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AB3682

Anti-CPS1 antibody

5

(9 Reviews)

|

(28 Publications)

Rabbit Polyclonal CPS1 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, African green monkey, Vervet monkey samples. Cited in 28 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Cps1.

View Alternative Names

Carbamoyl-phosphate synthetase I, CPSase I, Cps1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (AB3682)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (AB3682)

CPS-1 localisation is consistent with the reticulated pattern of liver mitonchondria.

Rabbit polyclonal to CPS-1 (ab3682), at 1/5000 on COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV5-CPS 1 plasmid (Corvi, M.M and Berthiaume, L.G. unpublished data).

Secondary : donkey anti-rabbit (Texas Red).

Western blot - Anti-CPS1 antibody (AB3682)
  • WB

Unknown

Western blot - Anti-CPS1 antibody (AB3682)

Lane 1 : 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
Lane 2 : cos-7 cells transfected with full length CPS 1. IP with 5 ul of pre-immune serum
Lane 3 : cos-7 cells transfected with full length CPS 1. IP with 2 ul of polyclonal antibody anti CPS 1 (ab3682)
Lane 4 : cos-7 cells transfected with full length CPS 1. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
Lane 5 : 5 ul of 2ml lysate cos-7 cells transfected with empty vector (pCMV5-tr). Before IP
Lane 6 : cos-7 cells transfected with empty vector (pCMV5-tr). IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
Lane 7 : 5 ul of 2ml lysate untransfected cos-7 cells. Before IP
Lane 8 : untransfected cos-7 cells. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682) ab3682 can IP CPS1 transiently expressed in COS-7 cells.

Lane 1 : 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
Lane 2 : cos-7 cells transfected with full length CPS 1. IP

All lanes:

Western blot - Anti-CPS1 antibody (ab3682)

Predicted band size: 165 kDa

false

Western blot - Anti-CPS1 antibody (AB3682)
  • WB

Unknown

Western blot - Anti-CPS1 antibody (AB3682)

Western blot using Rabbit polyclonal to CPS 1 (ab3682). All lanes loaded with 100 ng of purified CPS 1
Lane 1 : ab3682 1/500
Lane 2 : ab3682 1/1000
Lane 3 : ab3682 1/2000 Western blot using Rabbit polyclonal to CPS 1 (ab3682).

All lanes loaded with 100 ng of purified CPS 1

Lane 1 : ab3682 1/500
Lane 2 : ab3682 1/1000
Lane 3 : ab3682 1/2000

All lanes:

Western blot - Anti-CPS1 antibody (ab3682)

Predicted band size: 165 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

African green monkey, Mouse, Rat

Applications

IHC-P, IHC-Fr, ICC/IF, IP, WB

applications

Immunogen

Native Full Length Protein corresponding to Rat Cps1.

P07756

Specificity

When the antibody is used at 1/2000 some lower molecular weight bands appear in COS-7 cells, the extent of which is significantly reduced when the antibody is used at 1/10,000.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "African green monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Vervet monkey": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

OMIM reference: 114010 Alternative name: carbamoylphosphate synthetase 1, CAD
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPS1 also known as carbamoyl-phosphate synthetase 1 is an important mitochondrial enzyme with a mass of around 165 kDa. It acts in the urea cycle by converting ammonia and bicarbonate into carbamoyl phosphate. CPS1 expression occurs in the liver and intestinal enterocytes which situates it in tissues responsible for metabolism and detoxification processes. This enzyme plays a critical role in the removal of excess nitrogen from the body.
Biological function summary

Carbamoyl-phosphate synthetase 1 functions as an initial step enzyme in the urea cycle which is a metabolic pathway for nitrogen disposal. It does not function alone as it forms part of multi-enzyme complexes to enhance efficiency in the cycle. These complexes facilitate the process of converting toxic ammonia into urea which the body can safely excrete. The proper function of CPS1 ensures that ammonia levels remain non-toxic.

Pathways

The urea cycle remains the chief pathway involving CPS1 collaborating with enzymes like ornithine transcarbamylase and argininosuccinate synthetase. This cycle intersects with the mitochondrial respiratory chain showcasing a relationship between CPS1 and cellular energy balance. Besides the urea cycle the enzyme also links to other metabolic functions like gluconeogenesis given its prerequisite for maintaining a stable nitrogen homeostasis.

CPS1 deficiencies can lead to urea cycle disorders like hyperammonemia characterized by elevated ammonia levels in blood. This may result in neurological damage if untreated. Another condition related to CPS-1 is pulmonary arterial hypertension where studies suggest mishandling of ammonia may alter blood pressure regulation. Genetic mutations affecting the CPS1 protein often underlie these conditions highlighting the importance of genetic screening in affected individuals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
See full target information Cps1
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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Journal of xenobiotics 14:907-922 PubMed39051346

2024

Ionic Liquid 1-Octyl-3-Methylimidazolium (M8OI) Is Mono-Oxygenated by CYP3A4 and CYP3A5 in Adult Human Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Alistair C Leitch,Tarek M Abdelghany,Alex Charlton,Martin Cooke,Matthew C Wright

Biomedicines 11: PubMed37893093

2023

A Mouse Model for the Rapid and Binomial Assessment of Putative WNT/β-Catenin Signalling Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Janson Tse,Ryan O'Keefe,Angela Rigopolous,Annalisa L E Carli,Jo Waaler,Stefan Krauss,Matthias Ernst,Michael Buchert

Nature metabolism 5:626-641 PubMed37081161

2023

Amino acids downregulate SIRT4 to detoxify ammonia through the urea cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Hua Hu,Yu-Yang Feng,Yuan-Xin Yang,Hui-Da Ma,Shu-Xian Zhou,Ya-Nan Qiao,Kai-Hui Zhang,Lei Zhang,Lin Huang,Yi-Yuan Yuan,Yan Lin,Xin-Yan Zhang,Yao Li,Hai-Tao Li,Jian-Yuan Zhao,Wei Xu,Shi-Min Zhao

Toxicology in vitro : an international journal published in association with BIBRA 88:105550 PubMed36603777

2023

The methylimidazolium ionic liquid M8OI is a substrate for OCT1 and p-glycoprotein-1 in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Shireen Hedya,Alex Charlton,Alistair C Leitch,Fahad A Aljehani,Benjamin Pinker,Matthew C Wright,Tarek M Abdelghany

Cell reports. Medicine 3:100820 PubMed36384103

2022

In vivo partial reprogramming by bacteria promotes adult liver organ growth without fibrosis and tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Hess,Timothy J Kendall,Maria Pena,Keitaro Yamane,Daniel Soong,Linda Adams,Richard Truman,Anura Rambukkana

Journal of molecular cell biology 14: PubMed35285892

2022

Regulation of the urea cycle by CPS1 O-GlcNAcylation in response to dietary restriction and aging.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wu,Jiayu Liu,Kalina Lapenta,Reina Desrouleaux,Min-Dian Li,Xiaoyong Yang

Biological chemistry 402:1073-1085 PubMed34333885

2021

Characterization of the scavenger cell proteome in mouse and rat liver.

Applications

Unspecified application

Species

Unspecified reactive species

Martha Paluschinski,Cheng Jun Jin,Natalia Qvartskhava,Boris Görg,Marianne Wammers,Judith Lang,Karl Lang,Gereon Poschmann,Kai Stühler,Dieter Häussinger

Nature metabolism 2:1401-1412 PubMed33257855

2020

The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1 mutant lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyeon Kim,Hyun Min Lee,Feng Cai,Bookyung Ko,Chendong Yang,Elizabeth L Lieu,Nefertiti Muhammad,Shawn Rhyne,Kailong Li,Mohamed Haloul,Wen Gu,Brandon Faubert,Akash K Kaushik,Ling Cai,Sahba Kasiri,Ummay Marriam,Kien Nham,Luc Girard,Hui Wang,Xiankai Sun,James Kim,John D Minna,Keziban Unsal-Kacmaz,Ralph J DeBerardinis

PloS one 14:e0218135 PubMed31242206

2019

Expression of serine/threonine protein kinase SGK1F promotes an hepatoblast state in stem cells directed to differentiate into hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Fouzeyyah Alsaeedi,Rachel Wilson,Charlotte Candlish,Ibrahim Ibrahim,Alistair C Leitch,Tarek M Abdelghany,Colin Wilson,Lyle Armstrong,Matthew C Wright

Cell metabolism 29:1274-1290.e9 PubMed30930169

2019

Telomere Dysfunction Induces Sirtuin Repression that Drives Telomere-Dependent Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hisayuki Amano,Arindam Chaudhury,Cristian Rodriguez-Aguayo,Lan Lu,Viktor Akhanov,Andre Catic,Yury V Popov,Eric Verdin,Hannah Johnson,Fabio Stossi,David A Sinclair,Eiko Nakamaru-Ogiso,Gabriel Lopez-Berestein,Jeffrey T Chang,Joel R Neilson,Alan Meeker,Milton Finegold,Joseph A Baur,Ergun Sahin
View all publications
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