Rabbit Recombinant Monoclonal CPSF6 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunoprecipitation - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--immunoprecipitation-img148992.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933)")
![Immunocytochemistry/ Immunofluorescence - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--immunocytochemistry-immunofluorescence-img114072.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70071.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136626.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933)")
![Flow Cytometry (Intracellular) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--flow-cytometry-intracellular-img50361.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (AB238933)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
3 products for Alternative Version
Target data
Function
Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:29276085). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:9659921, PubMed:8626397, PubMed:14690600). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:20695905, PubMed:29276085). CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery (PubMed:15169763, PubMed:29276085, PubMed:21295486). Plays a role in mRNA export (PubMed:19864460).(Microbial infection) Binds HIV-1 capsid-nucleocapsid (HIV-1 CA-NC) complexes and might thereby promote the integration of the virus in the nucleus of dividing cells (in vitro).
Alternative names
Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit, Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7, CPSF 68 kDa subunit, CFIm68, CPSF6, CFIM68
Recommended products
Rabbit Recombinant Monoclonal CPSF6 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit, Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7, CPSF 68 kDa subunit, CFIm68, CPSF6, CFIM68
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR12898
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab238933 is the carrier-free version of Anti-CPSF6 antibody [EPR12898] ab175237.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) also referred to as CFIm68 for its molecular weight of approximately 68 kDa plays an important role in mRNA processing. The protein is expressed widely in various tissues emphasizing its broad functional role in cellular mechanisms. CPSF6 operates as an important component of the cleavage and polyadenylation complex where it facilitates the regulation of pre-mRNA 3'-end processing influencing gene expression control.
- Biological function summary
CPSF6 influences alternative polyadenylation impacting mRNA maturation by recognizing specific RNA sequences. As part of a broader complex this protein engages in cooperation with components like CPSF5 and CPSF7 showcasing its integrated role in this essential process. The regulation exerted by CPSF6 on polyadenylation modifies the length and stability of mRNA transcripts. This impact on mRNA dynamics is important for normal cellular function and response.
- Pathways
CPSF6 integrates into key mRNA processing and maturation pathways affecting transcriptional regulation and mRNA stability. This protein functionally interlinks with RNA polymerase II playing an integral part in the transcriptional machinery. CPSF6 also associates with the spliceosome complex a critical group in mRNA splicing establishing its multi-functional role within gene expression pathways.
- Associated diseases and disorders
CPSF6 associations emerge prominently in cancer and viral infections. Abnormalities in CPSF6 expression or function can contribute to oncogenic processes through dysregulated mRNA processing. Additionally CPSF6 has significant links to HIV where it interacts with viral components affecting virus replication. The relationship between CPSF6 and other proteins like CPSF1 in these contexts highlights its involvement in disease progression mechanisms rendering it a potential target for therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--immunoprecipitation-img148992.jpg "Immunoprecipitation - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)")
Immunoprecipitation - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)
Immunoprecipitation of CPSF6 from HeLa cell lysate using Anti-CPSF6 antibody [EPR12898] ab175237 at 1/10 labeled with Anti-CPSF6 antibody [EPR12898] ab175237 at 1/10.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CPSF6 antibody [EPR12898] ab175237).
All lanes: Immunoprecipitation - Anti-CPSF6 antibody [EPR12898] (Anti-CPSF6 antibody [EPR12898] ab175237)
Predicted band size: 59 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--immunocytochemistry-immunofluorescence-img114072.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)")
Immunocytochemistry/ Immunofluorescence - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)
Immunofluorescent analysis of HeLa cells labeling CPSF6 with Anti-CPSF6 antibody [EPR12898] ab175237 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CPSF6 antibody [EPR12898] ab175237).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70071.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)
Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling CPSF6 with Anti-CPSF6 antibody [EPR12898] ab175237 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CPSF6 antibody [EPR12898] ab175237).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136626.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)
Immunohistochemical analysis of paraffin embedded Human breast tissue labeling CPSF6 with Anti-CPSF6 antibody [EPR12898] ab175237 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CPSF6 antibody [EPR12898] ab175237).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933), expandable thumbnail](https://content.abcam.com/products/images/cpsf6-antibody-epr12898-bsa-and-azide-free-ab238933--flow-cytometry-intracellular-img50361.jpg "Flow Cytometry (Intracellular) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)")
Flow Cytometry (Intracellular) - Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)
Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling CPSF6 with Anti-CPSF6 antibody [EPR12898] ab175237 antibody at 1/10 (red) or a rabbit IgG negative (green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CPSF6 antibody [EPR12898] ab175237).
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