Anti-CPNE3 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE3 antibody (AB236606)

Paraffin-embedded human liver tissue stained for CPNE3 using ab236606 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CPNE3 antibody (AB236606)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CPNE3 (green) using ab236606 at 1/66 dilution in ICC/IF. Secondary antibody is Alexa Fluor^® 488-congugated Goat Anti-Rabbit IgG(H+L).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.

• WB

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Western blot - Anti-CPNE3 antibody (AB236606)

All lanes:

Western blot - Anti-CPNE3 antibody (ab236606) at 1/500 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 60 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE3 antibody (AB236606)

Paraffin-embedded human small intestine tissue stained for CPNE3 using ab236606 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.