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AB175237

Anti-CPSF6 antibody [EPR12898]

4

(1 Review)

|

(20 Publications)

Rabbit Recombinant Monoclonal CPSF6 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 20 publications.

View Alternative Names

CFIM68, CPSF6, Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit, Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7, CPSF 68 kDa subunit, CFIm68

6 Images
Flow Cytometry (Intracellular) - Anti-CPSF6 antibody [EPR12898] (AB175237)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CPSF6 antibody [EPR12898] (AB175237)

Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling CPSF6 with ab175237 antibody at 1/10 (red) or a rabbit IgG negative (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] (AB175237)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] (AB175237)

Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling CPSF6 with ab175237 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] (AB175237)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] (AB175237)

Immunohistochemical analysis of paraffin embedded Human breast tissue labeling CPSF6 with ab175237 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CPSF6 antibody [EPR12898] (AB175237)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CPSF6 antibody [EPR12898] (AB175237)

Immunofluorescent analysis of HeLa cells labeling CPSF6 with ab175237 at 1/100.

Immunoprecipitation - Anti-CPSF6 antibody [EPR12898] (AB175237)
  • IP

Unknown

Immunoprecipitation - Anti-CPSF6 antibody [EPR12898] (AB175237)

Immunoprecipitation of CPSF6 from HeLa cell lysate using ab175237 at 1/10 labeled with ab175237 at 1/10.

All lanes:

Immunoprecipitation - Anti-CPSF6 antibody [EPR12898] (ab175237)

Predicted band size: 59 kDa

false

Western blot - Anti-CPSF6 antibody [EPR12898] (AB175237)
  • WB

Supplier Data

Western blot - Anti-CPSF6 antibody [EPR12898] (AB175237)

All lanes:

Western blot - Anti-CPSF6 antibody [EPR12898] (ab175237) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 59 kDa

false

  • Carrier free

    Anti-CPSF6 antibody [EPR12898] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CPSF6 antibody [EPR12898]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CPSF6 antibody [EPR12898]

  • 578 PE

    PE Anti-CPSF6 antibody [EPR12898]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12898

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) also referred to as CFIm68 for its molecular weight of approximately 68 kDa plays an important role in mRNA processing. The protein is expressed widely in various tissues emphasizing its broad functional role in cellular mechanisms. CPSF6 operates as an important component of the cleavage and polyadenylation complex where it facilitates the regulation of pre-mRNA 3'-end processing influencing gene expression control.
Biological function summary

CPSF6 influences alternative polyadenylation impacting mRNA maturation by recognizing specific RNA sequences. As part of a broader complex this protein engages in cooperation with components like CPSF5 and CPSF7 showcasing its integrated role in this essential process. The regulation exerted by CPSF6 on polyadenylation modifies the length and stability of mRNA transcripts. This impact on mRNA dynamics is important for normal cellular function and response.

Pathways

CPSF6 integrates into key mRNA processing and maturation pathways affecting transcriptional regulation and mRNA stability. This protein functionally interlinks with RNA polymerase II playing an integral part in the transcriptional machinery. CPSF6 also associates with the spliceosome complex a critical group in mRNA splicing establishing its multi-functional role within gene expression pathways.

CPSF6 associations emerge prominently in cancer and viral infections. Abnormalities in CPSF6 expression or function can contribute to oncogenic processes through dysregulated mRNA processing. Additionally CPSF6 has significant links to HIV where it interacts with viral components affecting virus replication. The relationship between CPSF6 and other proteins like CPSF1 in these contexts highlights its involvement in disease progression mechanisms rendering it a potential target for therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed : 14690600, PubMed : 29276085, PubMed : 8626397, PubMed : 9659921). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed : 14690600, PubMed : 8626397, PubMed : 9659921). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed : 23187700, PubMed : 29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed : 20695905, PubMed : 29276085). CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery (PubMed : 15169763, PubMed : 21295486, PubMed : 29276085). Plays a role in mRNA export (PubMed : 19864460).. (Microbial infection) Binds HIV-1 capsid-nucleocapsid (HIV-1 CA-NC) complexes and might thereby promote the integration of the virus in the nucleus of dividing cells (in vitro).
See full target information CPSF6
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1012354 PubMed39823525

2025

The nuclear localization signal of CPSF6 governs post-nuclear import steps of HIV-1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rohlfes,Rajalingam Radhakrishnan,Parmit K Singh,Gregory J Bedwell,Alan N Engelman,Adarsh Dharan,Edward M Campbell

Nature communications 16:617 PubMed39805831

2025

Oncogenic role of RARG rearrangements in acute myeloid leukemia resembling acute promyelocytic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Luyao Zhao,Yun Tan,Xufeng Cen,Huan Gao,Huimin Jiang,Ying Liu,Yunxuan Li,Tingting Zhang,Chenxi Zhao,Ting Shi,Guilin Xu,Churan Wang,Jiong Hu,Xia Li,Ya-Zhen Qin,Kankan Wang,Hong-Hu Zhu,Ke Li

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 34:243-255 PubMed39225594

2024

Inhibiting circ_0000673 blocks the progression of colorectal cancer through downregulating CPSF6 via targeting miR-548b-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Li,Tuoyun Yang,Lu Liu,Baorong Hu,Xi Chen,Wenting Zhao,Xin Hai

PLoS pathogens 20:e1012061 PubMed38416782

2024

Downregulation of CPSF6 leads to global mRNA 3' UTR shortening and enhanced antiviral immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Ge,Jingrong Huang,Rong Chen,Yonggui Fu,Tao Ling,Xin Ou,Xiaohui Rong,Youxiang Cheng,Yi Lin,Fengyi Zhou,Chuanjian Lu,Shaochun Yuan,Anlong Xu

Nature communications 13:5879 PubMed36202818

2022

Prion-like low complexity regions enable avid virus-host interactions during HIV-1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Guochao Wei,Naseer Iqbal,Valentine V Courouble,Ashwanth C Francis,Parmit K Singh,Arpa Hudait,Arun S Annamalai,Stephanie Bester,Szu-Wei Huang,Nikoloz Shkriabai,Lorenzo Briganti,Reed Haney,Vineet N KewalRamani,Gregory A Voth,Alan N Engelman,Gregory B Melikyan,Patrick R Griffin,Francisco Asturias,Mamuka Kvaratskhelia

Molecular cell 82:3030-3044.e8 PubMed35764091

2022

An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zhou,Jeremy M Simon,Chengheng Liao,Cheng Zhang,Lianxin Hu,Giada Zurlo,Xijuan Liu,Cheng Fan,Austin Hepperla,Liwei Jia,Vanina Toffessi Tcheuyap,Hua Zhong,Roy Elias,Jin Ye,W Mike Henne,Payal Kapur,Deepak Nijhawan,James Brugarolas,Qing Zhang

Life (Basel, Switzerland) 12: PubMed35207596

2022

Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuya Kusama,Rulan Bai,Yuta Matsuno,Atsushi Ideta,Toshihiro Sakurai,Kentaro Nagaoka,Masatoshi Hori,Kazuhiko Imakawa

Biomedical engineering online 20:67 PubMed34217312

2021

Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Hongbo Zou,Qichao Xie,Lan Zou,Rui Kong,Bijing Mao

Nucleic acids research 49:7330-7346 PubMed34165568

2021

rigrag: high-resolution mapping of genic targeting preferences during HIV-1 integration in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory J Bedwell,Sooin Jang,Wen Li,Parmit K Singh,Alan N Engelman

mBio 12: PubMed33758083

2021

Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Zhong,Jiying Ning,Emerson A Boggs,Sooin Jang,Callen Wallace,Cheryl Telmer,Marcel P Bruchez,Jinwoo Ahn,Alan N Engelman,Peijun Zhang,Simon C Watkins,Zandrea Ambrose
View all publications
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