AB99347

Anti-CPSF6 antibody

BOND RX™ Validated
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(11 Publications)

Rabbit Polyclonal CPSF6 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CPSF6 aa 1-50.

View Alternative Names

CFIM68, CPSF6, Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit, Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7, CPSF 68 kDa subunit, CFIm68

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody (AB99347)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody (AB99347)

IHC image of CPSF6 staining in human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99347, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody (AB99347)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CPSF6 with ab99347 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-CPSF6 antibody (AB99347)

Detection of CPSF6 by Western Blot of Immunprecipitate. ab99347 at 1µg/ml staining CPSF6 in HeLa whole cell lysate immunoprecipitated using ab99347 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 1 seconds.

All lanes:

Immunoprecipitation - Anti-CPSF6 antibody (ab99347)

Predicted band size: 59 kDa

false

Unknown

Western blot - Anti-CPSF6 antibody (AB99347)

All lanes:

Western blot - Anti-CPSF6 antibody (ab99347) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 59 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CPSF6 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q16630

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Medaka fish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Tilapia": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab99347 was affinity purified using an epitope specific to CPSF6 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) also referred to as CFIm68 for its molecular weight of approximately 68 kDa plays an important role in mRNA processing. The protein is expressed widely in various tissues emphasizing its broad functional role in cellular mechanisms. CPSF6 operates as an important component of the cleavage and polyadenylation complex where it facilitates the regulation of pre-mRNA 3'-end processing influencing gene expression control.

Biological function summary

CPSF6 influences alternative polyadenylation impacting mRNA maturation by recognizing specific RNA sequences. As part of a broader complex this protein engages in cooperation with components like CPSF5 and CPSF7 showcasing its integrated role in this essential process. The regulation exerted by CPSF6 on polyadenylation modifies the length and stability of mRNA transcripts. This impact on mRNA dynamics is important for normal cellular function and response.

Pathways

CPSF6 integrates into key mRNA processing and maturation pathways affecting transcriptional regulation and mRNA stability. This protein functionally interlinks with RNA polymerase II playing an integral part in the transcriptional machinery. CPSF6 also associates with the spliceosome complex a critical group in mRNA splicing establishing its multi-functional role within gene expression pathways.

CPSF6 associations emerge prominently in cancer and viral infections. Abnormalities in CPSF6 expression or function can contribute to oncogenic processes through dysregulated mRNA processing. Additionally CPSF6 has significant links to HIV where it interacts with viral components affecting virus replication. The relationship between CPSF6 and other proteins like CPSF1 in these contexts highlights its involvement in disease progression mechanisms rendering it a potential target for therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed : 14690600, PubMed : 29276085, PubMed : 8626397, PubMed : 9659921). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed : 14690600, PubMed : 8626397, PubMed : 9659921). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed : 23187700, PubMed : 29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed : 20695905, PubMed : 29276085). CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery (PubMed : 15169763, PubMed : 21295486, PubMed : 29276085). Plays a role in mRNA export (PubMed : 19864460).. (Microbial infection) Binds HIV-1 capsid-nucleocapsid (HIV-1 CA-NC) complexes and might thereby promote the integration of the virus in the nucleus of dividing cells (in vitro).

See full target information CPSF6

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 15:1578163 PubMed40384984

2025

Mycobacterium tuberculosis modulates NUDT21-mediated alternative polyadenylation to enhance FTH1 expression in macrophages and promotes intracellular growth.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqian Liu,Ningjian Cai,Youchao Dai,Xinchun Chen,Xiaobin Zeng

Genome medicine 16:129 PubMed39529134

2024

Identification of pathological pathways centered on circRNA dysregulation in association with irreversible progression of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Yangping Li,Huifeng Shen,Paula Martinez-Feduchi,Xingyu Ji,Peng Teng,Siddharth Krishnakumar,Jian Hu,Li Chen,Yue Feng,Bing Yao

Scientific reports 13:10974 PubMed37414787

2023

Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon HIV-1 entry into the nucleus is important for productive infection.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Luchsinger,KyeongEun Lee,Gonzalo A Mardones,Vineet N KewalRamani,Felipe Diaz-Griffero

Genome biology 22:82 PubMed33706811

2021

SRSF3 and SRSF7 modulate 3'UTR length through suppression or activation of proximal polyadenylation sites and regulation of CFIm levels.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver Daniel Schwich,Nicole Blümel,Mario Keller,Marius Wegener,Samarth Thonta Setty,Melinda Elaine Brunstein,Ina Poser,Igor Ruiz De Los Mozos,Beatrix Suess,Christian Münch,François McNicoll,Kathi Zarnack,Michaela Müller-McNicoll

Journal of virology 93: PubMed31341054

2019

Interferon-Inducible MicroRNA miR-128 Modulates HIV-1 Replication by Targeting TNPO3 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Aurore Bochnakian,Anjie Zhen,Dimitrios G Zisoulis,Adam Idica,Vineet N KewalRamani,Nicholas Neel,Iben Daugaard,Matthias Hamdorf,Scott Kitchen,KyeongEun Lee,Irene Munk Pedersen

Molecular cell 74:118-131.e7 PubMed30819645

2019

The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export.

Applications

Unspecified application

Species

Unspecified reactive species

Suli Chen,Ruijia Wang,Dinghai Zheng,Heng Zhang,Xingya Chang,Ke Wang,Wencheng Li,Jing Fan,Bin Tian,Hong Cheng

Journal of virology 90:7469-7480 PubMed27279606

2016

Complex Interplay between HIV-1 Capsid and MX2-Independent Alpha Interferon-Induced Antiviral Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenzo Bulli,Luis Apolonia,Juliane Kutzner,Darja Pollpeter,Caroline Goujon,Nikolas Herold,Sarah-Marie Schwarz,Yannick Giernat,Oliver T Keppler,Michael H Malim,Torsten Schaller

PLoS pathogens 12:e1005700 PubMed27327622

2016

KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Adarsh Dharan,Sarah Talley,Abhishek Tripathi,João I Mamede,Matthias Majetschak,Thomas J Hope,Edward M Campbell

The Journal of biological chemistry 291:11809-19 PubMed26994143

2016

The Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Subunit of the Capsid-recruited Pre-messenger RNA Cleavage Factor I (CFIm) Complex Mediates HIV-1 Integration into Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Sheeba Rasheedi,Ming-Chieh Shun,Erik Serrao,Gregory A Sowd,Juan Qian,Caili Hao,Twishasri Dasgupta,Alan N Engelman,Jacek Skowronski

Nature 503:402-405 PubMed24196705

2013

HIV-1 evades innate immune recognition through specific cofactor recruitment.

Applications

Species

Human

Jane Rasaiyaah,Choon Ping Tan,Adam J Fletcher,Amanda J Price,Caroline Blondeau,Laura Hilditch,David A Jacques,David L Selwood,Leo C James,Mahdad Noursadeghi,Greg J Towers

View all publications

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Product promise

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Anti-CPSF6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody (AB99347)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody (AB99347)

Immunoprecipitation - Anti-CPSF6 antibody (AB99347)

Western blot - Anti-CPSF6 antibody (AB99347)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise