AB124874

Anti-CPN1 antibody [EPR6229]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CPN1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ACBP, CPN1, Carboxypeptidase N catalytic chain, CPN, Anaphylatoxin inactivator, Arginine carboxypeptidase, Carboxypeptidase N polypeptide 1, Carboxypeptidase N small subunit, Kininase-1, Lysine carboxypeptidase, Plasma carboxypeptidase B, Serum carboxypeptidase N, SCPN

1 Images

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Western blot - Anti-CPN1 antibody [EPR6229] (AB124874)

All lanes:

Western blot - Anti-CPN1 antibody [EPR6229] (ab124874) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

HepG2 lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 27 kDa,48 kDa

false

Carrier free

Anti-CPN1 antibody [EPR6229] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6229

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Note: The 48-kDa form is generated by proteolytic cleavage at the C-terminus. This subunitis cleaved into a peptide", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carboxypeptidase N also known as CPN or arginine carboxypeptidase is a metallo-protease enzyme with a mass of approximately 280 kDa. It exists as a tetrameric complex composed of two heavy and two light subunits. CPN1 shows expression in plasma and certain tissues like the liver influencing its functional enzyme activity. CPN1 cleaves basic amino acids like lysine and arginine from the C-terminal of peptides impacting several protein activation and inactivation processes.

Biological function summary

Carboxypeptidase N influences various physiological and pathological processes. It functions as part of a plasma-associated complex modulating peptides and proteins post-translationally. CPN1 activity regulates wound healing peptide hormone regulation and modulation of inflammatory response. The enzyme's activity influences the balance of active peptides thereby impacting downstream signaling and cellular responses.

Pathways

Carboxypeptidase N plays significant roles in the complement and kinin-kallikrein systems. In the complement system it deactivates anaphylatoxins like C3a and C5a modulating immune responses. Its ability to cleave kinins highlights its participation in the kallikrein-kinin pathway where it interacts with proteins like bradykinin influencing processes like blood pressure regulation and pain sensation.

Mutations or dysregulation of carboxypeptidase N have links with hereditary angioedema and neuropathological disorders. Hereditary angioedema stems from CPN1's role in metabolizing bradykinin which if not regulated leads to excessive edema. Connections between CPN1 and disorders like schistosomiasis exist involving its interaction with complement proteins C3a and C5a implicating this enzyme in immune response misregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.

See full target information CPN1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed33973518

2021

Limited inhibition of multiple nodes in a driver network blocks metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Ekrem Yesilkanal,Dongbo Yang,Andrea Valdespino,Payal Tiwari,Alan U Sabino,Long Chi Nguyen,Jiyoung Lee,Xiao-He Xie,Siqi Sun,Christopher Dann,Lydia Robinson-Mailman,Ethan Steinberg,Timothy Stuhlmiller,Casey Frankenberger,Elizabeth Goldsmith,Gary L Johnson,Alexandre F Ramos,Marsha R Rosner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CPN1 antibody [EPR6229]

Western blot - Anti-CPN1 antibody [EPR6229] (AB124874)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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