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AB129076

Anti-CPS1 antibody [EPR7493-3]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

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(14 Publications)

Anti-CPS1 antibody [EPR7493-3] (ab129076) is a rabbit monoclonal antibody detecting CPS1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Carbamoyl-phosphate synthetase I, CPSase I, CPS1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (AB129076)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (AB129076)

ab129076, at 1/100 dilution staining CPS1 in formalin fixed paraffin embedded Human liver tissue by immunohistochemistry

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (AB129076)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (AB129076)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CPS1 (red) with ab129076 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076)
  • WB

Lab

Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076)

Lanes 1-3 : Merged signal (red and green). Green - ab129076 observed at 165 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab129076 Anti-CPS1 antibody [EPR7493-3] was shown to specifically react with CPS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261809 (knockout cell lysate ab257121) was used. Wild-type and CPS1 knockout samples were subjected to SDS-PAGE. ab129076 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CPS1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CPS1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cps1-knockout-hela-cell-line-ab261809'>ab261809</a>)

Lane 3:

Human liver tissue lysate at 20 µg

Predicted band size: 165 kDa

Observed band size: 165 kDa

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Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076)
  • WB

Unknown

Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076)

All lanes:

Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution

Lane 1:

Fetal liver cell lysate

Lane 2:

Mouse liver cell lysate

Lane 3:

Rat liver cell lysate

Lane 4:

HeLa cell lysate

Predicted band size: 165 kDa

Observed band size: 150 kDa

false

Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076)
  • WB

Unknown

Western blot - Anti-CPS1 antibody [EPR7493-3] (AB129076)

Lanes 1-3 : Merged signal (red and green). Green - ab129076 observed at 165 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab129076 Anti-CPS1 antibody [EPR7493-3] was shown to specifically react with CPS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261809 (knockout cell lysate ab257121) was used. Wild-type and CPS1 knockout samples were subjected to SDS-PAGE. ab129076 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CPS1 knockout HeLa cell lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Predicted band size: 165 kDa

Observed band size: 165 kDa

false

  • HRP

    HRP Anti-CPS1 antibody [EPR7493-3]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CPS1 antibody [EPR7493-3]

  • Carrier free

    Anti-CPS1 antibody [EPR7493-3] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CPS1 antibody [EPR7493-3]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CPS1 antibody [EPR7493-3]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CPS1 antibody [EPR7493-3]

  • 660 APC

    APC Anti-CPS1 antibody [EPR7493-3]

  • 578 PE

    PE Anti-CPS1 antibody [EPR7493-3]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CPS1 antibody [EPR7493-3]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7493-3

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-CPS1 antibody [EPR7493-3] (ab129076) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of CPS1?
Anti-CPS1 [EPR7493-3] (ab129076) specifically detects a band for CPS1 (UniProt: P31327) at a molecular weight of 165kDa.

Trusted by the scientific community
Anti-CPS1 [EPR7493-3] (ab129076) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-CPS1 antibody [EPR7493-3] (ab129076) has been confirmed by Western blot testing in CPS1 Knockout HeLa cell line, ab261809.

Other related products
We have a range of other formats of antibody clone [EPR7493-3] also available for your convenience: ab129076, HRP - ab198969, Carrier free - ab248283, Alexa Fluor® 488 - ab252114, Alexa Fluor® 647 - ab252115, APC - ab319330, PE - ab319467, Alexa Fluor® 594 - ab319823, Alexa Fluor® 555 - ab319964, Alexa Fluor® 750 - ab321026

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPS1 also known as carbamoyl-phosphate synthetase 1 is an important mitochondrial enzyme with a mass of around 165 kDa. It acts in the urea cycle by converting ammonia and bicarbonate into carbamoyl phosphate. CPS1 expression occurs in the liver and intestinal enterocytes which situates it in tissues responsible for metabolism and detoxification processes. This enzyme plays a critical role in the removal of excess nitrogen from the body.
Biological function summary

Carbamoyl-phosphate synthetase 1 functions as an initial step enzyme in the urea cycle which is a metabolic pathway for nitrogen disposal. It does not function alone as it forms part of multi-enzyme complexes to enhance efficiency in the cycle. These complexes facilitate the process of converting toxic ammonia into urea which the body can safely excrete. The proper function of CPS1 ensures that ammonia levels remain non-toxic.

Pathways

The urea cycle remains the chief pathway involving CPS1 collaborating with enzymes like ornithine transcarbamylase and argininosuccinate synthetase. This cycle intersects with the mitochondrial respiratory chain showcasing a relationship between CPS1 and cellular energy balance. Besides the urea cycle the enzyme also links to other metabolic functions like gluconeogenesis given its prerequisite for maintaining a stable nitrogen homeostasis.

CPS1 deficiencies can lead to urea cycle disorders like hyperammonemia characterized by elevated ammonia levels in blood. This may result in neurological damage if untreated. Another condition related to CPS-1 is pulmonary arterial hypertension where studies suggest mishandling of ammonia may alter blood pressure regulation. Genetic mutations affecting the CPS1 protein often underlie these conditions highlighting the importance of genetic screening in affected individuals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
See full target information CPS1
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1437738 PubMed39193349

2024

CPS1 augments hepatic glucagon response through CaMKII/FOXO1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Meng Sun,Xin Wu,Meng-Guang Wei,Li-Zeng Zhu,Wen-Hui Wu,Xin-Yue Zhou,Lian-Wen Qi,Qun Liu

American journal of physiology. Gastrointestinal and liver physiology 327:G235-G253 PubMed38915277

2024

SGLT2 inhibition leads to a restoration of hepatic and circulating metabolites involved in the folate cycle and pyrimidine biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ileana Mendez Espinoza,Elijah N D Choos,Carolyn M Ecelbarger,Blythe D Shepard

Journal of digestive diseases 24:408-418 PubMed37529891

2023

Limited role for hyperammonemia in the progression of diet-induced nonalcoholic steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Zi Xuan Wang,Meng Yu Wang,Rui Xu Yang,Tian Yi Ren,Ze Hua Zhao,Feng Zhi Xin,Jian Gao Fan

Molecular therapy. Methods & clinical development 28:220-237 PubMed36700121

2023

Characterization of the humanized FRG mouse model and development of an AAV-LK03 variant with improved liver lobular biodistribution.

Applications

Unspecified application

Species

Unspecified reactive species

Marti Cabanes-Creus,Renina Gale Navarro,Sophia H Y Liao,Suzanne Scott,Rodrigo Carlessi,Ramon Roca-Pinilla,Maddison Knight,Grober Baltazar,Erhua Zhu,Matthew Jones,Elena Denisenko,Alistair R R Forrest,Ian E Alexander,Janina E E Tirnitz-Parker,Leszek Lisowski

Cell metabolism 34:1151-1167.e7 PubMed35839757

2022

GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Rindert Missiaen,Nicole M Anderson,Laura C Kim,Bailey Nance,Michelle Burrows,Nicolas Skuli,Madeleine Carens,Romain Riscal,An Steensels,Fuming Li,M Celeste Simon

Journal of experimental & clinical cancer research : CR 41:192 PubMed35655220

2022

The Hippo pathway effector TAZ induces intrahepatic cholangiocarcinoma in mice and is ubiquitously activated in the human disease.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Cigliano,Shanshan Zhang,Silvia Ribback,Sara Steinmann,Marcella Sini,Cindy E Ament,Kirsten Utpatel,Xinhua Song,Jingxiao Wang,Maria G Pilo,Fabian Berger,Haichuan Wang,Junyan Tao,Xiaolei Li,Giovanni M Pes,Serena Mancarella,Gianluigi Giannelli,Frank Dombrowski,Matthias Evert,Diego F Calvisi,Xin Chen,Katja Evert

Proceedings of the National Academy of Sciences of the United States of America 119:e2118646119 PubMed35271393

2022

Ferroptosis regulation by the NGLY1/NFE2L1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni C Forcina,Lauren Pope,Magdalena Murray,Wentao Dong,Monther Abu-Remaileh,Carolyn R Bertozzi,Scott J Dixon

International journal of molecular sciences 23: PubMed35008510

2021

The Role of TRIP6, ABCC3 and CPS1 Expression in Resistance of Ovarian Cancer to Taxanes.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Seborova,Alzbeta Kloudova-Spalenkova,Kamila Koucka,Petr Holy,Marie Ehrlichova,Changwei Wang,Iwao Ojima,Iveta Voleska,Petr Daniel,Kamila Balusikova,Michael Jelinek,Jan Kovar,Lukas Rob,Martin Hruda,Marcela Mrhalova,Pavel Soucek,Radka Vaclavikova

Molecular oncology 16:1091-1118 PubMed34748271

2021

RASSF1A independence and early galectin-1 upregulation in PIK3CA-induced hepatocarcinogenesis: new therapeutic venues.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Scheiter,Katja Evert,Lucas Reibenspies,Antonio Cigliano,Katharina Annweiler,Karolina Müller,Laura-Maria-Giovanna Pöhmerer,Hongwei Xu,Guofei Cui,Timo Itzel,Silvia Materna-Reichelt,Andrea Coluccio,Kamran Honarnejad,Andreas Teufel,Christoph Brochhausen,Frank Dombrowski,Xin Chen,Matthias Evert,Diego F Calvisi,Kirsten Utpatel

Cells 10: PubMed34685496

2021

Spatio-Temporal Multiscale Analysis of Western Diet-Fed Mice Reveals a Translationally Relevant Sequence of Events during NAFLD Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Ghallab,Maiju Myllys,Adrian Friebel,Julia Duda,Karolina Edlund,Emina Halilbasic,Mihael Vucur,Zaynab Hobloss,Lisa Brackhagen,Brigitte Begher-Tibbe,Reham Hassan,Michael Burke,Erhan Genc,Lynn Johann Frohwein,Ute Hofmann,Christian H Holland,Daniela González,Magdalena Keller,Abdel-Latif Seddek,Tahany Abbas,Elsayed S I Mohammed,Andreas Teufel,Timo Itzel,Sarah Metzler,Rosemarie Marchan,Cristina Cadenas,Carsten Watzl,Michael A Nitsche,Franziska Kappenberg,Tom Luedde,Thomas Longerich,Jörg Rahnenführer,Stefan Hoehme,Michael Trauner,Jan G Hengstler
View all publications
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