AB169766

Anti-CPOX antibody [EPR11927(B)]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal CPOX antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 3 publications.

View Alternative Names

CPO, CPX, CPOX, COX, Coprogen oxidase, Coproporphyrinogenase

3 Images

Unknown

Immunoprecipitation - Anti-CPOX antibody [EPR11927(B)] (AB169766)

Detection of CPOX by Western Blot of Immunoprecipitate.
Human fetal liver lysate (lane 1) or 1X PBS (lane 2) immunoprecipitated using ab169766 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-CPOX antibody [EPR11927(B)] (ab169766) at 1/1000 dilution

Lane 1:

Immunoprecipitation of CPOX from human fetal liver lysate using ab169766 at 1/10 dilution

Lane 2:

1X PBS

Predicted band size: 50 kDa

false

Lab

Western blot - Anti-CPOX antibody [EPR11927(B)] (AB169766)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1/200000 dilution (36KDa).

All lanes:

Western blot - Anti-CPOX antibody [EPR11927(B)] (ab169766) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HepG2 transfected with siRNA specifically targeting CPOX whole cell lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Lane 6:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/100000 dilution

Observed band size: 35 kDa

false

Exposure time: 59s

Unknown

Western blot - Anti-CPOX antibody [EPR11927(B)] (AB169766)

All lanes:

Western blot - Anti-CPOX antibody [EPR11927(B)] (ab169766) at 1/1000 dilution

Lane 1:

K562 lysate at 10 µg

Lane 2:

HCT-116 lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

LnCaP lysate at 10 µg

Lane 5:

Human fetal liver lysate at 10 µg

Lane 6:

HepG2 lysate at 10 µg

Predicted band size: 50 kDa

false

Carrier free

Anti-CPOX antibody [EPR11927(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11927(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPOX or coproporphyrinogen oxidase catalyzes the oxidation of coproporphyrinogen III to protoporphyrinogen IX an important step in heme biosynthesis. CPOX possesses a molecular mass of approximately 37–40 kDa. Expression occurs primarily in the mitochondria of liver and bone marrow cells. This enzyme plays a role in maintaining the balance of porphyrin intermediates and prevents accumulation that may cause cellular toxicity.

Biological function summary

CPOX converts coproporphyrinogen III into protoporphyrinogen IX playing a role in the heme biosynthetic pathway. As part of the terminal steps in heme production this transformation is important for synthesizing heme an essential component of hemoglobin myoglobin and various cytochromes. CPOX is not part of any larger enzyme complex but interacts with adjacent enzymes in the metabolic pathway to ensure efficient heme synthesis.

Pathways

CPOX functions in the heme biosynthesis pathway. This pathway is vital for the production of heme enabling the synthesis of hemoglobin and electron transport chains. CPOX acts sequentially after coproporphyrinogen III oxidase and before protoporphyrinogen IX oxidase. The enzyme works in concert with proteins such as uroporphyrinogen III synthase (UROS) in earlier steps of the pathway affecting overall heme production efficiency.

Mutations or defects in CPOX can lead to hereditary coproporphyria (HCP) a disorder characterized by neurovisceral attacks and photosensitivity. This condition results from the accumulation of porphyrins due to a dysfunctional heme synthesis pathway. Additionally CPOX-related dysfunction can have implications in liver disorders affecting the balance of heme precursors. Improved understanding of CPOX and its interactions with proteins like UROS can provide insight into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX and participates to the sixth step in the heme biosynthetic pathway.

See full target information CPOX

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e2204711 PubMed36307901

2022

Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Yun Peng,Dian-Kui Cai,Ren-Li Zeng,Chao-Yang Zhang,Guan-Cheng Li,Si-Fan Chen,Xiao-Qing Yuan,Li Peng

Cell stem cell 28:524-534.e7 PubMed33202244

2020

CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Gianluca L Russo,Giovanna Sonsalla,Poornemaa Natarajan,Christopher T Breunig,Giorgia Bulli,Juliane Merl-Pham,Sabine Schmitt,Jessica Giehrl-Schwab,Florian Giesert,Martin Jastroch,Hans Zischka,Wolfgang Wurst,Stefan H Stricker,Stefanie M Hauck,Giacomo Masserdotti,Magdalena Götz

Haematologica 104:1756-1767 PubMed30765471

2019

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Ki Soon Kim,Gregory Holmes-Hampton,Anamika Singh,Tracey A Rouault

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CPOX antibody [EPR11927(B)]

Immunoprecipitation - Anti-CPOX antibody [EPR11927(B)] (AB169766)

Western blot - Anti-CPOX antibody [EPR11927(B)] (AB169766)

Western blot - Anti-CPOX antibody [EPR11927(B)] (AB169766)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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