 antibody [EPR28433-43] ab308463) at 1/1000 dilution
Lane 1: Mouse retina tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
Lane 4: Rat retina tissue lysate at 20 µg
Lane 5: Rat skeletal muscle tissue lysate at 20 µg
Lane 6: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 20 kDa
Western blot - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
This data was produced using Anti-CPLX3 antibody [EPR28433-43] ab308463, the same antibody clone but in a different formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.

Exposure time: 3 seconds.
All lanes: Western blot - Anti-CPLX3 antibody [EPR28433-43] (Anti-CPLX3 antibody [EPR28433-43] ab308463) at 1/1000 dilution
Lane 1: HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: HEK-293 transfected with mouse CPLX3 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 20 kDa
Exposure time: 3s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labelling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat skeletal muscle.The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunoprecipitation - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
This data was developed using Anti-CPLX3 antibody [EPR28433-43] ab308463, the same antibody clone in a different buffer formulation.CPLX3 was immunoprecipitated from 0.35 mg Mouse retina tissue lysate with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

Lane 1: Mouse retina tissue lysate

Lane 2: abAB308463 IP in Mouse retina tissue lysate

Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CPLX3 antibody [EPR28433-43] ab308463 in mouse retina tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds
All lanes: Immunoprecipitation - Anti-CPLX3 antibody [EPR28433-43] (Anti-CPLX3 antibody [EPR28433-43] ab308463) at 1/30 dilution
All lanes: Mouse retina tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 20 kDa
Exposure time: 15s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labelling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human skeletal muscle.The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labelling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse retina (PMID: 22694764).The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
Immunohistochemical analysis of paraffin-embedded Human retina tissue labelling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human retina.The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
This data was developed using Anti-CPLX3 antibody [EPR28433-43] ab308463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh) tissue labeling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/100 (5.19 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression: confocal image showing no staining on mouse skeletal muscle (PMID: 15911881). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-CPLX3 antibody [EPR28433-43] - BSA and Azide free (ab308464)
This data was developed using Anti-CPLX3 antibody [EPR28433-43] ab308463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina (fresh) tissue labeling CPLX3 with Anti-CPLX3 antibody [EPR28433-43] ab308463 at 1/100 (5.19 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse retina. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CPLX3 antibody [EPR28433-43] ab308463 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.