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AB272682

Anti-CPNE1 antibody

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Rabbit Polyclonal CPNE1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CPNE1 aa 200-300.

View Alternative Names

CPN1, CPNE1, Copine-1, Chromobindin 17, Copine I

3 Images
Western blot - Anti-CPNE1 antibody (AB272682)
  • WB

Unknown

Western blot - Anti-CPNE1 antibody (AB272682)

All lanes:

Western blot - Anti-CPNE1 antibody (ab272682) at 0.4 µg/mL

Lane 1:

RT4 cells (Human urinary bladder cancer cell line)

Lane 2:

U-251 MG cells (Human brain glioma cell line)

Lane 3:

Human plasma

Lane 4:

Human liver

Lane 5:

Human tonsil

Predicted band size: 59 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE1 antibody (AB272682)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE1 antibody (AB272682)

Paraffin-embedded formalin-fixed Human tonsil tissue stained for CPNE1 using ab272682 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-CPNE1 antibody (AB272682)
  • WB

Unknown

Western blot - Anti-CPNE1 antibody (AB272682)

Corresponding CPNE1 RNA-seq data are presented for the same cell lines.

Loading control : Anti-PFN1.

All lanes:

Western blot - Anti-CPNE1 antibody (ab272682) at 0.4 µg/mL

Lane 1:

Caco-2 cells (Human colorectal adenocarcinoma cell line)

Lane 2:

U-2 OS cells (Human bone osteosarcoma epithelial cell line)

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CPNE1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q99829

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50.00000 - 1/200.00000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04000-0.40000 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CPNE1 also known as Copine-1 is a calcium-dependent phospholipid-binding protein. It weighs approximately 59 kDa and is expressed in various tissues including the brain heart and skeletal muscle. CPNE1 has eight conserved regions which include two C2 domains and a von Willebrand factor A domain enabling its function in the cell.
Biological function summary

CPNE1 interacts with membrane lipids and proteins playing a significant role in diverse cellular processes. It is part of a larger complex associated with membrane trafficking and cytoskeletal organization. CPNE1 can influence cellular processes like cell division and motility through its interactions with other proteins often regulating intracellular signaling pathways.

Pathways

CPNE1 is involved in calcium-mediated signaling and the phosphoinositide 3-kinase (PI3K) pathway. The protein interacts with Akt a downstream target of the PI3K pathway therefore influencing cell survival and growth. These interactions underline CPNE1's role in essential cellular functions linking it with other proteins like phospholipase C.

CPNE1 has associations with cancer progression and neurodegenerative diseases. It interacts with proteins like p53 which play important roles in cell cycle regulation and apoptosis. In cancer altered CPNE1 activity can disrupt normal cell signaling potentially leading to uncontrolled cell growth. In neurodegenerative diseases its involvement in cell signaling pathways may contribute to neuronal dysfunction or death.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes (PubMed : 14674885). Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner (PubMed : 14674885). Exhibits calcium-dependent phospholipid binding properties (PubMed : 19539605, PubMed : 9430674). Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner (PubMed : 23263657, PubMed : 25450385). May recruit target proteins to the cell membrane in a calcium-dependent manner (PubMed : 12522145). May function in membrane trafficking (PubMed : 9430674). Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit (PubMed : 18212740). Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL (PubMed : 18212740).
See full target information CPNE1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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