AB81552

Anti-CPSF1 antibody

BOND RX™ Validated
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(6 Publications)

Rabbit Polyclonal CPSF1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human CPSF1 aa 1350 to C-terminus.

View Alternative Names

CPSF160, CPSF1, Cleavage and polyadenylation specificity factor subunit 1, Cleavage and polyadenylation specificity factor 160 kDa subunit, CPSF 160 kDa subunit

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF1 antibody (AB81552)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF1 antibody (AB81552)

IHC image of ab81552 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81552, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF1 antibody (AB81552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CPSF1 with ab81552 at 1/200 (1µg/ml). Detection : DAB.

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Immunoprecipitation - Anti-CPSF1 antibody (AB81552)

HeLa cell lysate (1mg) was immunoprecipitated using ab81552 at 3μg/mg lysate (lane 1) or IgG control (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab81552 at 1μg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-CPSF1 antibody (ab81552)

Predicted band size: 161 kDa

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Unknown

Western blot - Anti-CPSF1 antibody (AB81552)

All lanes:

Western blot - Anti-CPSF1 antibody (ab81552) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 161 kDa

Observed band size: 171 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CPSF1 aa 1350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q10570

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPSF1 also known as Cleavage and Polyadenylation Specificity Factor 1 plays an important role in pre-mRNA processing. It has a molecular weight of approximately 160 kDa and is present ubiquitously in various tissues indicating its essential role in cellular function. This protein acts at the 3' end of pre-mRNA facilitating cleavage and polyadenylation essential processes for mRNA maturation and stability. CPSF1 functions as a part of the larger cleavage and polyadenylation specificity factor complex interacting with other proteins to ensure efficient RNA processing.

Biological function summary

CPSF1 is integral to the formation of mature mRNA which is critical for effective gene expression. It contributes to the cleavage and polyadenylation complex that binds to specific RNA sequences to promote proper 3' end formation of mRNA. This complex in which CPSF1 partners with other proteins like CPSF4 and CPSF6 helps stabilize the polyadenylation signal. Without this activity cells struggle with improper mRNA maturation leading to defects in gene expression.

Pathways

CPSF1 plays a significant role in the mRNA processing pathway where it ensures the correct formation of mRNA tails. This function connects it to gene expression regulatory mechanisms such as the splicing pathway. Through its interaction with proteins like CPSF3 CPSF1 maintains the fidelity of mRNA processing which is pivotal for cellular responses and adaptation to various conditions.

CPSF1 has a strong connection to some forms of cancer and neurodegenerative diseases. Mutations or dysregulation in CPSF1 expression have been observed in specific cancer types where they contribute to aberrant gene expression patterns. Additionally disruptions in CPSF1 and its interaction with other proteins like CSTF64 in the neurodegenerative context highlight its broader implications in disease pathogenesis linking altered pre-mRNA processing with disease conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction (PubMed : 14749727). May play a role in eye morphogenesis and the development of retinal ganglion cell projections to the midbrain (By similarity).

See full target information CPSF1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:862 PubMed39604408

2024

YY1 downregulation underlies therapeutic response to molecular targeted agents.

Applications

Unspecified application

Species

Unspecified reactive species

Shichao Zhou,Jingyu Zang,Mei-Chun Cai,Kaiyan Ye,Jin Liu,Pengfei Ma,Jie Wu,Chenyang Dai,Haijiao Lu,Qing Zhang,Junhong Jiang,Tianqing Chu,Ying Shen,Li Tan,Guanglei Zhuang,Xiaojing Zhao,Lan Wang,Yu Zhuang,Yujie Fu

American journal of cancer research 12:4566-4583 PubMed36381317

2022

CPSF1 positively regulates NSDHL by alternative polyadenylation and promotes gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Weibiao Kang,Yang Yang,Changyu Chen,Changjun Yu

Molecular therapy. Nucleic acids 28:219-230 PubMed35402071

2022

mA-induced repression of SIAH1 facilitates alternative splicing of androgen receptor variant 7 by regulating CPSF1.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Xia,Qing Han,Xuehui Duan,Yinjie Zhu,Jiahua Pan,Baijun Dong,Weiliang Xia,Wei Xue,Jianjun Sha

PloS one 15:e0233380 PubMed32437477

2020

Aberrant expression of CPSF1 promotes head and neck squamous cell carcinoma via regulating alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Sakai,Mizuo Ando,Takahito Fukusumi,Shuling Ren,Chao Liu,Jesse Qualliotine,Sunny Haft,Sayed Sadat,Yuki Saito,Theresa W Guo,Guorong Xu,Roman Sasik,Kathleen M Fisch,J Silvio Gutkind,Elana J Fertig,Alfredo A Molinolo,Joseph A Califano

Nature communications 11:1063 PubMed32102997

2020

The role of Mediator and Little Elongation Complex in transcription termination.

Applications

Unspecified application

Species

Unspecified reactive species

Hidehisa Takahashi,Amol Ranjan,Shiyuan Chen,Hidefumi Suzuki,Mio Shibata,Tomonori Hirose,Hiroko Hirose,Kazunori Sasaki,Ryota Abe,Kai Chen,Yanfeng He,Ying Zhang,Ichigaku Takigawa,Tadasuke Tsukiyama,Masashi Watanabe,Satoshi Fujii,Midori Iida,Junichi Yamamoto,Yuki Yamaguchi,Yutaka Suzuki,Masaki Matsumoto,Keiichi I Nakayama,Michael P Washburn,Anita Saraf,Laurence Florens,Shigeo Sato,Chieri Tomomori-Sato,Ronald C Conaway,Joan W Conaway,Shigetsugu Hatakeyama

Nucleic acids research 46:8557-8573 PubMed29860334

2018

SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3' end processing.

Applications

Unspecified application

Species

Unspecified reactive species

Simei Yu,Antonio Jordán-Pla,Antoni Gañez-Zapater,Shruti Jain,Anna Rolicka,Ann-Kristin Östlund Farrants,Neus Visa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CPSF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF1 antibody (AB81552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF1 antibody (AB81552)

Immunoprecipitation - Anti-CPSF1 antibody (AB81552)

Western blot - Anti-CPSF1 antibody (AB81552)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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