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AB236613

Anti-CPOX antibody

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(1 Publication)

Rabbit Polyclonal CPOX antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CPOX aa 1-150.

View Alternative Names

CPO, CPX, CPOX, COX, Coprogen oxidase, Coproporphyrinogenase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPOX antibody (AB236613)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPOX antibody (AB236613)

Paraffin-embedded human liver tissue stained for CPOX using ab236613 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-CPOX antibody (AB236613)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CPOX antibody (AB236613)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CPOX (green) using ab236613 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

Western blot - Anti-CPOX antibody (AB236613)
  • WB

Supplier Data

Western blot - Anti-CPOX antibody (AB236613)

All lanes:

Western blot - Anti-CPOX antibody (ab236613) at 1/500 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

A549 (Human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CPOX aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P36551

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPOX or coproporphyrinogen oxidase catalyzes the oxidation of coproporphyrinogen III to protoporphyrinogen IX an important step in heme biosynthesis. CPOX possesses a molecular mass of approximately 37–40 kDa. Expression occurs primarily in the mitochondria of liver and bone marrow cells. This enzyme plays a role in maintaining the balance of porphyrin intermediates and prevents accumulation that may cause cellular toxicity.
Biological function summary

CPOX converts coproporphyrinogen III into protoporphyrinogen IX playing a role in the heme biosynthetic pathway. As part of the terminal steps in heme production this transformation is important for synthesizing heme an essential component of hemoglobin myoglobin and various cytochromes. CPOX is not part of any larger enzyme complex but interacts with adjacent enzymes in the metabolic pathway to ensure efficient heme synthesis.

Pathways

CPOX functions in the heme biosynthesis pathway. This pathway is vital for the production of heme enabling the synthesis of hemoglobin and electron transport chains. CPOX acts sequentially after coproporphyrinogen III oxidase and before protoporphyrinogen IX oxidase. The enzyme works in concert with proteins such as uroporphyrinogen III synthase (UROS) in earlier steps of the pathway affecting overall heme production efficiency.

Mutations or defects in CPOX can lead to hereditary coproporphyria (HCP) a disorder characterized by neurovisceral attacks and photosensitivity. This condition results from the accumulation of porphyrins due to a dysfunctional heme synthesis pathway. Additionally CPOX-related dysfunction can have implications in liver disorders affecting the balance of heme precursors. Improved understanding of CPOX and its interactions with proteins like UROS can provide insight into therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX and participates to the sixth step in the heme biosynthetic pathway.
See full target information CPOX
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 24:534 PubMed38671413

2024

Association of DOK3 and infiltrated tumor-associated macrophages with risk for the prognosis of Porphyromonas gingivalis-infected oral cancer: a 12-year data analysis of 200 patients from a tertiary teaching hospital, Urumqi, China.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Li,Muqiu Li,Wei Wei,Zhengye Wang,Jingwen Yu,Zhongcheng Gong
View all publications
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