Anti-CPS1 antibody [EPR7493-3] (ab129076) is a rabbit monoclonal antibody that is used to detect CPS1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with CPS1 knockout cell line validation
Images
![Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--western-blot-img703.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076)")
![Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--western-blot-img107940.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076)")
![Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--western-blot-img1457.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (AB129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129382.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (AB129076)")
![Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (AB129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--flow-cytometry-intracellular-img414489.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (AB129076)")
Publications
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- Journal of digestive diseases 24:408-4182023Limited role for hyperammonemia in the progression of diet-induced nonalcoholic steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi Xuan Wang,Meng Yu Wang,Rui Xu Yang,Tian Yi Ren,Ze Hua Zhao,Feng Zhi Xin,Jian Gao FanPubMed 37529891
- Molecular therapy. Methods & clinical development 28:220-2372023Characterization of the humanized FRG mouse model and development of an AAV-LK03 variant with improved liver lobular biodistribution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarti Cabanes-Creus,Renina Gale Navarro,Sophia H Y Liao,Suzanne Scott,Rodrigo Carlessi,Ramon Roca-Pinilla,Maddison Knight,Grober Baltazar,Erhua Zhu,Matthew Jones,Elena Denisenko,Alistair R R Forrest,Ian E Alexander,Janina E E Tirnitz-Parker,Leszek LisowskiPubMed 36700121
- Cell metabolism 34:1151-1167.e72022GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRindert Missiaen,Nicole M Anderson,Laura C Kim,Bailey Nance,Michelle Burrows,Nicolas Skuli,Madeleine Carens,Romain Riscal,An Steensels,Fuming Li,M Celeste SimonPubMed 35839757
- Proceedings of the National Academy of Sciences of the United States of America 119:e21186461192022Ferroptosis regulation by the NGLY1/NFE2L1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiovanni C Forcina,Lauren Pope,Magdalena Murray,Wentao Dong,Monther Abu-Remaileh,Carolyn R Bertozzi,Scott J DixonPubMed 35271393
- International journal of molecular sciences 23:2021The Role of TRIP6, ABCC3 and CPS1 Expression in Resistance of Ovarian Cancer to Taxanes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarolina Seborova,Alzbeta Kloudova-Spalenkova,Kamila Koucka,Petr Holy,Marie Ehrlichova,Changwei Wang,Iwao Ojima,Iveta Voleska,Petr Daniel,Kamila Balusikova,Michael Jelinek,Jan Kovar,Lukas Rob,Martin Hruda,Marcela Mrhalova,Pavel Soucek,Radka VaclavikovaPubMed 35008510
- Molecular oncology 16:1091-11182021RASSF1A independence and early galectin-1 upregulation in PIK3CA-induced hepatocarcinogenesis: new therapeutic venues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Scheiter,Katja Evert,Lucas Reibenspies,Antonio Cigliano,Katharina Annweiler,Karolina Müller,Laura-Maria-Giovanna Pöhmerer,Hongwei Xu,Guofei Cui,Timo Itzel,Silvia Materna-Reichelt,Andrea Coluccio,Kamran Honarnejad,Andreas Teufel,Christoph Brochhausen,Frank Dombrowski,Xin Chen,Matthias Evert,Diego F Calvisi,Kirsten UtpatelPubMed 34748271
- Cells 10:2021Spatio-Temporal Multiscale Analysis of Western Diet-Fed Mice Reveals a Translationally Relevant Sequence of Events during NAFLD Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed Ghallab,Maiju Myllys,Adrian Friebel,Julia Duda,Karolina Edlund,Emina Halilbasic,Mihael Vucur,Zaynab Hobloss,Lisa Brackhagen,Brigitte Begher-Tibbe,Reham Hassan,Michael Burke,Erhan Genc,Lynn Johann Frohwein,Ute Hofmann,Christian H Holland,Daniela González,Magdalena Keller,Abdel-Latif Seddek,Tahany Abbas,Elsayed S I Mohammed,Andreas Teufel,Timo Itzel,Sarah Metzler,Rosemarie Marchan,Cristina Cadenas,Carsten Watzl,Michael A Nitsche,Franziska Kappenberg,Tom Luedde,Thomas Longerich,Jörg Rahnenführer,Stefan Hoehme,Michael Trauner,Jan G HengstlerPubMed 34685496
- OncoTargets and therapy 14:1007-10202021Tandem Mass Tag-Based Proteomic Analysis of Potential Biomarkers for Hepatocellular Carcinoma Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Qiang Li,Ge Huang,Bing-Yao Lin,Dongzi Lin,Yan Ma,Zhao Zhang,Tao Chen,Jie ZhouPubMed 33603407
- International journal of molecular sciences 20:2019Differentially Expressed Mitochondrial Proteins in Human MCF7 Breast Cancer Cells Resistant to Paclitaxel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetr Daniel et. al.PubMed 31248089
- eLife 7:2018Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Ren Lin et. al.PubMed 29993362
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
Alternative names
Carbamoyl-phosphate synthetase I, CPSase I, CPS1
Recommended products
Anti-CPS1 antibody [EPR7493-3] (ab129076) is a rabbit monoclonal antibody that is used to detect CPS1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with CPS1 knockout cell line validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR7493-3
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Anti-CPS1 antibody [EPR7493-3] (ab129076) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), in Human, Mouse, Rat samples.
What is the molecular weight of CPS1?
Anti-CPS1 [EPR7493-3] (ab129076) specifically detects a band for CPS1 (UniProt: P31327) at a molecular weight of 165kDa.
Recommended positive controls
WB: Human fetal liver, mouse liver and rat liver tissue lysates and HeLa cell lysate.IHC-P: Human liver tissue.Flow Cyt (intra): HeLa cells.
Trusted by the scientific community
Anti-CPS1 [EPR7493-3] (ab129076) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed
The specificity of Anti-CPS1 antibody [EPR7493-3] (ab129076) has been confirmed by Western blot testing in CPS1 Knockout HeLa cell line, Human CPS1 knockout HeLa cell line ab261809.
Other related products
We have a range of other formats of antibody clone [EPR7493-3] also available for your convenience:
ab129076, HRP - HRP Anti-CPS1 antibody [EPR7493-3] ab198969, Carrier free - Anti-CPS1 antibody [EPR7493-3] - BSA and Azide free ab248283, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-CPS1 antibody [EPR7493-3] ab252114, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-CPS1 antibody [EPR7493-3] ab252115, APC - APC Anti-CPS1 antibody [EPR7493-3] ab319330, PE - PE Anti-CPS1 antibody [EPR7493-3] ab319467, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-CPS1 antibody [EPR7493-3] ab319823, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-CPS1 antibody [EPR7493-3] ab319964, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-CPS1 antibody [EPR7493-3] ab321026
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CPS1 also known as carbamoyl-phosphate synthetase 1 is an important mitochondrial enzyme with a mass of around 165 kDa. It acts in the urea cycle by converting ammonia and bicarbonate into carbamoyl phosphate. CPS1 expression occurs in the liver and intestinal enterocytes which situates it in tissues responsible for metabolism and detoxification processes. This enzyme plays a critical role in the removal of excess nitrogen from the body.
- Biological function summary
Carbamoyl-phosphate synthetase 1 functions as an initial step enzyme in the urea cycle which is a metabolic pathway for nitrogen disposal. It does not function alone as it forms part of multi-enzyme complexes to enhance efficiency in the cycle. These complexes facilitate the process of converting toxic ammonia into urea which the body can safely excrete. The proper function of CPS1 ensures that ammonia levels remain non-toxic.
- Pathways
The urea cycle remains the chief pathway involving CPS1 collaborating with enzymes like ornithine transcarbamylase and argininosuccinate synthetase. This cycle intersects with the mitochondrial respiratory chain showcasing a relationship between CPS1 and cellular energy balance. Besides the urea cycle the enzyme also links to other metabolic functions like gluconeogenesis given its prerequisite for maintaining a stable nitrogen homeostasis.
- Associated diseases and disorders
CPS1 deficiencies can lead to urea cycle disorders like hyperammonemia characterized by elevated ammonia levels in blood. This may result in neurological damage if untreated. Another condition related to CPS-1 is pulmonary arterial hypertension where studies suggest mishandling of ammonia may alter blood pressure regulation. Genetic mutations affecting the CPS1 protein often underlie these conditions highlighting the importance of genetic screening in affected individuals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--western-blot-img703.jpg "Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)")
Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
Lanes 1-3: Merged signal (red and green). Green - ab129076 observed at 165 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab129076 Anti-CPS1 antibody [EPR7493-3] was shown to specifically react with CPS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CPS1 knockout HeLa cell line ab261809 (knockout cell lysate Human CPS1 knockout HeLa cell lysate ab257121) was used. Wild-type and CPS1 knockout samples were subjected to SDS-PAGE. ab129076 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: CPS1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human CPS1 knockout HeLa cell line (Human CPS1 knockout HeLa cell line ab261809)
Lane 3: Human liver tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 165 kDa
Observed band size: 165 kDa
![Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--western-blot-img107940.jpg "Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)")
Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
All lanes: Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution
Lane 1: Fetal liver cell lysate
Lane 2: Mouse liver cell lysate
Lane 3: Rat liver cell lysate
Lane 4: HeLa cell lysate
Predicted band size: 165 kDa
Observed band size: 150 kDa
![Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--western-blot-img1457.jpg "Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)")
Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
Lanes 1-3: Merged signal (red and green). Green - ab129076 observed at 165 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab129076 Anti-CPS1 antibody [EPR7493-3] was shown to specifically react with CPS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CPS1 knockout HeLa cell line ab261809 (knockout cell lysate Human CPS1 knockout HeLa cell lysate ab257121) was used. Wild-type and CPS1 knockout samples were subjected to SDS-PAGE. ab129076 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: CPS1 knockout HeLa cell lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 165 kDa
Observed band size: 165 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (ab129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129382.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (ab129076)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (ab129076)
ab129076, at 1/100 dilution staining CPS1 in formalin fixed paraffin embedded Human liver tissue by immunohistochemistry
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (ab129076), expandable thumbnail](https://content.abcam.com/products/images/cps1-antibody-epr7493-3-ab129076--flow-cytometry-intracellular-img414489.jpg "Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (ab129076)")
Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (ab129076)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CPS1 (red) with ab129076 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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