AB155675

Anti-CPNE1 antibody

Name: Anti-CPNE1 antibody (ab155675) | Abcam
Brand: Abcam Limited
SKU: AB155675

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(13 Publications)

Rabbit Polyclonal CPNE1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CPNE1 aa 250 to C-terminus.

View Alternative Names

CPN1, CPNE1, Copine-1, Chromobindin 17, Copine I

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE1 antibody (AB155675)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE1 antibody (AB155675)

Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labeling CPNE1 with ab155675 at 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CPNE1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99829

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CPNE1 also known as Copine-1 is a calcium-dependent phospholipid-binding protein. It weighs approximately 59 kDa and is expressed in various tissues including the brain heart and skeletal muscle. CPNE1 has eight conserved regions which include two C2 domains and a von Willebrand factor A domain enabling its function in the cell.

Biological function summary

CPNE1 interacts with membrane lipids and proteins playing a significant role in diverse cellular processes. It is part of a larger complex associated with membrane trafficking and cytoskeletal organization. CPNE1 can influence cellular processes like cell division and motility through its interactions with other proteins often regulating intracellular signaling pathways.

Pathways

CPNE1 is involved in calcium-mediated signaling and the phosphoinositide 3-kinase (PI3K) pathway. The protein interacts with Akt a downstream target of the PI3K pathway therefore influencing cell survival and growth. These interactions underline CPNE1's role in essential cellular functions linking it with other proteins like phospholipase C.

CPNE1 has associations with cancer progression and neurodegenerative diseases. It interacts with proteins like p53 which play important roles in cell cycle regulation and apoptosis. In cancer altered CPNE1 activity can disrupt normal cell signaling potentially leading to uncontrolled cell growth. In neurodegenerative diseases its involvement in cell signaling pathways may contribute to neuronal dysfunction or death.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes (PubMed : 14674885). Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner (PubMed : 14674885). Exhibits calcium-dependent phospholipid binding properties (PubMed : 19539605, PubMed : 9430674). Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner (PubMed : 23263657, PubMed : 25450385). May recruit target proteins to the cell membrane in a calcium-dependent manner (PubMed : 12522145). May function in membrane trafficking (PubMed : 9430674). Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit (PubMed : 18212740). Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL (PubMed : 18212740).

See full target information CPNE1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 16:2233-2247 PubMed39006290

2024

Comprehensive analysis of CPNE1 predicts prognosis and drug resistance in gastric adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyao Li,Miaomiao Ping,Jizheng Guo,Jin Wang

Annals of surgical treatment and research 105:385-395 PubMed38076599

2023

Exploring the role of copine 1 in human colorectal cancer: investigating its association with tumorigenesis and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Kwon Lee,Seung-Jun Lee,Young-Sool Hah,Yeong-Ho Ji,Young-Tae Ju,Young-Joon Lee,Chi-Young Jeong,Ju-Yeon Kim,Ji-Ho Park,Jae-Myung Kim,Jin-Kyu Cho,Han-Gil Kim,Seung-Jin Kwag

Frontiers in cell and developmental biology 11:1157269 PubMed37077419

2023

Comprehensive analysis of prognostic value, immune implication and biological function of CPNE1 in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Haiting Zhou,Yi He,Yongbiao Huang,Rui Li,Hao Zhang,Xiaohui Xia,Huihua Xiong

Cell and tissue research 391:545-560 PubMed36525128

2022

CPNE1 regulates myogenesis through the PERK-eIF2α pathway mediated by endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Chen,Ling Pan,Yuexi Zeng,Xiaonan Zhu,Li You

Journal of clinical laboratory analysis 36: PubMed36310511

2022

Overexpression of Copines-1 is associated with clinicopathological parameters and poor outcome in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yang,Yingjing Wang,Rong Ge,Xiupeng Jia,Congshan Ge,Youqing Cen,Deng Pan

Cancer cell international 22:67 PubMed35139863

2022

CPNE1 is a potential prognostic biomarker, associated with immune infiltrates and promotes progression of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinfang Su,Yongbiao Huang,Yali Wang,Rui Li,Wanjun Deng,Hao Zhang,Huihua Xiong

Cell communication and signaling : CCS 20:16 PubMed35101055

2022

CPNE1 promotes non-small cell lung cancer progression by interacting with RACK1 via the MET signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Anqi Wang,Wen Yang,Yue Li,Yang Zhang,Jieqi Zhou,Ruochen Zhang,Weijie Zhang,Jianjie Zhu,Yuanyuan Zeng,Zeyi Liu,Jian-An Huang

OncoTargets and therapy 14:699-710 PubMed33536762

2021

CPNE1 Enhances Colorectal Cancer Cell Growth, Glycolysis, and Drug Resistance Through Regulating the AKT-GLUT1/HK2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuexia Wang,Shengli Pan,Xinhong He,Ying Wang,Haozhe Huang,Junxiang Chen,Yuhao Zhang,Zhijin Zhang,Xianju Qin

Aging cell 19:e13276 PubMed33274830

2020

Prevalent intron retention fine-tunes gene expression and contributes to cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yao,Dong Ding,Xueping Li,Ting Shen,Haihui Fu,Hua Zhong,Gang Wei,Ting Ni

Molecular carcinogenesis 59:533-544 PubMed32181526

2020

CPNE1 predicts poor prognosis and promotes tumorigenesis and radioresistance via the AKT singling pathway in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihong Shao,Xiaolong Ma,Yufeng Zhang,Yuanyuan Sun,Wenjuan Lv,Kuigang He,Rui Xia,Peijun Wang,Xiaolong Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CPNE1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE1 antibody (AB155675)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise