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AB97919

Anti-CPNE3 antibody

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(1 Publication)

Rabbit Polyclonal CPNE3 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CPNE3 aa 200 to C-terminus.

View Alternative Names

CPN3, KIAA0636, CPNE3, Copine-3, Copine III

3 Images
Western blot - Anti-CPNE3 antibody (AB97919)
  • WB

Unknown

Western blot - Anti-CPNE3 antibody (AB97919)

7.5% SDS PAGE

All lanes:

Western blot - Anti-CPNE3 antibody (ab97919) at 1/500 dilution

Lane 1:

HeLaS3 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 60 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE3 antibody (AB97919)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE3 antibody (AB97919)

Immunohistochemical analysis of paraffin-embedded ovarian carcinoma, using ab97919 at 1/100 dilution.

Western blot - Anti-CPNE3 antibody (AB97919)
  • WB

Supplier Data

Western blot - Anti-CPNE3 antibody (AB97919)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-CPNE3 antibody (ab97919) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CPNE3 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75131

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPNE3 also known as Copine-3 is a calcium-dependent membrane-binding protein. This protein has a molecular mass of approximately 58 kDa and belongs to the copine family. It consists of two common elements the C2 domain and the A domain known as Von Willebrand factor type A (VWA). CPNE3 expresses in tissues like brain heart and skeletal muscle. These domains enable CPNE3 to associate with cellular membranes in response to calcium signaling implying a role in intracellular signal transduction.
Biological function summary

CPNE3 influences cellular processes such as membrane trafficking signal transduction and cytoskeletal organization. It is not currently known to be a part of any complex but it is thought to mediate interaction between various cellular components through its modular domains. Its role in signal transduction links it to processes like cell movement apoptosis and differentiation. CPNE3 potentially triggers cellular responses by interacting with other proteins and lipids within the cell environment.

Pathways

CPNE3 actively participates in signaling related to calcium influx and integrin pathways. It is associated with the calcium signaling pathway that is significant for various cellular activities like muscle contraction and neurotransmitter release. Additionally CPNE3’s interaction within the integrin signaling pathway implicates its role in extracellular matrix adhesion and cellular assembly. Through these pathways CPNE3 interacts with proteins such as integrins and calmodulin influencing cell adhesion and motility.

CPNE3 relates closely to cancer and cardiovascular diseases. Its increased expression in some cancers suggests a connection to tumor progression and metastasis particularly in ovarian cancer and glioblastoma. CPNE3 is connected to proteins such as EGFR and Akt in the context of cancer which are key players in cell proliferation and survival pathways. In cardiovascular diseases its capability to influence muscle function relates to proteins involved in calcium homeostasis impacting conditions like hypertrophy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-dependent phospholipid-binding protein that plays a role in ERBB2-mediated tumor cell migration in response to growth factor heregulin stimulation (PubMed : 20010870).
See full target information CPNE3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 10:3057-3067 PubMed30214300

2018

Silencing the expression of copine-III enhances the sensitivity of hepatocellular carcinoma cells to the molecular targeted agent sorafenib.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Chen,Zhengkui Jiang,Wenzhou Zhang,Baoxia He
View all publications
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Product promise

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