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AB272634

Anti-CPSF7 antibody

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(1 Publication)

Rabbit Polyclonal CPSF7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CPSF7 aa 1-150.

View Alternative Names

Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Pre-mRNA cleavage factor Im 59 kDa subunit, CPSF 59 kDa subunit, CFIm59, CPSF7

4 Images
Western blot - Anti-CPSF7 antibody (AB272634)
  • WB

Unknown

Western blot - Anti-CPSF7 antibody (AB272634)

All lanes:

Western blot - Anti-CPSF7 antibody (ab272634) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat bladder carcinoma cell line) cell lysate

Predicted band size: 52 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-CPSF7 antibody (AB272634)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CPSF7 antibody (AB272634)

PFA fixed, Triton X-100 permeabilized human cell line A431 labeling CPSF7 (green) using ab272634 at 2 ug/ml in ICC/IF analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF7 antibody (AB272634)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF7 antibody (AB272634)

Paraffin-embedded formalin-fixed human esophagus tissue stained for CPSF7 using ab272634 at 1/50 in immunohistochemical analysis.

Western blot - Anti-CPSF7 antibody (AB272634)
  • WB

Unknown

Western blot - Anti-CPSF7 antibody (AB272634)

Lane 1:

Western blot - Anti-CPSF7 antibody (ab272634)

Lane 2:

Western blot - Anti-CPSF7 antibody (ab272634) at 0.4 µg/mL

Lane 1:

Molecular weight ladder

Lane 2:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CPSF7 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q8N684

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPSF7 also known as cleavage and polyadenylation specificity factor subunit 7 is a protein that participates in mRNA processing. With a mass of approximately 55 kDa CPSF7 resides in the nucleus of cells where it plays a role in the cleavage and polyadenylation of pre-mRNA. This protein is expressed in various tissues emphasizing its broad role in gene expression regulation across different cell types.
Biological function summary

CPSF7 functions in the formation of the cleavage and polyadenylation complex which is essential for proper mRNA maturation. It interacts with other subunits in this complex to ensure accurate mRNA 3' end processing. This protein participates in determining the polyadenylation site a critical step that influences mRNA stability export and translation.

Pathways

CPSF7 integrates into the post-transcriptional regulation of gene expression. It plays important roles within the mRNA processing pathway and is linked to the regulation of the cell cycle. CPSF7 interacts with other proteins such as CPSF3 within these pathways to mediate these biological processes that are pivotal for efficient gene expression and cell function.

Aberrations in CPSF7 function relate to cancer and autoimmune diseases. In cancer altered expression of CPSF7 may affect tumor growth and progression potentially through interactions with other proteins like CPSF73. Autoimmune diseases could also involve CPSF7 due to disruptions in mRNA processing which might affect immune cell function and regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed : 17024186, PubMed : 29276085, PubMed : 8626397). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed : 17024186, PubMed : 8626397). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed : 23187700, PubMed : 29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed : 20695905, PubMed : 29276085). CPSF7 activates directly the mRNA 3'-processing machinery (PubMed : 29276085). Binds to pA signals in RNA substrates (PubMed : 17024186, PubMed : 8626397).
See full target information CPSF7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:2047-2058 PubMed40235277

2025

The mechanism of dexmedetomidine regulation of the HIF-1α/FUNDC1 axis in myocardial ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenfei Hu,Yidan Huang
View all publications
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Product promise

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