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AB131245

Anti-CPSF73 antibody [EPR7468]

5

(2 Reviews)

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(2 Publications)

Rabbit Recombinant Monoclonal CPSF73 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

CPSF73, CPSF3, Cleavage and polyadenylation specificity factor subunit 3, Cleavage and polyadenylation specificity factor 73 kDa subunit, mRNA 3'-end-processing endonuclease CPSF-73, CPSF 73 kDa subunit

5 Images
Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] (AB131245)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] (AB131245)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPSF73 (red) with ab131245 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] (AB131245)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] (AB131245)

Immunofluorescence analysis of HeLa cells labelling CPSF73 with ab131245 at 1/50 dilution.

Western blot - Anti-CPSF73 antibody [EPR7468] (AB131245)
  • WB

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Western blot - Anti-CPSF73 antibody [EPR7468] (AB131245)

All lanes:

Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Lane 6:

PC-12 cell lysate at 10 µg

Lane 7:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 77 kDa

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Western blot - Anti-CPSF73 antibody [EPR7468] (AB131245)
  • WB

AbReview30820****

Western blot - Anti-CPSF73 antibody [EPR7468] (AB131245)

All lanes:

Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution

All lanes:

U2OS whole cell lysate at 100 µg

Predicted band size: 77 kDa

false

OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] (AB131245)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] (AB131245)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-CPSF73 antibody [EPR7468] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7468

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB72295

Anti-CPSF73 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF73 antibody (AB72295)

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Complementary Products

Primary Antibodies

AB72115

Anti-WDR33 antibody

Immunoprecipitation - Anti-WDR33 antibody (AB72115)

  • 0
  • 0
Primary Antibodies

AB248378

Anti-CPSF30/NAR antibody [EPR7433] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-CPSF30/NAR antibody [EPR7433] - BSA and Azide free (AB248378)

  • 0
  • 0
Primary Antibodies

AB248172

Anti-CPSF2/CPSF100 antibody [EPR7599] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-CPSF2/CPSF100 antibody [EPR7599] - BSA and Azide free (AB248172)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/250", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPSF73 also known as Cleavage and Polyadenylation Specificity Factor 73 is an important component of the mRNA processing machinery. Mechanically CPSF73 functions as an endonuclease that cleaves pre-mRNA at the 3' end a necessary step for the addition of a poly(A) tail. CPSF73 has a molecular mass of approximately 73 kDa. This protein is expressed ubiquitously across various cell types reflecting its essential role in gene expression regulation.
Biological function summary

The mRNA processing involves CPSF73 as part of the larger cleavage and polyadenylation complex. This complex is responsible for recognizing specific cleavage sites within pre-mRNA and is critical for mRNA maturation. CPSF73’s activity ensures the proper maturation of messenger RNA facilitating its export from the nucleus translation into proteins and subsequent degradation. The endonuclease works closely with other components of the complex including CPSF160 CPSF100 and CFIm suggesting that its role extends beyond just cleavage to broader mRNA stability and regulation.

Pathways

CPSF73 actively participates in the mRNA processing pathway playing an integral role in post-transcriptional regulation. It also interfaces with the spliceosome machinery illustrating the crosstalk between pre-mRNA splicing and polyadenylation sites. CPSF73 is closely related to other RNA processing proteins such as CstF-64 and Symplekin which together maintain the precise regulation of gene expression. This interrelation is particularly important in pathways where timing of mRNA processing impacts cellular function and response.

CPSF73's malfunction could lead to serious implications including cancer and genetic disorders. Aberrant expression or mutations in CPSF73 have been linked to various cancers as they directly affect mRNA maturation and stability which could result in unchecked cell proliferation. Furthermore its connection with proteins such as CPSF160 has been observed in cancers where dysregulation of mRNA processing pathways contribute to tumorigenesis. Disorders like these highlight the necessity of CPSF73 for maintaining cellular health and proper gene expression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as an mRNA 3'-end-processing endonuclease (PubMed : 30507380). Also involved in the histone 3'-end pre-mRNA processing (PubMed : 30507380). U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle (PubMed : 30507380). Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity). Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity). Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity).
See full target information CPSF3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 20:24 PubMed31997940

2020

SP1 induced long non-coding RNA LINC00958 overexpression facilitate cell proliferation, migration and invasion in lung adenocarcinoma via mediating miR-625-5p/CPSF7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Longhai Yang,Lili Li,Zizi Zhou,Yi Liu,Jinyuan Sun,Xiaoming Zhang,Huiyu Pan,Song Liu

Cell reports 26:2779-2791.e5 PubMed30840897

2019

Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control.

Applications

Unspecified application

Species

Unspecified reactive species

Lee Davidson,Laura Francis,Ross A Cordiner,Joshua D Eaton,Chris Estell,Sara Macias,Javier F Cáceres,Steven West
View all publications
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Product promise

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