AB72295

Anti-CPSF73 antibody

Name: Anti-CPSF73 antibody (ab72295) | Abcam
Brand: Abcam Limited
SKU: AB72295
Rating: 4 (8 reviews)

(8 Reviews)

(7 Publications)

Rabbit Polyclonal CPSF73 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CPSF3.

View Alternative Names

CPSF73, CPSF3, Cleavage and polyadenylation specificity factor subunit 3, Cleavage and polyadenylation specificity factor 73 kDa subunit, mRNA 3'-end-processing endonuclease CPSF-73, CPSF 73 kDa subunit

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF73 antibody (AB72295)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF73 antibody (AB72295)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling CPSF73 with ab72295 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-CPSF73 antibody (AB72295)

Detection of CPSF73 by Western Blot of Immunprecipitate. Lane 1 : ab72295, at 0.1μg/ml, staining CPSF73 in HeLa whole cell lysate immunoprecipitated using ab72225 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded).
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-CPSF73 antibody (ab72295)

Predicted band size: 77 kDa

false

Unknown

Western blot - Anti-CPSF73 antibody (AB72295)

All lanes:

Western blot - Anti-CPSF73 antibody (ab72295) at 0.04 µg/mL

Lane 1:

HeLa whole celllysate at 50 µg

Lane 2:

HeLa whole celllysate at 15 µg

Lane 3:

HeLa whole celllysate at 5 µg

Lane 4:

293T whole celllysate at 50 µg

Lane 5:

Mouse NIH3T3 whole celllysate at 50 µg

Predicted band size: 77 kDa

Observed band size: 77 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CPSF3. The exact immunogen used to generate this antibody is proprietary information.

Q9UKF6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72295 was affinity purified using an epitope specific to CPSF73 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPSF73 also known as Cleavage and Polyadenylation Specificity Factor 73 is an important component of the mRNA processing machinery. Mechanically CPSF73 functions as an endonuclease that cleaves pre-mRNA at the 3' end a necessary step for the addition of a poly(A) tail. CPSF73 has a molecular mass of approximately 73 kDa. This protein is expressed ubiquitously across various cell types reflecting its essential role in gene expression regulation.

Biological function summary

The mRNA processing involves CPSF73 as part of the larger cleavage and polyadenylation complex. This complex is responsible for recognizing specific cleavage sites within pre-mRNA and is critical for mRNA maturation. CPSF73’s activity ensures the proper maturation of messenger RNA facilitating its export from the nucleus translation into proteins and subsequent degradation. The endonuclease works closely with other components of the complex including CPSF160 CPSF100 and CFIm suggesting that its role extends beyond just cleavage to broader mRNA stability and regulation.

Pathways

CPSF73 actively participates in the mRNA processing pathway playing an integral role in post-transcriptional regulation. It also interfaces with the spliceosome machinery illustrating the crosstalk between pre-mRNA splicing and polyadenylation sites. CPSF73 is closely related to other RNA processing proteins such as CstF-64 and Symplekin which together maintain the precise regulation of gene expression. This interrelation is particularly important in pathways where timing of mRNA processing impacts cellular function and response.

CPSF73's malfunction could lead to serious implications including cancer and genetic disorders. Aberrant expression or mutations in CPSF73 have been linked to various cancers as they directly affect mRNA maturation and stability which could result in unchecked cell proliferation. Furthermore its connection with proteins such as CPSF160 has been observed in cancers where dysregulation of mRNA processing pathways contribute to tumorigenesis. Disorders like these highlight the necessity of CPSF73 for maintaining cellular health and proper gene expression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as an mRNA 3'-end-processing endonuclease (PubMed : 30507380). Also involved in the histone 3'-end pre-mRNA processing (PubMed : 30507380). U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle (PubMed : 30507380). Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity). Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity). Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity).

See full target information CPSF3

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2507186122 PubMed40768347

2025

RNA polymerase III transcription-associated polyadenylation promotes the accumulation of noncoding retrotransposons during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Azra Lari,Sahil B Shah,Xiaowen Mao,Priyanka Sanghrajka,John Karijolich,Liana F Lareau,Britt A Glaunsinger

Cell death discovery 11:99 PubMed40075082

2025

Deficiency of UBE3D in mice leads to severe embryonic abnormalities and disrupts the mRNA of Homeobox genes via CPSF3.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Mi,Lu Yan,Yu Wu,Yufang Zheng

Nature communications 14:4906 PubMed37582777

2023

mRNA 3'UTR lengthening by alternative polyadenylation attenuates inflammatory responses and correlates with virulence of Influenza A virus.

Applications

Unspecified application

Species

Unspecified reactive species

Valter Bergant,Daniel Schnepf,Niklas de Andrade Krätzig,Philipp Hubel,Christian Urban,Thomas Engleitner,Ronald Dijkman,Bernhard Ryffel,Katja Steiger,Percy A Knolle,Georg Kochs,Roland Rad,Peter Staeheli,Andreas Pichlmair

Nature communications 13:705 PubMed35121750

2022

Population-level deficit of homozygosity unveils CPSF3 as an intellectual disability syndrome gene.

Applications

Unspecified application

Species

Unspecified reactive species

Gudny A Arnadottir,Asmundur Oddsson,Brynjar O Jensson,Svanborg Gisladottir,Mariella T Simon,Asgeir O Arnthorsson,Hildigunnur Katrinardottir,Run Fridriksdottir,Erna V Ivarsdottir,Adalbjorg Jonasdottir,Aslaug Jonasdottir,Rebekah Barrick,Jona Saemundsdottir,Louise le Roux,Gudjon R Oskarsson,Jurate Asmundsson,Thora Steffensen,Kjartan R Gudmundsson,Petur Ludvigsson,Jon J Jonsson,Gisli Masson,Ingileif Jonsdottir,Hilma Holm,Jon G Jonasson,Olafur Th Magnusson,Olafur Thorarensen,Jose Abdenur,Gudmundur L Norddahl,Daniel F Gudbjartsson,Hans T Bjornsson,Unnur Thorsteinsdottir,Patrick Sulem,Kari Stefansson

Molecular and cellular biology 38: PubMed29967245

2018

Heat Shock Causes a Reversible Increase in RNA Polymerase II Occupancy Downstream of mRNA Genes, Consistent with a Global Loss in Transcriptional Termination.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph F Cardiello,James A Goodrich,Jennifer F Kugel

Genes & development 32:127-139 PubMed29432121

2018

Xrn2 accelerates termination by RNA polymerase II, which is underpinned by CPSF73 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua D Eaton,Lee Davidson,David L V Bauer,Toyoaki Natsume,Masato T Kanemaki,Steven West

Nucleic acids research 45:10706-10725 PubMed28977530

2017

Nuclear poly(A) binding protein 1 (PABPN1) and Matrin3 interact in muscle cells and regulate RNA processing.

Applications

Species

Unspecified reactive species

Ayan Banerjee,Katherine E Vest,Grace K Pavlath,Anita H Corbett

View all publications

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Product promise

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Anti-CPSF73 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF73 antibody (AB72295)

Immunoprecipitation - Anti-CPSF73 antibody (AB72295)

Western blot - Anti-CPSF73 antibody (AB72295)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise