AB248172

Anti-CPSF2/CPSF100 antibody [EPR7599] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CPSF2/CPSF100 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

CPSF100, KIAA1367, CPSF2, Cleavage and polyadenylation specificity factor subunit 2, Cleavage and polyadenylation specificity factor 100 kDa subunit, CPSF 100 kDa subunit

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Western blot - Anti-CPSF2/CPSF100 antibody [EPR7599] - BSA and Azide free (AB248172)

This data was developed using ab126760, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CPSF2/CPSF100 antibody [EPR7599] (<a href='/en-us/products/primary-antibodies/cpsf2-cpsf100-antibody-epr7599-ab126760'>ab126760</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7599

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab248172 is the carrier-free version of ab126760.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPSF2 also known as CPSF100 functions as a component of the cleavage and polyadenylation specificity factor complex. This complex plays a critical role in RNA processing by cleaving the 3' end of pre-mRNA and adding a poly(A) tail. CPSF2 has a molecular mass of approximately 77 kilodaltons and is ubiquitously expressed in human tissues with higher expression in cells with active transcription such as liver and testis.

Biological function summary

The function of CPSF2 involves the coordination of mRNA maturation events necessary for proper gene expression. CPSF2 as part of the CPSF complex associates with other key proteins including CPSF73 CPSF1 and cleavage factors like CFIm and CFIIm. These interactions ensure the correct cleavage and polyadenylation of pre-mRNA which is essential for mRNA stability and translation efficiency.

Pathways

CPSF2 contributes significantly to mRNA processing pathways particularly the cleavage and polyadenylation pathway. This pathway is important for the regulation of gene expression and accessory to RNA polymerase II-mediated transcription termination. CPSF2 collaborates with proteins like CPSF73 and Fip1 in these pathways and disruption in their interactions can affect global mRNA processing.

CPSF2's involvement becomes evident in particular cancers and neurodegenerative disorders. Alterations in mRNA processing due to mutations or dysregulation of CPSF2 are linked to some cancer types such as breast cancer. Additionally aberrant mRNA processing observed in neurodegenerative disorders like Alzheimer's disease involves interactions with proteins including Tau and APP hinting at CPSF2's potential role in these pathological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.

See full target information CPSF2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CPSF2/CPSF100 antibody [EPR7599] - BSA and Azide free

Western blot - Anti-CPSF2/CPSF100 antibody [EPR7599] - BSA and Azide free (AB248172)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise